Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,980	A→G	G711G (GGT→GGC)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,980	0	A	G	100.0%	288.7 / NA	80	G711G (GGT→GGC)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/34);  total (46/34)

CGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGACCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCAC  >  NC_000913/1784844‑1785110                                                                                                                                         |                                                                                                                                   cggacggaCCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCg                                                                                                                                 >  1:893/1‑140 (MQ=255) cggacggaCCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCg                                                                                                                                 >  1:536443/1‑140 (MQ=255) cggacggaCCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCg                                                                                                                                 >  2:1422899/1‑140 (MQ=255)   gacggacCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAg                                                                                                                               >  2:1597409/1‑140 (MQ=255)   gacggacCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAg                                                                                                                               >  2:1250958/1‑140 (MQ=255)   gacggacCCTGACCGCAAATCCCGACATATTTGCCCCGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAg                                                                                                                               >  2:1327396/1‑140 (MQ=255)         cccTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAg                                                                                                                         <  2:700124/140‑1 (MQ=255)         cccTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAg                                                                                                                         <  2:894084/140‑1 (MQ=255)             gACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGc                                                                                                                     >  2:614546/1‑140 (MQ=255)               ccGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGt                                                                                                                   <  2:1196888/140‑1 (MQ=255)                  cAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCat                                                                                                                >  1:1340369/1‑140 (MQ=255)                        ccGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGtt                                                                                                          <  2:1340369/140‑1 (MQ=255)                              tatTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGcc                                                                                                    <  1:878692/140‑1 (MQ=255)                                     ccTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACGCCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTgag                                                                                             <  1:881622/140‑1 (MQ=255)                                       tGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTgaga                                                                                            <  1:857490/139‑1 (MQ=255)                                       tGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTgaga                                                                                            >  2:857490/1‑139 (MQ=255)                                        gTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAg                                                                                          >  2:317877/1‑140 (MQ=255)                                        gTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAg                                                                                          >  1:428240/1‑140 (MQ=255)                                         tttCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGc                                                                                         >  2:1348923/1‑140 (MQ=255)                                         tttCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGc                                                                                         >  1:21240/1‑140 (MQ=255)                                         tttCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGc                                                                                         >  2:1379569/1‑140 (MQ=255)                                          ttcttcGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGcc                                                                                        >  1:1419878/1‑140 (MQ=255)                                          ttcttcGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGcc                                                                                        >  2:1414395/1‑140 (MQ=255)                                              tcGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCg                                                                                    <  1:1327396/140‑1 (MQ=255)                                               cgcgGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGa                                                                                   >  1:748363/1‑140 (MQ=255)                                                gcgGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGaa                                                                                  <  2:576854/140‑1 (MQ=255)                                                  ggCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAat                                                                                <  2:893/140‑1 (MQ=255)                                                  ggCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAat                                                                                <  2:748363/140‑1 (MQ=255)                                                  ggCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAat                                                                                <  2:536443/140‑1 (MQ=255)                                                   gCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAata                                                                               >  2:557636/1‑140 (MQ=255)                                                   gCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAata                                                                               >  2:1084403/1‑140 (MQ=255)                                                   gCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAata                                                                               >  1:662301/1‑140 (MQ=255)                                                   gCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAata                                                                               >  2:1233792/1‑140 (MQ=255)                                                       ggATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCg                                                                           <  2:980479/140‑1 (MQ=255)                                                           aGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGa                                                                       <  1:977023/140‑1 (MQ=255)                                                              cATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACt                                                                    >  1:395632/1‑140 (MQ=255)                                                                tCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGc                                                                  <  2:976358/140‑1 (MQ=255)                                                                tCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGc                                                                  <  1:1422899/140‑1 (MQ=255)                                                                 cGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCt                                                                 <  2:395632/140‑1 (MQ=255)                                                                 cGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCt                                                                 <  1:557636/140‑1 (MQ=255)                                                                  gACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTc                                                                <  2:1419878/140‑1 (MQ=255)                                                                   aCAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCg                                                               >  2:108002/1‑140 (MQ=255)                                                                    cagcagTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGTGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAAcatttcga                                                              <  1:392155/140‑8 (MQ=255)                                                                     agcagTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATAtt                                                                             <  1:1257477/124‑1 (MQ=255)                                                                     agcagTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATAtt                                                                             >  2:1257477/1‑124 (MQ=255)                                                                         gTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGc                                                         >  2:1515822/1‑140 (MQ=255)                                                                         gTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGc                                                         <  1:614546/140‑1 (MQ=255)                                                                          tccTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCa                                                        <  2:1100087/138‑1 (MQ=255)                                                                          tGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCa                                                        >  2:1163860/1‑140 (MQ=255)                                                                          tGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCa                                                        >  1:1040005/1‑140 (MQ=255)                                                                          tGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCa                                                        <  1:1170422/140‑1 (MQ=255)                                                                            cTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAg                                                      >  1:1481901/1‑140 (MQ=255)                                                                            cTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAg                                                      >  1:1139535/1‑140 (MQ=255)                                                                                 aCCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGtt                                                 >  2:909031/1‑140 (MQ=255)                                                                                 aCCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGtt                                                 >  1:1402235/1‑140 (MQ=255)                                                                                    gCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGa                                              >  2:1610788/1‑140 (MQ=255)                                                                                    gCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGa                                              <  1:1379569/140‑1 (MQ=255)                                                                                     cATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGAc                                             >  1:63452/1‑140 (MQ=255)                                                                                      aTCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACg                                            >  2:1501256/1‑140 (MQ=255)                                                                                      aTCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACg                                            <  1:108002/140‑1 (MQ=255)                                                                                      aTCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACg                                            >  1:1441746/1‑140 (MQ=255)                                                                                      aTCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACg                                            <  2:37621/140‑1 (MQ=255)                                                                                        cGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACggg                                          <  1:1414395/140‑1 (MQ=255)                                                                                          ttGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGAt                                        >  1:1010680/1‑140 (MQ=255)                                                                                          ttGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCACATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGAt                                        >  2:1011200/1‑140 (MQ=255)                                                                                              gcTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTca                                    <  1:1597409/140‑1 (MQ=255)                                                                                                       aCAATTCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCatg                           <  1:1084403/140‑1 (MQ=255)                                                                                                           ttCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCAtgatga                       >  1:947019/1‑140 (MQ=255)                                                                                                            tCAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATgatgat                      >  1:352865/1‑140 (MQ=255)                                                                                                             cAGACACCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCGGCAAGGCGTTGGACGGGATTTCACACATCATGATGAtt                     <  1:528072/140‑1 (MQ=255)                                                                                                                acacCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTc                  >  2:851656/1‑140 (MQ=255)                                                                                                                acacCACGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTc                  >  1:129744/1‑140 (MQ=255)                                                                                                                   ccacGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGc               >  2:248488/1‑140 (MQ=255)                                                                                                                     acGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGccc             <  1:1515822/140‑1 (MQ=255)                                                                                                                     acGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGccc             >  1:1507015/1‑140 (MQ=255)                                                                                                                      cGCCGGAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCg            >  2:988746/1‑140 (MQ=255)                                                                                                                          ggAGTCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTtct        <  2:428240/140‑1 (MQ=255)                                                                                                                              tCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGcc    >  2:1521448/1‑140 (MQ=255)                                                                                                                              tCACGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGcc    >  1:467117/1‑140 (MQ=255)                                                                                                                                aCGGTCCAGGCCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCAc  <  1:1000734/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGCAGTGCTTTCACCGCATCGTTGCGCTCATCGAACAATTCAGACACCACGCCGGAGTCACGGTCCAGACCGAGCGCCAGCTGCGTCATATCGTTTGAGCCAATTGAGAAGCCGTCGAAATATTCGAGGAACTGCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCAC  >  NC_000913/1784844‑1785110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑