Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,437	C→T	intergenic (‑96/+53)	ymjE ← / ← puuA	protein YmjE/glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,437	0	C	T	100.0%	213.8 / NA	61	intergenic (‑96/+53)	ymjE/puuA	protein YmjE/glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/32);  total (29/32)

GCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTG  >  NC_000913/1359303‑1359573                                                                                                                                       |                                                                                                                                         gCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATg                                                                                                                                     <  2:293164/140‑1 (MQ=255)        ccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGcc                                                                                                                              <  2:996352/140‑1 (MQ=255)        ccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGcc                                                                                                                              <  2:649906/140‑1 (MQ=255)        ccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGcc                                                                                                                              <  2:1591645/140‑1 (MQ=255)        ccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGcc                                                                                                                              <  2:1558322/140‑1 (MQ=255)        ccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGcc                                                                                                                              <  2:1519449/140‑1 (MQ=255)         cctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCt                                                                                                                             >  1:109424/1‑140 (MQ=255)         cctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCt                                                                                                                             >  2:1145562/1‑140 (MQ=255)         cctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCt                                                                                                                             >  1:549754/1‑140 (MQ=255)         cctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCt                                                                                                                             >  1:306224/1‑140 (MQ=255)           tcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTgg                                                                                                                           >  1:1574525/1‑140 (MQ=255)             gCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCt                                                                                                                         <  1:724158/140‑1 (MQ=255)                aTGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCg                                                                                                                      >  1:1386267/1‑140 (MQ=255)                aTGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCg                                                                                                                      >  1:382960/1‑140 (MQ=255)                  gCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCt                                                                                                                    >  2:1034138/1‑140 (MQ=255)                  gCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCt                                                                                                                    <  2:1574525/140‑1 (MQ=255)                   cGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTc                                                                                                                   >  2:1581426/1‑140 (MQ=255)                   cGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTc                                                                                                                   >  2:201095/1‑140 (MQ=255)                       gATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGc                                                                                                               >  2:1332158/1‑140 (MQ=255)                         tttAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCg                                                                                                             >  2:622284/1‑140 (MQ=255)                                   ggCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAaacaac                                                                                                   >  2:77540/1‑140 (MQ=255)                                     catcatCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAAccc                                                                                                 <  2:382960/140‑1 (MQ=255)                                     catcatCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAAccc                                                                                                 <  2:1386267/140‑1 (MQ=255)                                        catcTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGc                                                                                              <  1:201095/140‑1 (MQ=255)                                          tctcATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCaa                                                                                            <  2:1327936/140‑1 (MQ=255)                                            tcATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAgg                                                                                          <  1:1581426/140‑1 (MQ=255)                                                  ccTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTAtt                                                                                    >  1:392146/1‑140 (MQ=255)                                                     cGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcg                                                                                 <  2:495051/140‑1 (MQ=255)                                                     cGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcg                                                                                 >  2:1128530/1‑140 (MQ=255)                                                     cGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcg                                                                                 <  1:622284/140‑1 (MQ=255)                                                     cGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcg                                                                                 >  2:1114067/1‑140 (MQ=255)                                                             tCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTc                                                                         <  1:1034138/140‑1 (MQ=255)                                                                   tCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATc                                                                   <  1:166642/140‑1 (MQ=255)                                                                     gTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCa                                                                 >  1:432669/1‑140 (MQ=255)                                                                       tcaCGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCGGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACt                                                               <  2:1504491/138‑1 (MQ=255)                                                                       tAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACt                                                               >  1:1134839/1‑140 (MQ=255)                                                                       tAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACt                                                               >  1:496431/1‑140 (MQ=255)                                                                       tAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACt                                                               >  1:1504491/1‑140 (MQ=255)                                                                       tAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTAGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACt                                                               >  2:480591/1‑140 (MQ=255)                                                                        aaCGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTc                                                              >  1:263568/1‑140 (MQ=255)                                                                               gCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATc                                                                   >  1:1542468/1‑128 (MQ=255)                                                                               gCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATc                                                                   <  2:1542468/128‑1 (MQ=255)                                                                                cccGTTGCCGCCCGCGCTCCATAACGCGACATCCGTGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAAtttctg                                                      >  1:474114/1‑140 (MQ=255)                                                                                   gTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAAtttctgttt                                                   <  1:336881/140‑1 (MQ=255)                                                                                   gTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAAtttctgttt                                                   <  2:306224/140‑1 (MQ=255)                                                                                        cgcccgcGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCtgtg                                              <  2:663745/140‑1 (MQ=255)                                                                                          cccgcGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGtgat                                            <  1:57893/140‑1 (MQ=255)                                                                                           ccgcGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCTGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGtgatg                                           >  1:1484874/1‑140 (MQ=255)                                                                                              cgcTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAgg                                        >  1:1368008/1‑140 (MQ=255)                                                                                                           gACATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCa                           >  2:667119/1‑140 (MQ=255)                                                                                                             cATCCGGGAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAAc                         >  2:197326/1‑140 (MQ=255)                                                                                                                  gggAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCtc                    <  1:1145562/140‑1 (MQ=255)                                                                                                                   ggAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCtca                   <  1:1128530/140‑1 (MQ=255)                                                                                                                    gAAATGTCTGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCtcat                  <  1:10307/140‑1 (MQ=255)                                                                                                                            tGGATTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTACAACAGCTCATCATTTTTa          <  1:1190953/140‑1 (MQ=255)                                                                                                                               aTTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAg       <  2:549754/140‑1 (MQ=255)                                                                                                                               aTTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAg       <  1:1114067/140‑1 (MQ=255)                                                                                                                               aTTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAg       <  1:480591/140‑1 (MQ=255)                                                                                                                               aTTACCAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAg       <  2:1368008/140‑1 (MQ=255)                                                                                                                                   ccAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGt   >  1:582556/1‑140 (MQ=255)                                                                                                                                    cAGTAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTg  <  2:432669/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTG  >  NC_000913/1359303‑1359573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑