BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	130,488	T→C	N258S (AAC→AGC)	yacH ←	DUF3300 domain‑containing protein YacH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	130,488	0	T	C	100.0%	472.3 / NA	130	N258S (AAC→AGC)	yacH	DUF3300 domain‑containing protein YacH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (55/75); total (55/75)

Only 100 of 131 total aligned reads displayed.

CCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGG  >  NC_000913/130351‑130624

cccAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTc                                                                                                                                        >  1:612842/1‑140 (MQ=255)

cccAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTc                                                                                                                                        >  1:1377989/1‑140 (MQ=255)

     tCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTa                                                                                                                                   <  1:1047083/140‑1 (MQ=255)

        aTGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAAc                                                                                                                                <  1:1168961/140‑1 (MQ=255)

         tgctgcTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAAcc                                                                                                                               >  2:326702/1‑140 (MQ=255)

          gctgctGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCa                                                                                                                              <  2:1271168/140‑1 (MQ=255)

              ctgAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggt                                                                                                                          >  1:815978/1‑140 (MQ=255)

              ctgAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggt                                                                                                                          <  2:1377989/140‑1 (MQ=255)

              ctgAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggt                                                                                                                          <  2:280495/140‑1 (MQ=255)

                   tAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAAc                                                                                                                                <  1:279016/129‑1 (MQ=255)

                   tAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAAc                                                                                                                                >  2:279016/1‑129 (MQ=255)

                   tAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggttgggt                                                                                                                     >  1:189752/1‑140 (MQ=255)

                     gTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggttgggttg                                                                                                                   <  2:1622446/140‑1 (MQ=255)

                     gTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggttgggttg                                                                                                                   <  2:448156/140‑1 (MQ=255)

                      tGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAAc                                                                                                                                <  2:116365/126‑1 (MQ=255)

                      tGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAAc                                                                                                                                >  1:116365/1‑126 (MQ=255)

                      tGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCa                                                                                                                              >  2:1453838/1‑128 (MQ=255)

                      tGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGt                                                                                                                  >  2:481803/1‑140 (MQ=255)

                      tGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGt                                                                                                                  >  2:100528/1‑140 (MQ=255)

                      tGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGt                                                                                                                  >  1:582321/1‑140 (MQ=255)

                       gCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTa                                                                                                                 <  1:100528/140‑1 (MQ=255)

                        cGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACggtt                                                                                                                         >  1:100545/1‑131 (MQ=255)

                        cGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAg                                                                                                                >  1:1293746/1‑140 (MQ=255)

                        cGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAg                                                                                                                >  1:539246/1‑140 (MQ=255)

                           aCGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTg                                                                                                             >  1:1339159/1‑140 (MQ=255)

                            cGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTgg                                                                                                            <  2:1339159/140‑1 (MQ=255)

                            cGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTgg                                                                                                            <  2:1160388/140‑1 (MQ=255)

                            cGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTgg                                                                                                            <  2:969643/140‑1 (MQ=255)

                                 tAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAata                                                                                                       <  1:864293/140‑1 (MQ=255)

                                 tAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAata                                                                                                       <  2:915019/140‑1 (MQ=255)

                                  aGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAatat                                                                                                      >  2:1464186/1‑140 (MQ=255)

                                    cAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAg                                                                                                    <  2:1600839/140‑1 (MQ=255)

                                     aaCGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGa                                                                                                   <  2:614708/140‑1 (MQ=255)

                                     aaCGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGa                                                                                                   <  1:1521906/140‑1 (MQ=255)

                                      aCGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGAc                                                                                                  <  2:314832/140‑1 (MQ=255)

                                      aCGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGAc                                                                                                  <  1:503217/140‑1 (MQ=255)

                                       cGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGAcc                                                                                                 >  2:544310/1‑140 (MQ=255)

                                       cGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGAcc                                                                                                 >  2:1119569/1‑140 (MQ=255)

                                          ccATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACcaca                                                                                              <  1:1119569/140‑1 (MQ=255)

                                          ccATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACcaca                                                                                              <  1:544310/140‑1 (MQ=255)

                                           cATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACAt                                                                                             <  2:539246/140‑1 (MQ=255)

                                             tGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCa                                                                                           >  1:1357252/1‑140 (MQ=255)

                                              gCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAg                                                                                          <  1:326702/140‑1 (MQ=255)

                                                  taGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGAtt                                                                                      >  1:1149133/1‑140 (MQ=255)

                                                  taGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGAtt                                                                                      >  1:971671/1‑140 (MQ=255)

                                                    gCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGAAACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAGTATAGACCACATCAGGATTgg                                                                                    >  2:1477884/1‑140 (MQ=255)

                                                     ccGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGc                                                                                   <  1:987083/140‑1 (MQ=255)

                                                       gAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCgg                                                                                 <  2:815978/140‑1 (MQ=255)

                                                         aTCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGc                                                                               >  1:944909/1‑140 (MQ=255)

                                                          tCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCt                                                                              >  2:1409606/1‑140 (MQ=255)

                                                          tCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCt                                                                              >  2:1586724/1‑140 (MQ=255)

                                                          tCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCt                                                                              <  1:1409606/140‑1 (MQ=255)

                                                          tCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCt                                                                              >  2:890877/1‑140 (MQ=255)

                                                            cgcgTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCa                                                                            >  1:879214/1‑140 (MQ=255)

                                                              cgTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAAt                                                                          >  2:292647/1‑140 (MQ=255)

                                                              cgTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAAt                                                                          >  1:595223/1‑140 (MQ=255)

                                                               gTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATg                                                                         <  1:1372158/140‑1 (MQ=255)

                                                                    aaGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAAt                                                                    <  1:34525/140‑1 (MQ=255)

                                                                    aaGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAAt                                                                    >  1:84981/1‑140 (MQ=255)

                                                                     aGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATg                                                                   >  1:1286629/1‑140 (MQ=255)

                                                                      gCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGa                                                                  <  1:1477884/140‑1 (MQ=255)

                                                                      gCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGa                                                                  <  2:163793/140‑1 (MQ=255)

                                                                      gCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGa                                                                  <  2:659869/140‑1 (MQ=255)

                                                                         gTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACgg                                                               >  1:1610494/1‑140 (MQ=255)

                                                                             acaaACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATg                                                                         >  2:699672/1‑126 (MQ=255)

                                                                             acaaACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATAGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGgttgt                                                           >  2:375985/1‑140 (MQ=255)

                                                                              caaACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGgttgtt                                                          <  2:595223/140‑1 (MQ=255)

                                                                               aaaCGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGttgttg                                                         >  1:1076316/1‑140 (MQ=255)

                                                                               aaaCGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGttgttg                                                         >  2:665825/1‑140 (MQ=255)

                                                                                aaCGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGc                                                        <  1:890877/140‑1 (MQ=255)

                                                                                aaCGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGc                                                        <  2:1149133/140‑1 (MQ=255)

                                                                                aaCGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGc                                                        <  1:12526/140‑1 (MQ=255)

                                                                                aaCGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGc                                                        <  2:1205086/140‑1 (MQ=255)

                                                                                   ggTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCgg                                                     <  1:1625594/140‑1 (MQ=255)

                                                                                   ggTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCgg                                                     <  2:1307948/140‑1 (MQ=255)

                                                                                    gTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGc                                                    <  1:370683/140‑1 (MQ=255)

                                                                                     ttCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGct                                                   <  1:1464186/140‑1 (MQ=255)

                                                                                                gtggtggCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACg                                        <  2:1610494/140‑1 (MQ=255)

                                                                                                      gCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTg                                  <  2:84981/140‑1 (MQ=255)

                                                                                                      gCAGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTg                                  <  2:1170269/140‑1 (MQ=255)

                                                                                                        aGATAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGc                                <  2:501819/140‑1 (MQ=255)

                                                                                                          aTAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTg                                                <  1:1115878/122‑1 (MQ=255)

                                                                                                          aTAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTg                                                >  2:1115878/1‑122 (MQ=255)

                                                                                                          aTAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAg                              >  1:219419/1‑140 (MQ=255)

                                                                                                          aTAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAg                              >  2:1263439/1‑140 (MQ=255)

                                                                                                          aTAAACCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGCTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTg                                  >  1:311863/1‑136 (MQ=255)

                                                                                                             aaCCGGCGGATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTa                           >  2:319795/1‑140 (MQ=255)

                                                                                                                   cggATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTg                     <  1:51640/140‑1 (MQ=255)

                                                                                                                     gATACGCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGATGGGGTTGGCAGTTAAAACTGTa                   <  1:40647/140‑1 (MQ=255)

                                                                                                                          gtaGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGa              <  2:1503661/138‑1 (MQ=255)

                                                                                                                          gCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGa              <  2:219419/140‑1 (MQ=255)

                                                                                                                          gCAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGa              <  2:189752/140‑1 (MQ=255)

                                                                                                                           cAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGAt             <  2:1357252/140‑1 (MQ=255)

                                                                                                                           cAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGAt             <  1:1605709/140‑1 (MQ=255)

                                                                                                                           cAGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGAt             <  1:265340/140‑1 (MQ=255)

                                                                                                                            aGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGa              >  1:1256356/1‑138 (MQ=255)

                                                                                                                            aGTATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGa              <  2:1256356/138‑1 (MQ=255)

                                                                                                                              tATTGGCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGt          >  1:1310749/1‑140 (MQ=255)

                                                                                                                                   gCCCAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGAc     >  1:74141/1‑140 (MQ=255)

                                                                                                                                      cAGCTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACggg  <  1:1206767/140‑1 (MQ=255)

CCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTGGTGGTGGCAGATAAACCGGCGGATACGCAGTATTGGCCCAGTTCCCGTAAACCACGGTTGGGTTGTAGTTGGGAATATAGACCACATCAGGATTGGCGGGCTCAATGGAAATGACGGTTGTTGCCGGCTCTGTAATGACGGGGTTGGCAGTTAAAACTGTATTGGATGGTATGACGGG  >  NC_000913/130351‑130624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`