Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,539,061	T→C	T175A (ACG→GCG)	cysM ←	cysteine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,539,061	0	T	C	100.0%	244.1 / NA	68	T175A (ACG→GCG)	cysM	cysteine synthase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/32);  total (36/32)

CCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGC  >  NC_000913/2538928‑2539196                                                                                                                                      |                                                                                                                                         cccGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCAt                                                                                                                                    >  2:1314881/1‑140 (MQ=255)          tatTCCGTAGGCCAGCGGCGACTGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGAc                                                                                                                           <  2:1478732/140‑1 (MQ=255)           atTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACa                                                                                                                          >  1:385500/1‑140 (MQ=255)              ccGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACaaaa                                                                                                                       <  2:585123/140‑1 (MQ=255)               cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAAt                                                                                                                      <  2:470496/140‑1 (MQ=255)               cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAAt                                                                                                                      >  2:148903/1‑140 (MQ=255)                gTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGga                                                                                                                              >  2:318224/1‑131 (MQ=255)                gTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGga                                                                                                                              <  1:318224/131‑1 (MQ=255)                 tAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGa                                                                                                                    >  1:522871/1‑140 (MQ=255)                 tAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGa                                                                                                                    >  2:245215/1‑140 (MQ=255)                   ggCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGt                                                                                                                  >  2:1369687/1‑140 (MQ=255)                    gCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCTGTGATAGTGCCGGTCGCCCCCATGCTGGAGACa                                                                                                                          >  2:1516913/1‑131 (MQ=255)                    gCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACa                                                                                                                          <  1:1516913/131‑1 (MQ=255)                    gCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTg                                                                                                                 >  2:1036777/1‑140 (MQ=255)                    gCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTg                                                                                                                 <  2:522871/140‑1 (MQ=255)                      cAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAt                                                                                                               <  2:600671/140‑1 (MQ=255)                                  ccGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGt                                                                                                  >  2:827868/1‑140 (MQ=255)                                    gggAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGttt                                                                                                <  1:245215/140‑1 (MQ=255)                                          gctgctgcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGcc                                                                                          >  2:608601/1‑140 (MQ=255)                                           ctgctgcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑Gcgcc                                                                                                         >  1:898864/1‑124 (MQ=255)                                           ctgctgcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑Gcgcc                                                                                                         <  2:898864/124‑1 (MQ=255)                                           ctgctgcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCa                                                                                         >  2:1100511/1‑140 (MQ=255)                                                 cccTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGGGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTc                                                                                   <  1:1369687/140‑1 (MQ=255)                                                   ctctTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCg                                                                                 >  1:866865/1‑140 (MQ=255)                                                    tctTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCgg                                                                                <  1:242790/140‑1 (MQ=255)                                                      ttCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGcc                                                                              <  2:23373/140‑1 (MQ=255)                                                      ttCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGATTTGCTGCCAGATTTc                                                                                   <  1:760060/135‑1 (MQ=255)                                                      ttCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGATTTGCTGCCAGATTTc                                                                                   >  2:760060/1‑135 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAg                                                                           <  1:654642/140‑1 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAg                                                                           <  1:827868/140‑1 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAg                                                                           <  1:549980/140‑1 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAg                                                                           <  2:852156/140‑1 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAg                                                                           <  1:385392/140‑1 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTACCAGATTTCCGGCCCAg                                                                           <  1:1314881/140‑1 (MQ=255)                                                         cGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGAATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAg                                                                           <  2:1498319/140‑1 (MQ=255)                                                           gTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAgtg                                                                         >  1:782213/1‑140 (MQ=255)                                                           gTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGAGAGTGCCGGTCGCCCCCAGGTTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAgtg                                                                         <  2:214590/140‑1 (MQ=255)                                                            ttGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAgtgg                                                                        <  2:586436/140‑1 (MQ=255)                                                                     gACAATGGTCCCCGGTTTGGATGGTCCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCGGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAAt                                                               <  2:1439964/140‑1 (MQ=255)                                                                        aaTGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATgcg                                                            <  2:866865/140‑1 (MQ=255)                                                                           ggTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCAt                                                         <  2:782213/140‑1 (MQ=255)                                                                            gTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAAt                                                               <  2:514794/133‑1 (MQ=255)                                                                            gTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAAt                                                               >  1:514794/1‑133 (MQ=255)                                                                            gTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATa                                                        <  1:1036777/140‑1 (MQ=255)                                                                               cctggTTTGAATTTTTCGCGCATAAAGCGTGAGACGCCGGTGATAGGCCCGGTCCCCCCCATGCGGGAGAAAAAATGAG‑GATCGGGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAgg                                                     <  2:385500/137‑1 (MQ=255)                                                                                 cGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGt                                                   >  2:94288/1‑140 (MQ=255)                                                                                 cGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGt                                                   >  1:146129/1‑140 (MQ=255)                                                                                      tGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCg                                              >  2:568769/1‑140 (MQ=255)                                                                                             ttCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCgg                                             <  1:1022902/134‑1 (MQ=255)                                                                                             ttCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCgg                                             >  2:1022902/1‑134 (MQ=255)                                                                                             ttCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGattat                                       >  1:684190/1‑140 (MQ=255)                                                                                             ttCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGattat                                       >  2:1368943/1‑140 (MQ=255)                                                                                                   cATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACt                                 >  2:271833/1‑140 (MQ=255)                                                                                                     tAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGa                               <  1:259080/140‑1 (MQ=255)                                                                                                       aaGCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATc                             >  2:580092/1‑140 (MQ=255)                                                                                                         gCGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGa                           <  2:357339/140‑1 (MQ=255)                                                                                                          cGTGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGag                          <  1:786472/140‑1 (MQ=255)                                                                                                            tGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGaa                                   <  2:659867/129‑1 (MQ=255)                                                                                                            tGAGACGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGaa                                   >  1:659867/1‑129 (MQ=255)                                                                                                                aCGCCGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCtt                    >  1:685481/1‑140 (MQ=255)                                                                                                                   ccGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTcc                 >  1:673495/1‑140 (MQ=255)                                                                                                                    cGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCt                >  1:633813/1‑140 (MQ=255)                                                                                                                    cGGTGATAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCt                >  2:346548/1‑140 (MQ=255)                                                                                                                     ggTGATAGTGCCGGTCGCCCCCATGCGGGAGACAAAATTAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCtt               <  2:852638/140‑1 (MQ=255)                                                                                                                         aTAGTGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGcc           >  2:919538/1‑140 (MQ=255)                                                                                                                             tGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGa       <  1:500996/140‑1 (MQ=255)                                                                                                                             tGCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGa       >  2:1569814/1‑140 (MQ=255)                                                                                                                              gCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGAt      >  1:717204/1‑140 (MQ=255)                                                                                                                              gCCGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGAt      <  2:50940/140‑1 (MQ=255)                                                                                                                                cGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTc    >  1:507077/1‑140 (MQ=255)                                                                                                                                cGGTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTc    >  1:931242/1‑140 (MQ=255)                                                                                                                                 ggTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCg   >  1:426812/1‑140 (MQ=255)                                                                                                                                 ggTCGCCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTCATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCg   >  1:522054/1‑140 (MQ=255)                                                                                                                                  gtcgccCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGc  >  2:199278/1‑140 (MQ=255)                                                                                                                                  gtcgccCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGc  >  2:885839/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCATGCTGGAGACAAAATGAGTGAT‑GCGCCCGCCGGTTTGCTGCCAGATTTCCGGCCCAGTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGC  >  NC_000913/2538928‑2539196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑