Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,479,327	T→C	V42V (GTT→GTC)	dsdA →	D‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,479,327	0	T	C	100.0%	339.5 / NA	94	V42V (GTT→GTC)	dsdA	D‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/44);  total (50/44)

AAGAGACGTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATG  >  NC_000913/2479190‑2479462                                                                                                                                          |                                                                                                                                        aaGAGACGTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCgg                                                                                                                                       >  1:530103/1‑140 (MQ=255)  agagACGTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGc                                                                                                                                      >  2:355199/1‑140 (MQ=255)   gagaCGTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGcc                                                                                                                                     <  1:781867/140‑1 (MQ=255)        gTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGa                                                                                                                                  >  1:657650/1‑138 (MQ=255)        gTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGa                                                                                                                                  <  2:657650/138‑1 (MQ=255)          aCTATGGAGAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGa                                                                                                                              <  1:1435098/140‑1 (MQ=255)            tATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAAc                                                                                                                            >  2:1243365/1‑140 (MQ=255)              tGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGc                                                                                                                                      >  1:830111/1‑128 (MQ=255)              tGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGc                                                                                                                                      <  2:830111/128‑1 (MQ=255)              tGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAg                                                                                                                          >  1:1344638/1‑140 (MQ=255)              tGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAg                                                                                                                          >  1:1426378/1‑140 (MQ=255)              tGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAg                                                                                                                          >  1:1377405/1‑140 (MQ=255)              tGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAg                                                                                                                          >  1:831459/1‑140 (MQ=255)               ggAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAgg                                                                                                                         <  1:1502361/140‑1 (MQ=255)                gAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGa                                                                                                                        >  1:4191/1‑140 (MQ=255)                    aCGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGtt                                                                                                                    <  2:348896/140‑1 (MQ=255)                    aCGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGCCCTGACCGAACAGGATGtt                                                                                                                    <  2:1155666/140‑1 (MQ=255)                     cGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTc                                                                                                                   >  1:466843/1‑140 (MQ=255)                      gCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCa                                                                                                                  >  1:533725/1‑140 (MQ=255)                        tAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAgg                                                                                                                <  2:1037566/140‑1 (MQ=255)                        tAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAgg                                                                                                                >  1:1185130/1‑140 (MQ=255)                         aaaaTGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGa                                                                                                               <  2:208662/140‑1 (MQ=255)                               aaCTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGtt                                                                                                                    >  2:560488/1‑129 (MQ=255)                               aaCTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGtt                                                                                                                    <  1:560488/129‑1 (MQ=255)                                 cTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGTAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGt                                                                                                                     >  2:1496707/1‑126 (MQ=255)                                 cTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGTAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGt                                                                                                                     <  1:1496707/126‑1 (MQ=255)                                    gCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATgcgc                                                                                                    <  2:376585/140‑1 (MQ=255)                                           gCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATc                                                                                             <  1:1240646/140‑1 (MQ=255)                                            cccAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATcc                                                                                            >  2:670654/1‑140 (MQ=255)                                             ccAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATccc                                                                                           <  2:402867/140‑1 (MQ=255)                                             ccAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATccc                                                                                           <  2:533725/140‑1 (MQ=255)                                                 tATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGttt                                                                                       <  2:4191/140‑1 (MQ=255)                                                 tATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGttt                                                                                       <  2:1271359/140‑1 (MQ=255)                                                  aTCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGtttt                                                                                      <  2:1377405/140‑1 (MQ=255)                                                     cGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCa                                                                                   <  2:1185130/140‑1 (MQ=255)                                                      gTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCAc                                                                                  >  2:1470040/1‑140 (MQ=255)                                                       ttGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCAcc                                                                                 >  2:279715/1‑140 (MQ=255)                                                         ggTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCt                                                                               >  1:637515/1‑140 (MQ=255)                                                           tAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACTTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTAt                                                                             >  2:240896/1‑140 (MQ=255)                                                           tAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATc                                                                                             >  1:86177/1‑124 (MQ=255)                                                           tAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATc                                                                                             <  2:86177/124‑1 (MQ=255)                                                           tAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTAt                                                                             >  1:396728/1‑140 (MQ=255)                                                           tAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTAt                                                                             >  1:966010/1‑140 (MQ=255)                                                                    tGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCaaaa                                                                    >  2:455059/1‑140 (MQ=255)                                                                    tGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCaaaa                                                                    >  2:153030/1‑140 (MQ=255)                                                                      gTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGc                                                                  >  2:778384/1‑140 (MQ=255)                                                                       ttGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCa                                                                 <  1:605059/140‑1 (MQ=255)                                                                        tGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCAt                                                                <  2:1539060/140‑1 (MQ=255)                                                                           tctTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTc                                                             >  2:175171/1‑140 (MQ=255)                                                                           tctTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTc                                                             >  1:788502/1‑140 (MQ=255)                                                                           tctTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTc                                                             >  1:1030945/1‑140 (MQ=255)                                                                             ttAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCt                                                           >  2:226252/1‑140 (MQ=255)                                                                              tAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTg                                                          <  1:226252/140‑1 (MQ=255)                                                                              tAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTg                                                          <  2:966010/140‑1 (MQ=255)                                                                                aaGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGaa                                                        >  2:1303915/1‑140 (MQ=255)                                                                                 aGAAACCACCTGGTTCAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGaaa                                                       <  2:1530924/140‑1 (MQ=255)                                                                                    aaCCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAActc                                                    >  2:1499746/1‑139 (MQ=255)                                                                                      ccaccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAActgct                                                  >  2:1064427/1‑140 (MQ=255)                                                                                       caccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAActgctg                                                 <  2:530103/140‑1 (MQ=255)                                                                                       caccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAActgctg                                                 >  2:1057078/1‑140 (MQ=255)                                                                                         ccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGTTTTACCTTATGCCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGcc                                               >  1:764598/1‑140 (MQ=255)                                                                                         ccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGcc                                               >  2:626137/1‑140 (MQ=255)                                                                                         ccTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGcc                                               >  1:671837/1‑140 (MQ=255)                                                                                            ggTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACt                                            <  1:1269700/140‑1 (MQ=255)                                                                                            ggTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACt                                            <  1:279715/140‑1 (MQ=255)                                                                                             gTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTG‑CACTgg                                          >  1:908678/1‑140 (MQ=255)                                                                                              tttAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgg                                          <  2:1426378/140‑1 (MQ=255)                                                                                              tttAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgg                                          <  2:1030945/140‑1 (MQ=255)                                                                                              tttAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgg                                          <  2:1344638/140‑1 (MQ=255)                                                                                                   tCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGa                                     >  2:146821/1‑140 (MQ=255)                                                                                                       ggCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattat                                 <  1:355199/140‑1 (MQ=255)                                                                                                       ggCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattat                                 >  2:900327/1‑140 (MQ=255)                                                                                                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGGACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTAGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattat                                 >  2:1116541/1‑139 (MQ=255)                                                                                                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgg                                          <  2:1320911/130‑1 (MQ=255)                                                                                                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTgg                                          >  1:1320911/1‑130 (MQ=255)                                                                                                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattatt                                >  2:980727/1‑140 (MQ=255)                                                                                                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattatt                                >  2:1233951/1‑140 (MQ=255)                                                                                                        gCACGACCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGattat                                 <  1:1116541/139‑1 (MQ=255)                                                                                                             aCCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACt                                            >  1:922258/1‑123 (MQ=255)                                                                                                             aCCTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACt                                            <  2:922258/123‑1 (MQ=255)                                                                                                              ccTCATTGGCTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCa                          <  1:1233951/140‑1 (MQ=255)                                                                                                                       cTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGtt                 <  1:153030/140‑1 (MQ=255)                                                                                                                       cTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGtt                 <  1:1470040/140‑1 (MQ=255)                                                                                                                       cTGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGtt                 <  1:240896/140‑1 (MQ=255)                                                                                                                        tGAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTg                <  1:1243365/140‑1 (MQ=255)                                                                                                                         gAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAAt                            <  2:784453/127‑1 (MQ=255)                                                                                                                         gAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAAt                            >  1:784453/1‑127 (MQ=255)                                                                                                                         gAAGGTTTACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGc               <  2:396728/140‑1 (MQ=255)                                                                                                                                tACCTTATGTCGGCCTGACCGGACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCa        >  2:1150512/1‑140 (MQ=255)                                                                                                                                tACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCa        >  1:1023896/1‑140 (MQ=255)                                                                                                                                tACCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCa        >  1:54901/1‑140 (MQ=255)                                                                                                                                 aCCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAg       <  2:1023896/140‑1 (MQ=255)                                                                                                                                 aCCTTATGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAg       <  1:1057078/140‑1 (MQ=255)                                                                                                                                      aTGTCGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATg  >  2:536091/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AAGAGACGTACTATGGAAAACGCTAAAATGAACTCGCTCATCGCCCAGTATCCGTTGGTAAAGGATCTGGTTGCTCTTAAAGAAACCACCTGGTTTAATCCTGGCACGACCTCATTGGCTGAAGGTTTACCTTATGTTGGCCTGACCGAACAGGATGTTCAGGACGCCCATGCGCGCTTATCCCGTTTTGCACCCTATCTGGCAAAAGCATTTCCTGAAACTGCTGCCACTGGGGGGATTATTGAATCAGAACTGGTTGCCATTCCAGCTATG  >  NC_000913/2479190‑2479462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑