BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,868,816	T→C	Y637H (TAT→CAT)	yeaG →	protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,816	0	T	C	99.0%	348.8 / ‑5.0	98	Y637H (TAT→CAT)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (41/56); minor base A (1/0); total (42/56)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGG  >  NC_000913/1868679‑1868950

aTACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCAt                                                                                                                                      <  1:142762/140‑1 (MQ=255)

  aCCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCAttt                                                                                                                                    >  2:745972/1‑140 (MQ=255)

   ccGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTg                                                                                                                                   <  1:770654/140‑1 (MQ=255)

     gaggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcg                                                                                                                                 <  2:992913/140‑1 (MQ=255)

     gaggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcg                                                                                                                                 <  1:687900/140‑1 (MQ=255)

     gaggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcg                                                                                                                                 <  1:745972/140‑1 (MQ=255)

     gaggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcg                                                                                                                                 <  1:1124043/140‑1 (MQ=255)

      aggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcgc                                                                                                                                >  2:493191/1‑140 (MQ=255)

      aggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcgc                                                                                                                                >  2:926693/1‑140 (MQ=255)

      aggagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTgcgc                                                                                                                                >  2:1070675/1‑140 (MQ=255)

        gagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGcgt                                                                                                                              >  1:42678/1‑140 (MQ=255)

        gagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGcgt                                                                                                                              <  2:1500208/140‑1 (MQ=255)

        gagCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGcgt                                                                                                                              >  2:711369/1‑140 (MQ=255)

             gTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGcgtacgta                                                                                                                         >  1:189194/1‑140 (MQ=255)

              ttGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTaa                                                                                                                        >  1:924074/1‑140 (MQ=255)

                gCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAAt                                                                                                                      >  1:961244/1‑140 (MQ=255)

                 ccGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATc                                                                                                                     <  2:1066099/140‑1 (MQ=255)

                   ggTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGt                                                                                                                   >  2:337470/1‑140 (MQ=255)

                     ttATCTCGTTTAACGCTACAACGCTAACCGACGTGCCGAAGAAACACGACGACTTTGTCGACTGTATGATGGAAAAAGTCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCt                                                                                                                 >  2:1164724/1‑140 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              <  2:1210507/140‑1 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              <  2:290125/140‑1 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              <  2:357458/140‑1 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              >  1:431164/1‑140 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              <  2:42678/140‑1 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              <  2:961244/140‑1 (MQ=255)

                        tctcGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGAATTTGCGCGTACGTAAATCGTCTTaa                                                                                                              >  2:1440195/1‑140 (MQ=255)

                              ttAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAAccc                                                                                                        <  1:1200562/140‑1 (MQ=255)

                                aaCGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTg                                                                                                      <  1:1070675/140‑1 (MQ=255)

                                 aCGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTgg                                                                                                     <  1:493191/140‑1 (MQ=255)

                                 aCGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTgg                                                                                                     <  1:337470/140‑1 (MQ=255)

                                  cGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGc                                                                                                    >  2:941922/1‑140 (MQ=255)

                                  cGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGc                                                                                                    >  1:518137/1‑140 (MQ=255)

                                   gCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   <  2:878131/140‑1 (MQ=255)

                                   gCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   <  2:518137/140‑1 (MQ=255)

                                   gCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   <  1:869340/140‑1 (MQ=255)

                                   gCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   <  1:926693/140‑1 (MQ=255)

                                   gCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   <  1:129410/140‑1 (MQ=255)

                                      aaaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCgg                                                                                                >  1:1066051/1‑140 (MQ=255)

                                       aaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGt                                                                                               <  1:1142841/140‑1 (MQ=255)

                                       aaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGt                                                                                               >  2:362190/1‑140 (MQ=255)

                                        aaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   <  1:185842/135‑1 (MQ=255)

                                        aaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGcc                                                                                                   >  2:185842/1‑135 (MQ=255)

                                        aaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTa                                                                                              <  2:429184/140‑1 (MQ=255)

                                            tCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAcaca                                                                                          <  1:437203/140‑1 (MQ=255)

                                             cAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAt                                                                                         <  2:178650/140‑1 (MQ=255)

                                               aCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGt                                                                                               <  2:1462746/132‑1 (MQ=255)

                                               aCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGt                                                                                               >  1:1462746/1‑132 (MQ=255)

                                                    cgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                  <  2:150586/140‑1 (MQ=255)

                                                    cgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                  >  2:1189937/1‑140 (MQ=255)

                                                      aGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCgg                                                                                                <  1:561859/124‑1 (MQ=255)

                                                      aGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCgg                                                                                                >  2:561859/1‑124 (MQ=255)

                                                      aGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                <  2:1519526/140‑1 (MQ=255)

                                                           aagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAcc                                                                                   >  2:965335/1‑132 (MQ=255)

                                                           aagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAcc                                                                                   <  1:965335/132‑1 (MQ=255)

                                                                acacGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGa                                                                      <  1:994512/140‑1 (MQ=255)

                                                                     acgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCg                                                                 <  1:606633/140‑1 (MQ=255)

                                                                     acgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCg                                                                 >  2:606633/1‑140 (MQ=255)

                                                                          tttGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCg                                                            <  1:711369/140‑1 (MQ=255)

                                                                            tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                          >  2:550845/1‑140 (MQ=255)

                                                                            tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                          <  2:1066051/140‑1 (MQ=255)

                                                                                   cGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGTCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                   >  2:524231/1‑140 (MQ=255)

                                                                                          tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg                                            <  1:1312042/140‑1 (MQ=255)

                                                                                           ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCCTGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                           <  2:1373926/140‑1 (MQ=255)

                                                                                             aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa                                         <  2:924074/140‑1 (MQ=255)

                                                                                                    cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc                                  <  2:431164/140‑1 (MQ=255)

                                                                                                    cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc                                  <  1:1164724/140‑1 (MQ=255)

                                                                                                          ccGTAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCa                            <  1:209830/140‑1 (MQ=255)

                                                                                                             tAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAaca                                       >  1:1376538/1‑126 (MQ=255)

                                                                                                             tAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAaca                                       <  2:1376538/126‑1 (MQ=255)

                                                                                                             tAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                         >  1:66715/1‑140 (MQ=255)

                                                                                                             tAAACAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCCGAGAAGCGGCAACACAGGCGTAGCATAc                         >  1:838367/1‑140 (MQ=255)

                                                                                                               aaCAGGTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGTCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                       <  1:524231/140‑1 (MQ=255)

                                                                                                                   ggTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCC‑GTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  <  1:941922/140‑1 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                    <  1:1152293/122‑1 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                    >  2:1152293/1‑122 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                       <  1:893586/135‑1 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                       >  2:893586/1‑135 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  >  1:1573828/1‑140 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  <  2:1275809/140‑1 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  >  1:991131/1‑140 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  >  2:291627/1‑140 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  >  1:1277416/1‑140 (MQ=255)

                                                                                                                    gTGCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                  >  1:1275809/1‑140 (MQ=255)

                                                                                                                      gCGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaa                <  2:991131/140‑1 (MQ=255)

                                                                                                                       cGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa               >  2:412018/1‑140 (MQ=255)

                                                                                                                       cGTTTACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa               >  2:927417/1‑140 (MQ=255)

                                                                                                                         tttACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGACAAATg             >  2:1540097/1‑140 (MQ=255)

                                                                                                                           tACTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgta           >  1:491763/1‑140 (MQ=255)

                                                                                                                            aCTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAGCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag          >  2:1416356/1‑140 (MQ=255)

                                                                                                                             cTGTGCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCCGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagt         <  2:838367/140‑1 (MQ=255)

                                                                                                                                tgCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACg      <  2:491763/140‑1 (MQ=255)

                                                                                                                                cgCGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACg      >  2:53066/2‑140 (MQ=255)

                                                                                                                                  cGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt            >  2:1247926/1‑132 (MQ=255)

                                                                                                                                  cGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt            <  1:1247926/132‑1 (MQ=255)

                                                                                                                                  cGAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg    >  2:1079250/1‑140 (MQ=255)

                                                                                                                                   gAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg   <  2:1277416/140‑1 (MQ=255)

                                                                                                                                   gAATGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg   <  1:1179296/140‑1 (MQ=255)

                                                                                                                                    aaTGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg  <  2:1433338/140‑1 (MQ=255)

                                                                                                                                    aaTGGCATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg  <  1:1189937/140‑1 (MQ=255)

ATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGG  >  NC_000913/1868679‑1868950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`