Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,709,734	T→C	P93P (CCA→CCG)	rpoE ←	RNA polymerase sigma E factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,709,734	0	T	C	100.0%	320.5 / NA	89	P93P (CCA→CCG)	rpoE	RNA polymerase sigma E factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/46);  total (43/46)

GGGACTCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGTGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTACC  >  NC_000913/2709601‑2709866                                                                                                                                      |                                                                                                                                     gggaCTCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGgacga                                                                                                                                <  1:1026904/140‑1 (MQ=255) gggaCTCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGgacga                                                                                                                                <  2:848233/140‑1 (MQ=255)   gaCTCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGacgacg                                                                                                                              >  1:1195704/1‑140 (MQ=255)     cTCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGcc                                                                                                                            >  1:962566/1‑140 (MQ=255)      tCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGccc                                                                                                                           <  2:645803/140‑1 (MQ=255)      tCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGccc                                                                                                                           <  2:102761/140‑1 (MQ=255)      tCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGccc                                                                                                                           <  2:689158/140‑1 (MQ=255)            gTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGc                                                                                                                     >  1:1309669/1‑140 (MQ=255)             ttCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCa                                                                                                                    >  2:1243201/1‑140 (MQ=255)             ttCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCa                                                                                                                    >  2:1347453/1‑140 (MQ=255)             ttCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCa                                                                                                                    >  1:1095395/1‑140 (MQ=255)             ttCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCa                                                                                                                    >  1:1410843/1‑140 (MQ=255)             ttCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCa                                                                                                                    >  1:1488599/1‑140 (MQ=255)             ttCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTC‑GCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCaa                                                                                                                   <  2:331975/140‑1 (MQ=255)               cGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAAc                                                                                                                  >  1:636149/1‑140 (MQ=255)               cGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAAc                                                                                                                  >  2:1041793/1‑140 (MQ=255)                ggAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAAcc                                                                                                                 >  1:919307/1‑140 (MQ=255)                  aaaaCTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAg                                                                                                               >  2:543386/1‑140 (MQ=255)                    aaCTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGt                                                                                                             <  1:1486856/140‑1 (MQ=255)                          cTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAAtttt                                                                                                       <  2:1309669/140‑1 (MQ=255)                            gTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTc                                                                                                     >  2:1414141/1‑140 (MQ=255)                              ctcAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGc                                                                                                   <  1:1243201/140‑1 (MQ=255)                              ctcAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGc                                                                                                   <  2:1117771/140‑1 (MQ=255)                              ctcAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGc                                                                                                   <  1:1432763/140‑1 (MQ=255)                              ctcAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGc                                                                                                   <  1:1347453/140‑1 (MQ=255)                                     cttctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCt                                                                                                  <  1:930077/134‑1 (MQ=255)                                     cttctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCt                                                                                                  >  2:930077/1‑134 (MQ=255)                                       tctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCt                                                                                                  <  2:1230505/132‑1 (MQ=255)                                       tctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCt                                                                                                  >  1:1230505/1‑132 (MQ=255)                                       tctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTAc                                                                                          <  2:636149/140‑1 (MQ=255)                                       tctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTAc                                                                                          >  2:978148/1‑140 (MQ=255)                                       tctGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTAc                                                                                          >  1:1329962/1‑140 (MQ=255)                                         tGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAg                                                                                        <  2:919307/140‑1 (MQ=255)                                           acaacaTTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCa                                                                                      >  1:891674/1‑140 (MQ=255)                                             aacaTTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAAt                                                                                    <  1:1040680/140‑1 (MQ=255)                                             aacaTTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAAt                                                                                    >  1:1164285/1‑140 (MQ=255)                                                aTTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCg                                                                                 >  2:474277/1‑140 (MQ=255)                                                  tAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGAt                                                                               >  1:827461/1‑140 (MQ=255)                                                  tAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCACCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGAt                                                                               >  1:301941/1‑140 (MQ=255)                                                   aaGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATa                                                                              <  2:1195704/140‑1 (MQ=255)                                                   aaGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATa                                                                              <  1:474277/140‑1 (MQ=255)                                                   aaGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATa                                                                              <  1:1404219/140‑1 (MQ=255)                                                     gTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACa                                                                            >  2:1129399/1‑140 (MQ=255)                                                     gTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACa                                                                            >  2:552216/1‑140 (MQ=255)                                                       tctcAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGc                                                                          <  1:978148/140‑1 (MQ=255)                                                       tctcAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGc                                                                          <  1:36284/140‑1 (MQ=255)                                                       tctcAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGc                                                                          <  1:698699/140‑1 (MQ=255)                                                         tcAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCa                                                                        >  1:998932/1‑140 (MQ=255)                                                         tcAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCa                                                                        >  2:841687/1‑140 (MQ=255)                                                          cAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCAt                                                                       <  1:543386/140‑1 (MQ=255)                                                           aGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAg                                                                                        >  1:994470/1‑122 (MQ=255)                                                           aGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGc                                                                          <  1:30363/136‑1 (MQ=255)                                                           aGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGc                                                                          >  2:30363/1‑136 (MQ=255)                                                           aGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATg                                                                      >  2:843131/1‑140 (MQ=255)                                                           aGGGTTCGAAATCTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAg                                                                                        <  2:994470/122‑1 (MQ=255)                                                               ttCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGtata                                                                  <  1:687131/140‑1 (MQ=255)                                                                tCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATaa                                                                 >  1:872836/1‑140 (MQ=255)                                                                tCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATaa                                                                 >  1:25133/1‑140 (MQ=255)                                                                 cGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATaaa                                                                >  1:1409973/1‑140 (MQ=255)                                                                       ttctttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAgcgc                                                          >  1:629802/1‑140 (MQ=255)                                                                       ttctttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAgcgc                                                          >  1:387318/1‑140 (MQ=255)                                                                        tctttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCt                                                         <  2:1020346/140‑1 (MQ=255)                                                                          tttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTAt                                                       <  1:1414141/140‑1 (MQ=255)                                                                          tttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTAt                                                       <  1:552216/140‑1 (MQ=255)                                                                          tttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTAt                                                       <  1:1129399/140‑1 (MQ=255)                                                                          tttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTAt                                                       <  2:891674/140‑1 (MQ=255)                                                                          tttcAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTAt                                                       <  2:1410843/140‑1 (MQ=255)                                                                                cgcgCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTcccc                                                 <  1:833619/140‑1 (MQ=255)                                                                                              cGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAgc                                   <  2:25133/140‑1 (MQ=255)                                                                                              cGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAgc                                   <  1:809237/140‑1 (MQ=255)                                                                                                    tttCAGCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGa                             <  1:1041793/140‑1 (MQ=255)                                                                                                         gCTTCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGc                        <  2:1409973/140‑1 (MQ=255)                                                                                                            tCAATGGCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCttt                     >  2:719191/1‑140 (MQ=255)                                                                                                                  gCATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATaaa               <  2:827461/140‑1 (MQ=255)                                                                                                                   cATCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATaaaa              <  1:457745/140‑1 (MQ=255)                                                                                                                    aTCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAg             <  2:1095395/140‑1 (MQ=255)                                                                                                                     tCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGc            >  2:1069237/1‑140 (MQ=255)                                                                                                                     tCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGc            >  2:1377616/1‑140 (MQ=255)                                                                                                                     tCCACATCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGc            >  2:203796/1‑140 (MQ=255)                                                                                                                        acaTCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGcttc         <  1:284345/140‑1 (MQ=255)                                                                                                                         caTCACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGcttct        <  2:595811/140‑1 (MQ=255)                                                                                                                            cACTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGt     >  1:1388874/1‑140 (MQ=255)                                                                                                                              cTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTAc   <  2:1488599/140‑1 (MQ=255)                                                                                                                              cTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTAc   >  2:764858/1‑140 (MQ=255)                                                                                                                              cTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTAc   >  2:395895/1‑140 (MQ=255)                                                                                                                              cTGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTAc   <  2:387318/140‑1 (MQ=255)                                                                                                                               tGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGcttc         >  1:773765/1‑133 (MQ=255)                                                                                                                               tGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGcttc         <  2:773765/133‑1 (MQ=255)                                                                                                                               tGGAAGGCGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTAcc  >  1:1270025/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     GGGACTCAATAGTTCGGAAAACTATCTGTCTCAGTTCTTCTGACAACATTAAGTTCTCAGGGTTCGAAATTTCTTTCAACGCGCCGCCACTTTCGAAGTTTTCAGCTTCAATGGCATCCACATCACTGGAAGGTGGACGACGCCCCTGAGCAACCAGGTAATTTTTCGCTGTATTTACAGCAATCCGATACAGCCATGTATAAAAAGCGCTATCTCCCCGGAACGAATCCAGCGCACGATAGGCTTTAATAAAAGCTTCTTGTACC  >  NC_000913/2709601‑2709866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑