Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,254,839	A→G	W372R (TGG→CGG)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,254,839	0	A	G	100.0%	276.6 / NA	77	W372R (TGG→CGG)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/35);  total (42/35)

GTCGGGTGTACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGC  >  NC_000913/1254702‑1254973                                                                                                                                           |                                                                                                                                       gTCGGGTGTACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCTCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCcgca                                                                                                                                      >  1:1371693/1‑140 (MQ=255) gTCGGGTGTACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCcgca                                                                                                                                      >  2:1444244/1‑140 (MQ=255)     ggTGTACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAAcc                                                                                                                                  <  1:417136/140‑1 (MQ=255)         tACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAgc                                                                                                                              >  1:1068990/1‑140 (MQ=255)         tACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAgc                                                                                                                              >  1:1472211/1‑140 (MQ=255)          aCTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAgcg                                                                                                                             <  2:559597/140‑1 (MQ=255)           cTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAgcgc                                                                                                                            >  1:1359057/1‑140 (MQ=255)            tCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGcc                                                                                                                           >  1:863689/1‑140 (MQ=255)                atCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCTCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGAc                                                                                                                       <  2:1371693/140‑1 (MQ=255)                atCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGAc                                                                                                                       >  2:1275554/1‑140 (MQ=255)                atCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGAc                                                                                                                       >  2:1452138/1‑140 (MQ=255)                   aaTGCCCAGTTCGCTGGTGAGGCCGATCGGGCTGTGGGCCATGCCATAATTTCCCCCGGCACCCGCCGCGCCGGTGAAAGCAAACCTGT‑CTCTGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGc                                                                                                                    <  2:227212/140‑1 (MQ=255)                     tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCa                                                                                                                          >  1:156643/1‑132 (MQ=255)                     tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCa                                                                                                                          <  2:156643/132‑1 (MQ=255)                     tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCa                                                                                                                  <  1:1275554/140‑1 (MQ=255)                     tGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCa                                                                                                                  <  1:697059/140‑1 (MQ=255)                      gACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCAt                                                                                                                 >  1:266868/1‑140 (MQ=255)                        ccAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTa                                                                                                               <  1:1503644/140‑1 (MQ=255)                          aGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACg                                                                                                             <  1:385449/140‑1 (MQ=255)                          aGATCGCTCGGGAGGTCGATCTGGCTGTTGGTCATGGCATAATTCCCCCTGCCCCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACg                                                                                                             <  2:1603862/140‑1 (MQ=255)                           gATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGa                                                                                                            <  1:1452138/140‑1 (MQ=255)                            aTCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGAt                                                                                                           >  2:268208/1‑140 (MQ=255)                            aTCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCCCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGAt                                                                                                           <  1:152144/140‑1 (MQ=255)                             tCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATc                                                                                                          <  2:1471792/140‑1 (MQ=255)                             tCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATc                                                                                                          <  2:1359057/140‑1 (MQ=255)                             tCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATc                                                                                                          <  2:887666/140‑1 (MQ=255)                               gCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCaa                                                                                                        >  1:1090930/1‑140 (MQ=255)                                cTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAAc                                                                                                       >  2:707321/1‑140 (MQ=255)                                cTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAAc                                                                                                       >  1:1149522/1‑140 (MQ=255)                                cTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAAc                                                                                                       >  1:1063924/1‑140 (MQ=255)                                 tCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACt                                                                                                      >  2:846593/1‑140 (MQ=255)                                  cGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTg                                                                                                     >  2:1012962/1‑140 (MQ=255)                                      aGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTa                                                                                                               <  2:731754/126‑1 (MQ=255)                                      aGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTa                                                                                                               >  1:731754/1‑126 (MQ=255)                                      aGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAat                                                                                                 >  2:1280850/1‑140 (MQ=255)                                           gATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAata                                                                                                <  2:293029/136‑1 (MQ=255)                                           gATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAata                                                                                                >  1:293029/1‑136 (MQ=255)                                             tCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTa                                                                                          <  2:1086431/140‑1 (MQ=255)                                                   tGTTGGTCATGGCATAAATCCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGc                                                                                    <  1:268208/140‑1 (MQ=255)                                                     ttGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCAt                                                                                  >  2:141701/1‑140 (MQ=255)                                                       ggTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGGCTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGt                                                                                >  2:853821/1‑140 (MQ=255)                                                       ggTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGt                                                                                >  2:251687/1‑140 (MQ=255)                                                       ggTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGt                                                                                >  2:252575/1‑140 (MQ=255)                                                           aTGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGac                                                                            >  1:356382/1‑140 (MQ=255)                                                               cATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGacaaca                                                                        >  2:1425848/1‑140 (MQ=255)                                                                        cccTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGcc                                                               >  1:344652/1‑140 (MQ=255)                                                                         ccTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCg                                                              >  2:153019/1‑140 (MQ=255)                                                                           tGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGGCTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTc                                                            <  1:853821/140‑1 (MQ=255)                                                                           gggCCCCTGCCGCGCCGGTGATAGCCAACCTGT‑CTCCGGTTTTTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTc                                                            <  2:1253009/139‑1 (MQ=255)                                                                             gCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTg                                                          <  1:1444244/140‑1 (MQ=255)                                                                             gCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTg                                                          >  2:1330302/1‑140 (MQ=255)                                                                             gCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTg                                                          >  2:277372/1‑140 (MQ=255)                                                                                  tGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCg                                                     >  1:531009/1‑140 (MQ=255)                                                                                   gCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGa                                                    <  2:1063924/140‑1 (MQ=255)                                                                                    ccGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAg                                                   <  1:153019/140‑1 (MQ=255)                                                                                     cgcgCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAgg                                                  <  1:1339020/140‑1 (MQ=255)                                                                                     cgcgCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAgg                                                  <  1:707321/140‑1 (MQ=255)                                                                                     cgcgCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAgg                                                  <  1:846593/140‑1 (MQ=255)                                                                                      gcgcGGGTGATAGCCAACCTGT‑CCCCGTTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTCGACAACAGCCGTTGCCGTCTGGGCCGAGGc                                                 <  2:1579791/140‑1 (MQ=255)                                                                                      gcgcCGGTGATACCCAACCTGC‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGc                                                 <  2:36551/140‑1 (MQ=255)                                                                                       cgcCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCa                                                >  1:36551/1‑140 (MQ=255)                                                                                       cgcCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTCGACAACAGCCGTTGCCGTCTGGGCCGAGGCa                                                >  1:1579791/1‑140 (MQ=255)                                                                                         ccGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCAcc                                              >  1:1210993/1‑140 (MQ=255)                                                                                           ggTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCAt                                            >  2:1465980/1‑140 (MQ=255)                                                                                                atcccAAACTGTCCTCCGGTTTTTCTCCCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGa                                        <  2:1534076/137‑1 (MQ=255)                                                                                                   ccAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccg                                    >  2:1372824/1‑140 (MQ=255)                                                                                                   ccAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccg                                    >  2:683107/1‑140 (MQ=255)                                                                                                     aaaCTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgcc                                  <  1:251687/140‑1 (MQ=255)                                                                                                      aaCTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgccg                                 >  2:173825/1‑140 (MQ=255)                                                                                                       aCTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCgg                                >  2:800302/1‑140 (MQ=255)                                                                                                        cTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGa                               <  1:1372824/140‑1 (MQ=255)                                                                                                                  gTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCt                      >  2:58414/1‑140 (MQ=255)                                                                                                                  gTTATTCTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCt                      >  2:83523/1‑140 (MQ=255)                                                                                                                        cTACCGCCGCTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCg                <  1:277372/140‑1 (MQ=255)                                                                                                                            cgccgcTGAGAGCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTgc            >  1:459397/1‑140 (MQ=255)                                                                                                                                    agagCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGGGGCACCATAAGAACCACCGGAAAAGATGCTGTTTCGTTGCGCTGCCGt    >  1:1058653/1‑140 (MQ=255)                                                                                                                                     gagCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTg   >  2:1162748/1‑140 (MQ=255)                                                                                                                                      agCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGc  <  1:1280850/140‑1 (MQ=255)                                                                                                                                      agCCCGCAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGc  <  1:1012962/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       GTCGGGTGTACTCATATCAATGACCAGATCGCTCGTGAGGTCGATCTGGCTGTTGGTCATGGCATAAATTCCCCTGGCTCCTGCCGCGCCGGTGATAGCCAAACTGT‑CTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGC  >  NC_000913/1254702‑1254973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑