Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,846,485	C→T	C159Y (TGC→TAC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,846,485	0	C	T	100.0%	275.7 / NA	81	C159Y (TGC→TAC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/40);  total (41/40)

CCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGCACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTC  >  NC_000913/1846349‑1846621                                                                                                                                         |                                                                                                                                         cccAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGtaca                                                                                                                                       <  2:528380/140‑1 (MQ=255)   cAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGc                                                                                                                                     <  1:468830/140‑1 (MQ=255)   cAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGc                                                                                                                                     <  2:235068/140‑1 (MQ=255)           ggCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGtaca                                                                                                                                       >  1:103561/1‑130 (MQ=255)           ggCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGtaca                                                                                                                                       <  2:103561/130‑1 (MQ=255)           ggCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGtaca                                                                                                                                       <  2:1608234/130‑1 (MQ=255)           ggCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGtaca                                                                                                                                       >  1:1608234/1‑130 (MQ=255)           ggCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACaaa                                                                                                                             >  1:172517/1‑140 (MQ=255)           ggCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACaaa                                                                                                                             >  2:1127881/1‑140 (MQ=255)             cGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACt                                                                                                                           <  2:832726/140‑1 (MQ=255)                           ccACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGa                                                                                                             >  2:269414/1‑140 (MQ=255)                               ggAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGAc                                                                                                         <  1:1233176/140‑1 (MQ=255)                               ggAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGAc                                                                                                         <  2:1432868/140‑1 (MQ=255)                               ggAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGAc                                                                                                         <  1:1299661/140‑1 (MQ=255)                                     tACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGa                                                                                                   >  1:1367294/1‑140 (MQ=255)                                       cGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATc                                                                                                 <  1:42014/140‑1 (MQ=255)                                       cGCCCTGATAACCGGCATTGCGACCGAGAATGGCATACGCACGGGTCATATTGATGCCGTACAGCCAGCCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATc                                                                                                 >  2:534812/1‑140 (MQ=255)                                         cccTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGc                                                                                               >  2:47771/1‑140 (MQ=255)                                          ccTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGcc                                                                                              <  1:1163978/140‑1 (MQ=255)                                           cTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGccc                                                                                             >  1:225938/1‑140 (MQ=255)                                             gATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGc                                                                                           >  1:1144677/1‑140 (MQ=255)                                                 aCCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCaa                                                                                       <  2:202459/140‑1 (MQ=255)                                                   cGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAAcc                                                                                     <  1:978908/140‑1 (MQ=255)                                                   cGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAAcc                                                                                     <  1:1404190/140‑1 (MQ=255)                                                      cATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGcc                                                                                  >  1:1084679/1‑140 (MQ=255)                                                      cATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGcc                                                                                  >  2:478526/1‑140 (MQ=255)                                                       aTTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCt                                                                                 <  1:478526/140‑1 (MQ=255)                                                       aTTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCt                                                                                 <  2:267562/140‑1 (MQ=255)                                                        ttGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTg                                                                                <  2:225938/140‑1 (MQ=255)                                                        ttGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTg                                                                                >  1:1026082/1‑140 (MQ=255)                                                        ttGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTg                                                                                >  1:396785/1‑140 (MQ=255)                                                        ttGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTg                                                                                <  2:1340380/140‑1 (MQ=255)                                                        ttGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTg                                                                                <  1:1294311/140‑1 (MQ=255)                                                              ccGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACCTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGtt                                                                          <  2:1429113/140‑1 (MQ=255)                                                                    aTGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTc                                                                    <  2:1144677/140‑1 (MQ=255)                                                                     gggTATCCGCCCGGGGTATATTAATGCGGTACGGCCAGTCGGCACGCGCTCGCGCCGGCGCAGAAACGTACACCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAGCCGCCTGCGGGTTCAGGTCg                                                                   <  2:1084679/139‑1 (MQ=255)                                                                          taCGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTAt                                                              <  1:269414/140‑1 (MQ=255)                                                                            cGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCa                                                            >  2:365493/1‑140 (MQ=255)                                                                            cGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCa                                                            >  1:839742/1‑140 (MQ=255)                                                                             gCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCaa                                                           >  2:704929/1‑140 (MQ=255)                                                                               aCGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTc                                                                         <  1:127448/124‑1 (MQ=255)                                                                               aCGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTc                                                                         >  2:127448/1‑124 (MQ=255)                                                                               aCGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGc                                                         <  2:172517/140‑1 (MQ=255)                                                                               aCGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGc                                                         <  2:927923/140‑1 (MQ=255)                                                                               aCGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGc                                                         <  2:1309316/140‑1 (MQ=255)                                                                                cGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCa                                                        >  1:67125/1‑140 (MQ=255)                                                                                cGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCa                                                        >  2:1226567/1‑140 (MQ=255)                                                                                 gggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCaa                                                       >  2:756568/1‑140 (MQ=255)                                                                                 gggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCaa                                                       >  2:1109640/1‑140 (MQ=255)                                                                                 gggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCaa                                                       >  2:634056/1‑140 (MQ=255)                                                                                 gggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCaa                                                       >  2:583790/1‑140 (MQ=255)                                                                                 gggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCaa                                                       <  1:1127881/140‑1 (MQ=255)                                                                                 gggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCaa                                                       >  1:1179876/1‑140 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGc                                                         >  2:1228833/1‑137 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGc                                                         <  1:1228833/137‑1 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAAc                                                      >  2:1075554/1‑140 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAAc                                                      >  1:1068523/1‑140 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAAc                                                      >  2:618344/1‑140 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAAc                                                      >  1:1500592/1‑140 (MQ=255)                                                                                  ggTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCATTTATCAAGCAAc                                                      >  1:139623/1‑140 (MQ=255)                                                                                       tatTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGt                                                 >  2:1216144/1‑140 (MQ=255)                                                                                        atTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTa                                                >  2:1064084/1‑140 (MQ=255)                                                                                             tGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACctgc                                           >  1:169552/1‑140 (MQ=255)                                                                                             tGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACctgc                                           >  1:1113977/1‑140 (MQ=255)                                                                                             tGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACctgc                                           >  1:1505769/1‑140 (MQ=255)                                                                                               ccGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACctgctg                                         >  2:1213773/1‑140 (MQ=255)                                                                                                 gTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCCGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGgc                                       >  2:1625886/1‑140 (MQ=255)                                                                                                  tACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGgcg                                      >  1:333134/1‑140 (MQ=255)                                                                                                    cAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCt                                    <  2:691167/140‑1 (MQ=255)                                                                                                    cAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTACCAAGCAACGCTGTACCTGCTGGCGCt                                    >  1:315280/1‑140 (MQ=255)                                                                                                        cAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTc                                <  1:1216144/140‑1 (MQ=255)                                                                                                           tCGGCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCgg                             >  1:428161/1‑140 (MQ=255)                                                                                                              gcccgcgCTCGCGCCAGCGCAGAACCGTACAGGGGTACAATCTCAGTGTTGGAACGAAGACGGTCGATCGCCGGCTCATCCGCCTGCGGGTTCAGGTCGTTTACCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGc                          <  2:315280/137‑1 (MQ=255)                                                                                                              gCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGc                          <  1:634056/140‑1 (MQ=255)                                                                                                              gCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGc                          <  1:365493/140‑1 (MQ=255)                                                                                                              gCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGc                          <  2:67125/140‑1 (MQ=255)                                                                                                              gCACGCGCTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGc                          <  1:704929/140‑1 (MQ=255)                                                                                                                   cgcTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGtt                     <  2:839742/140‑1 (MQ=255)                                                                                                                   cgcTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGtt                     <  1:1226567/140‑1 (MQ=255)                                                                                                                     cTCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGc                   <  2:1500592/140‑1 (MQ=255)                                                                                                                      tCGCGCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCa                  <  1:756568/140‑1 (MQ=255)                                                                                                                       cgcgCCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAg                 >  2:200235/1‑140 (MQ=255)                                                                                                                        gcgcCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGa                <  2:396785/140‑1 (MQ=255)                                                                                                                        gcgcCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGa                <  2:333134/140‑1 (MQ=255)                                                                                                                          gcCAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCCGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATa              <  1:1625886/140‑1 (MQ=255)                                                                                                                           ccAGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAg             <  1:1499697/140‑1 (MQ=255)                                                                                                                             aGCGCAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTc           <  2:169552/140‑1 (MQ=255)                                                                                                                              gcgcAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTcc          >  1:1294650/1‑140 (MQ=255)                                                                                                                                gcAGAAACGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTCCAg        <  1:1109640/140‑1 (MQ=255)                                                                                                                                      aCGTACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTc  <  2:1113977/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCTCGCGCCAGCGCAGAAACGCACAGCGGTACAAACTCACTGTTGGAACGAAGACGGTCGATCGCCCGCTCAACCGCCTGCGGGTTCAGGTCGTTTATCAAGCAACGCTGTACCTGCTGGCGCTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTC  >  NC_000913/1846349‑1846621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑