Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,995	A→G	intergenic (‑112/+258)	yadN ← / ← folK	putative fimbrial protein YadN/2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,995	0	A	G	100.0%	345.1 / NA	95	intergenic (‑112/+258)	yadN/folK	putative fimbrial protein YadN/2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/54);  total (41/54)

AGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  NC_000913/156858‑157131                                                                                                                                          |                                                                                                                                         aGGGCAAAACCTAATTTTTTAAACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGcc                                                                                                                                        <  1:1043423/140‑1 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      >  2:293058/1‑140 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      >  1:545829/1‑140 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      <  2:190782/140‑1 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      >  2:779681/1‑140 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      >  1:815541/1‑140 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      >  1:1338831/1‑140 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      >  1:1337585/1‑140 (MQ=255)   ggCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCa                                                                                                                                      <  2:1329316/140‑1 (MQ=255)       aaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAAtt                                                                                                                                  <  2:919879/140‑1 (MQ=255)        aaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAAttt                                                                                                                                 >  1:1372538/1‑140 (MQ=255)         aCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTa                                                                                                                                <  2:1337585/140‑1 (MQ=255)         aCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTa                                                                                                                                <  2:1600826/140‑1 (MQ=255)             aaTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAAc                                                                                                                            <  1:293058/140‑1 (MQ=255)             aaTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAAc                                                                                                                            <  1:1007863/140‑1 (MQ=255)             aaTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAAc                                                                                                                            <  1:635053/140‑1 (MQ=255)               ttttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCa                                                                                                                          <  2:818219/140‑1 (MQ=255)                 ttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCAtt                                                                                                                        <  2:622931/140‑1 (MQ=255)                 ttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCAtt                                                                                                                        <  1:779681/140‑1 (MQ=255)                 ttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCAtt                                                                                                                        <  2:304095/140‑1 (MQ=255)                   ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTGCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGt                                                                                                                      <  2:520745/140‑1 (MQ=255)                    tAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGtt                                                                                                                     <  1:804050/140‑1 (MQ=255)                    tAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGtt                                                                                                                     >  2:28173/1‑140 (MQ=255)                        cATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCAtt                                                                                                                 <  2:545829/140‑1 (MQ=255)                        cATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCAtt                                                                                                                 <  2:1419407/140‑1 (MQ=255)                          tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAg                                                                                                               <  2:1372538/140‑1 (MQ=255)                             aaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                            <  2:1338831/140‑1 (MQ=255)                               aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGc                                                                                                          <  1:495081/140‑1 (MQ=255)                                     taataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    >  2:361724/1‑140 (MQ=255)                                     taataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    >  2:292436/1‑140 (MQ=255)                                     taataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    >  1:248091/1‑140 (MQ=255)                                       ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                                  <  1:292436/140‑1 (MQ=255)                                       ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                                  <  2:1327824/140‑1 (MQ=255)                                       ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                                  <  1:1602039/140‑1 (MQ=255)                                       ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                                  <  2:420060/140‑1 (MQ=255)                                        taaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGc                                                                                                 <  2:1342832/140‑1 (MQ=255)                                           aaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACt                                                                                              >  1:942795/1‑140 (MQ=255)                                            aTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACtt                                                                                             >  2:400842/1‑140 (MQ=255)                                                cATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                         <  2:248091/140‑1 (MQ=255)                                                cATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                         <  1:1418468/140‑1 (MQ=255)                                                  ttAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAg                                                                                       >  1:1120855/1‑140 (MQ=255)                                                  ttAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTATGCACTTTAGAAg                                                                                       >  1:1385428/1‑140 (MQ=255)                                                      tCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCttt                                                                                   >  1:1507582/1‑140 (MQ=255)                                                          aCTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGaa                                                                               >  1:1313954/1‑140 (MQ=255)                                                             aCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaa                                                                            <  1:241066/140‑1 (MQ=255)                                                              cATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaa                                                                           >  1:1255149/1‑140 (MQ=255)                                                               aTGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAA‑CATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                         <  2:1498839/124‑1 (MQ=255)                                                               aTGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAA‑CATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                         >  1:1498839/1‑124 (MQ=255)                                                                    tGAAGAATGGCTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGaa                                                                               <  2:953274/130‑1 (MQ=255)                                                                    tGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGaa                                                                               >  1:953274/1‑130 (MQ=255)                                                                    tGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaattaaat                                                                     >  2:596948/1‑140 (MQ=255)                                                                    tGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTATGCACTTTAGAAGCTTTTGAACaaattaaat                                                                     <  2:1385428/140‑1 (MQ=255)                                                                         aaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACt                                                                <  2:1313954/140‑1 (MQ=255)                                                                           tGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTa                                                              >  1:376746/1‑140 (MQ=255)                                                                           tGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTa                                                              >  1:667395/1‑140 (MQ=255)                                                                             aCTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAt                                                            >  1:1566298/1‑140 (MQ=255)                                                                                  tCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCaaa                                                       >  1:865574/1‑140 (MQ=255)                                                                                     tGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAAtt                                                    <  2:667395/140‑1 (MQ=255)                                                                                     tGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAAtt                                                    <  1:1609164/140‑1 (MQ=255)                                                                                      gACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTa                                                   >  2:712235/1‑140 (MQ=255)                                                                                      gACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATCTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTa                                                   >  1:773973/1‑140 (MQ=255)                                                                                         acaAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGt                                                <  2:1047641/140‑1 (MQ=255)                                                                                                    aaaCAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCACATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                     <  2:1507582/140‑1 (MQ=255)                                                                                                      aCAAATGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATT‑AAAATTAAGTAAAAATAAGTTcac                                  <  2:709299/140‑1 (MQ=255)                                                                                                         aaTGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACaa                                >  2:1360273/1‑140 (MQ=255)                                                                                                          aTGTTAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAg                               <  1:361724/140‑1 (MQ=255)                                                                                                              tAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCa                           <  1:1547897/140‑1 (MQ=255)                                                                                                              tAGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCa                           >  2:1547897/1‑140 (MQ=255)                                                                                                               aGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCaa                          <  1:712235/140‑1 (MQ=255)                                                                                                               aGATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCaa                          <  1:596948/140‑1 (MQ=255)                                                                                                                gATATTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                         >  1:1204703/1‑140 (MQ=255)                                                                                                                 atatTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAtt                        >  2:737233/1‑140 (MQ=255)                                                                                                                   atTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTgg                      <  2:1204703/140‑1 (MQ=255)                                                                                                                   atTAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTgg                      >  1:458515/1‑140 (MQ=255)                                                                                                                    ttAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGt                     <  1:536396/140‑1 (MQ=255)                                                                                                                     tAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGtt                    <  1:1360273/140‑1 (MQ=255)                                                                                                                     tAATGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGtt                    >  1:138711/1‑140 (MQ=255)                                                                                                                      aaTGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTGACAAGTGCAATTGGTTa                   <  2:632480/140‑1 (MQ=255)                                                                                                                      aaTGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTa                   >  1:449595/1‑140 (MQ=255)                                                                                                                      aaTGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTa                   >  2:1165939/1‑140 (MQ=255)                                                                                                                      aaTGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTa                   <  2:1255149/140‑1 (MQ=255)                                                                                                                      aaTGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTa                   <  2:803057/140‑1 (MQ=255)                                                                                                                        tGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAgg                 <  2:1577877/140‑1 (MQ=255)                                                                                                                        tGAGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAgg                 <  2:1212009/140‑1 (MQ=255)                                                                                                                          aGCAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGt               >  1:150476/1‑140 (MQ=255)                                                                                                                            cAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCa                           <  1:287501/126‑1 (MQ=255)                                                                                                                            cAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCa                           >  2:287501/1‑126 (MQ=255)                                                                                                                            cAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt             <  1:1165939/140‑1 (MQ=255)                                                                                                                            cAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt             >  1:704303/1‑140 (MQ=255)                                                                                                                            cAATGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt             >  2:104316/1‑140 (MQ=255)                                                                                                                               tGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGt                     <  1:255266/129‑1 (MQ=255)                                                                                                                               tGATATTTGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGt                     >  2:255266/1‑129 (MQ=255)                                                                                                                                      tGTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGa   >  2:1606831/1‑140 (MQ=255)                                                                                                                                       gTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAt  <  2:138711/140‑1 (MQ=255)                                                                                                                                       gTTGCCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAt  <  2:113397/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  NC_000913/156858‑157131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑