Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,220	A→T	D34E (GAT→GAA)	gatZ ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,220	0	A	T	100.0%	204.5 / NA	58	D34E (GAT→GAA)	gatZ	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/35);  total (23/35)

TCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGT  >  NC_000913/2176083‑2176346                                                                                                                                          |                                                                                                                               tCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTTCGCGTGCTGTTGCGTTc                                                                                                                              >  1:848223/1‑140 (MQ=255) tCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTc                                                                                                                              >  1:1068271/1‑140 (MQ=255) tCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTc                                                                                                                              >  1:1241021/1‑140 (MQ=255)      tGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATg                                                                                                                         >  2:300108/1‑140 (MQ=255)          aaCCCAACTTTAGCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAg                                                                                                                     <  2:878171/140‑1 (MQ=255)             ccAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGAATGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAgcgc                                                                                                                  >  2:1254450/1‑140 (MQ=255)              cAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTTCGCGTGCTGTTGCGTTCAAATGCCAGcgcc                                                                                                                 <  2:848223/140‑1 (MQ=255)              cAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGcgcc                                                                                                                 <  2:905592/140‑1 (MQ=255)               aaCTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGcgccg                                                                                                                <  1:1185160/140‑1 (MQ=255)               aaCTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGcgccg                                                                                                                <  1:1030585/140‑1 (MQ=255)                      tCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCTTTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGcgcc                                                                                                                 <  2:751681/132‑1 (MQ=255)                      tCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGcgcc                                                                                                                 >  1:751681/1‑132 (MQ=255)                      tCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGAt                                                                                                         <  1:1566021/140‑1 (MQ=255)                         gCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAAc                                                                                                      >  2:812906/1‑140 (MQ=255)                          cAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGa                                                                                                          >  2:903178/1‑135 (MQ=255)                          cAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGa                                                                                                          <  1:903178/135‑1 (MQ=255)                           aaTCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCa                                                                                                    >  2:267349/1‑140 (MQ=255)                            aTCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCaa                                                                                                   >  1:674287/1‑140 (MQ=255)                            aTCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCaa                                                                                                   <  1:1274100/140‑1 (MQ=255)                               gTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACgg                                                                                                <  1:932842/140‑1 (MQ=255)                               gTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACgg                                                                                                <  2:674287/140‑1 (MQ=255)                               gTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACgg                                                                                                <  2:307352/140‑1 (MQ=255)                               gTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACgg                                                                                                <  1:887267/140‑1 (MQ=255)                                   aaaCAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATgg                                                                                            <  2:1383740/140‑1 (MQ=255)                                    aaCAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATggg                                                                                           <  2:1385111/140‑1 (MQ=255)                                        aaTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGa                                                                                       <  2:1068271/140‑1 (MQ=255)                                        aaTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGa                                                                                       <  1:1138647/140‑1 (MQ=255)                                         aTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACgg                                                                                                <  2:1456248/130‑1 (MQ=255)                                         aTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACgg                                                                                                >  1:1456248/1‑130 (MQ=255)                                         aTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGTAGaa                                                                                      <  1:146921/140‑1 (MQ=255)                                         aTTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGaa                                                                                      <  1:72948/140‑1 (MQ=255)                                          ttCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAAc                                                                                     <  2:1265421/140‑1 (MQ=255)                                              cgAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGAc                                                                                 <  2:83155/140‑1 (MQ=255)                                                aaaGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTg                                                                               <  2:977793/140‑1 (MQ=255)                                                 aaGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGa                                                                                       <  2:920385/131‑1 (MQ=255)                                                 aaGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGa                                                                                       >  1:920385/1‑131 (MQ=255)                                                   gTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAAc                                                                            <  1:333621/140‑1 (MQ=255)                                                   gTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAAc                                                                            <  1:812906/140‑1 (MQ=255)                                                           gtgtCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCg                                                                    >  2:1494922/1‑140 (MQ=255)                                                                  tCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGtt                                                             >  2:352191/1‑140 (MQ=255)                                                                     ggTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCAc                                                          <  1:1494922/140‑1 (MQ=255)                                                                     ggTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCAc                                                          <  2:882383/140‑1 (MQ=255)                                                                        ataACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAg                                                       >  1:856756/1‑140 (MQ=255)                                                                          aaCCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCt                                                     >  2:1230676/1‑140 (MQ=255)                                                                                    ttgaatgaCCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCggg                                           <  1:1254450/140‑1 (MQ=255)                                                                                              tGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGt                                 <  1:267349/140‑1 (MQ=255)                                                                                                gTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGttt                               >  2:1617509/1‑140 (MQ=255)                                                                                                  ttGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGGAATTAACGTTTTc                             <  2:24618/140‑1 (MQ=255)                                                                                                       gTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGtt                        >  1:1538115/1‑140 (MQ=255)                                                                                                                aTCAGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTg               >  2:1110318/1‑140 (MQ=255)                                                                                                                   aGCACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGACAGAACAGATTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTa            <  2:1513202/140‑1 (MQ=255)                                                                                                                     cACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTaaa          >  1:78336/1‑140 (MQ=255)                                                                                                                     cACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTaaa          >  1:516451/1‑140 (MQ=255)                                                                                                                     cACTTTGCGCGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTaaa          <  2:895746/140‑1 (MQ=255)                                                                                                                           gcgcGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAg    <  2:78336/140‑1 (MQ=255)                                                                                                                           gcgcGTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAg    >  1:1413251/1‑140 (MQ=255)                                                                                                                             gcgTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGt  >  2:435416/1‑140 (MQ=255)                                                                                                                             gcgTGCTGTTGCGTTCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGt  <  2:856756/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               TCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGT  >  NC_000913/2176083‑2176346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑