Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,530,730	T→C	E168E (GAA→GAG)	zipA ←	cell division protein ZipA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,730	0	T	C	100.0%	247.9 / NA	70	E168E (GAA→GAG)	zipA	cell division protein ZipA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/26);  total (44/26)

TTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCA  >  NC_000913/2530593‑2530860                                                                                                                                          |                                                                                                                                   ttAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTc                                                                                                                                  >  1:1570874/1‑140 (MQ=255)         cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAgctg                                                                                                                          >  2:1592696/1‑137 (MQ=255)           gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTg                                                                                                                                >  1:466909/1‑132 (MQ=255)           gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTg                                                                                                                                <  2:466909/132‑1 (MQ=255)             tCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGaa                                                                                                                      <  2:1570874/140‑1 (MQ=255)                ccGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTCCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGc                                                                                                                   <  1:786257/140‑1 (MQ=255)                      aGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCtgttg                                                                                                             >  2:1380543/1‑140 (MQ=255)                      aGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCtgttg                                                                                                             >  2:1085600/1‑140 (MQ=255)                           gCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCg                                                                                                        >  1:1539699/1‑140 (MQ=255)                             tACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGt                                                                                                      >  1:407608/1‑140 (MQ=255)                             tACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGt                                                                                                      >  1:709974/1‑140 (MQ=255)                              aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCA‑GCTGGAAAGCCTGTTGTGCCGGTTg                                                                                                    <  1:1592696/140‑1 (MQ=255)                               ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTg                                                                                                    <  2:727292/140‑1 (MQ=255)                                cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                                                   >  2:1293600/1‑140 (MQ=255)                                cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                                                   >  2:169993/1‑140 (MQ=255)                                 gTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                                                  <  2:960950/140‑1 (MQ=255)                                     tGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGc                                                                                              >  1:558445/1‑140 (MQ=255)                                     tGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTACAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                                                  <  1:79077/136‑1 (MQ=255)                                     tGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTACAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                                                  >  2:79077/1‑136 (MQ=255)                                       cgccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                                            >  2:725601/1‑140 (MQ=255)                                       cgccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                                            >  1:1453079/1‑140 (MQ=255)                                       cgccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCT‑AGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                                                                           >  2:1256701/1‑140 (MQ=255)                                         ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGaa                                                                                          >  2:1463532/1‑140 (MQ=255)                                         ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGaa                                                                                          >  1:909415/1‑140 (MQ=255)                                             gACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGca                                                                                      <  1:725601/140‑1 (MQ=255)                                                    aTGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                                               <  1:748164/140‑1 (MQ=255)                                                              ccGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGgc                                                                     <  2:709974/140‑1 (MQ=255)                                                                 cttcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGcgcg                                                                  >  1:569409/1‑140 (MQ=255)                                                                   tcttTGCGCTTCGGTTTATCCATAACTGGGGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAGGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                                                >  1:372972/1‑140 (MQ=255)                                                                   tcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                                                <  2:909415/140‑1 (MQ=255)                                                                   tcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                                                >  2:294623/1‑140 (MQ=255)                                                                       tGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgt                                                            >  2:1482180/1‑140 (MQ=255)                                                                       tGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCT‑AGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgtg                                                           <  1:1256701/140‑1 (MQ=255)                                                                        gcgcTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgtg                                                           <  1:1085600/140‑1 (MQ=255)                                                                            ttCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGa                                                       >  2:1542954/1‑140 (MQ=255)                                                                              cGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaaa                                                     >  2:39017/1‑140 (MQ=255)                                                                                   tATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                                                <  1:294623/140‑1 (MQ=255)                                                                                         tAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                                          <  1:1463532/140‑1 (MQ=255)                                                                                              ggAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                                     <  1:1044562/140‑1 (MQ=255)                                                                                               gAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGc                                    >  2:240952/1‑140 (MQ=255)                                                                                               gAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACATGTGGCGAAACTGGCTGCTGCAACGGc                                    >  1:119088/1‑140 (MQ=255)                                                                                                aGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCt                                   >  1:974388/1‑140 (MQ=255)                                                                                                aGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCAGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCt                                   >  2:1186548/1‑140 (MQ=255)                                                                                                   aGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt                                <  1:1542954/140‑1 (MQ=255)                                                                                                   aGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt                                <  1:1380543/140‑1 (MQ=255)                                                                                                   aGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt                                <  1:169993/140‑1 (MQ=255)                                                                                                    ggTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                                          >  2:1222029/1‑129 (MQ=255)                                                                                                    ggTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                                          <  1:1222029/129‑1 (MQ=255)                                                                                                     gTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                                     >  1:20101/1‑133 (MQ=255)                                                                                                     gTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                                     <  2:20101/133‑1 (MQ=255)                                                                                                     gTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCa                              >  1:1233289/1‑140 (MQ=255)                                                                                                       tCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAgg                            >  1:1310316/1‑140 (MQ=255)                                                                                                         cGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCt                          <  2:407608/140‑1 (MQ=255)                                                                                                               aggctcaggctGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCt                    <  1:39017/140‑1 (MQ=255)                                                                                                               aggctcaggctGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCt                    >  1:1226222/1‑140 (MQ=255)                                                                                                                 gctcaggctGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGa                  >  1:362482/1‑140 (MQ=255)                                                                                                                           tggtgCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGgct        >  1:444817/1‑140 (MQ=255)                                                                                                                           tggtgCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGgct        >  1:1087445/1‑140 (MQ=255)                                                                                                                           tggtgCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGgct        >  2:634755/1‑140 (MQ=255)                                                                                                                           tggtgCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGgct        >  2:691228/1‑140 (MQ=255)                                                                                                                             gtgCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGgctgc      <  2:341232/140‑1 (MQ=255)                                                                                                                             gtgCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGgctgc      >  1:341232/1‑140 (MQ=255)                                                                                                                                 cGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATagg              >  2:1622918/1‑128 (MQ=255)                                                                                                                                 cGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATagg              <  1:1622918/128‑1 (MQ=255)                                                                                                                                 cGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCa  >  1:828433/1‑140 (MQ=255)                                                                                                                                 cGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCa  >  1:765137/1‑140 (MQ=255)                                                                                                                                 cGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCa  >  1:9701/1‑140 (MQ=255)                                                                                                                                 cGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCa  >  2:1047430/1‑140 (MQ=255)                                                                                                                                     aCGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATaggca            <  2:610734/126‑1 (MQ=255)                                                                                                                                     aCGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATaggca            >  1:610734/1‑126 (MQ=255)                                                                                                                                          |                                                                                                                                   TTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCA  >  NC_000913/2530593‑2530860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑