Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,806,550	C→T	R181C (CGT→TGT)	proW →	glycine betaine ABC transporter membrane subunit ProW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,806,550	0	C	T	100.0%	275.8 / NA	77	R181C (CGT→TGT)	proW	glycine betaine ABC transporter membrane subunit ProW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/41);  total (36/41)

GCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTCGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGATCATCTTTGCTCTGCCGCCGATTATCCGTCTGA  >  NC_000913/2806419‑2806685                                                                                                                                    |                                                                                                                                        gcgcAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTg                                                                                                                                 >  1:1470826/1‑140 (MQ=255)  cgcAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGc                                                                                                                                >  2:1556177/1‑140 (MQ=255)   gcAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCTTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCt                                                                                                                               <  2:1476790/140‑1 (MQ=255)     aaTCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTg                                                                                                                             >  1:616209/1‑140 (MQ=255)       tCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGAt                                                                                                                           <  2:452491/140‑1 (MQ=255)       tCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGAt                                                                                                                           <  1:1301577/140‑1 (MQ=255)            gCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCAt                                                                                                                      >  1:670515/1‑140 (MQ=255)             ccTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATg                                                                                                                     <  2:1287941/140‑1 (MQ=255)                      aGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACg                                                                                                            <  1:1203730/140‑1 (MQ=255)                      aGGCAATGGTGACTCTGGCGCGGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACg                                                                                                            >  2:1149865/1‑140 (MQ=255)                        gCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGcc                                                                                                          <  2:1536924/140‑1 (MQ=255)                           aTGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGc                                                                                                       <  2:120065/140‑1 (MQ=255)                           aTGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGc                                                                                                       <  2:1470826/140‑1 (MQ=255)                            tGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCg                                                                                                      <  1:692649/140‑1 (MQ=255)                                 aCTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCgtttgt                                                                                                 >  1:318563/1‑140 (MQ=255)                                  ctctGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCgtttgtt                                                                                                >  1:1574081/1‑140 (MQ=255)                                  ctctGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCgtttgtt                                                                                                >  1:636277/1‑140 (MQ=255)                                  ctctGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCgtttgtt                                                                                                >  2:18037/1‑140 (MQ=255)                                      ggCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATc                                                                                            >  2:1226405/1‑140 (MQ=255)                                      ggCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATc                                                                                            <  1:649470/140‑1 (MQ=255)                                      ggCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATc                                                                                            >  2:649470/1‑140 (MQ=255)                                      ggCGCTGGGGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATc                                                                                            >  2:269279/1‑140 (MQ=255)                                        cgcTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTg                                                                                          <  1:1160340/140‑1 (MQ=255)                                         gcTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTgg                                                                                         >  2:1302408/1‑140 (MQ=255)                                         gcTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTgg                                                                                         >  1:1141979/1‑140 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     <  1:1149865/140‑1 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     >  1:832281/1‑140 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     <  1:659042/140‑1 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     >  2:559866/1‑140 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     >  1:876803/1‑140 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     <  2:553323/140‑1 (MQ=255)                                             gtgtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     >  1:235180/1‑140 (MQ=255)                                               gtTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCaa                                                                                   <  1:18037/140‑1 (MQ=255)                                                    ccGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTc                                                                              <  2:318563/140‑1 (MQ=255)                                                     cGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCa                                                                             <  1:850144/140‑1 (MQ=255)                                                     cGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCa                                                                             <  1:1240085/140‑1 (MQ=255)                                                       cccTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     <  1:1170748/130‑1 (MQ=255)                                                       cccTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGcc                                                                                     >  2:1170748/1‑130 (MQ=255)                                                       cccTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCa                                                                             >  2:750421/1‑138 (MQ=255)                                                       cccTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCa                                                                             <  1:750421/138‑1 (MQ=255)                                                       cccTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATg                                                                           <  1:802604/140‑1 (MQ=255)                                                                    tATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATg                                                                           >  1:1304353/1‑127 (MQ=255)                                                                    tATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATg                                                                           <  2:1304353/127‑1 (MQ=255)                                                                    tATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCg                                                              >  1:11615/1‑140 (MQ=255)                                                                        gTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTaa                                                          <  1:1226405/140‑1 (MQ=255)                                                                         tCATCGGTTTGCCGTTGGGGATATGGCTGGCGTGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAAc                                                         >  1:1271519/1‑140 (MQ=255)                                                                           aTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTAt                                                                       <  2:1190000/124‑1 (MQ=255)                                                                           aTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTAt                                                                       >  1:1190000/1‑124 (MQ=255)                                                                           aTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTg                                                                    <  2:938436/127‑1 (MQ=255)                                                                           aTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTg                                                                    >  1:938436/1‑127 (MQ=255)                                                                           aTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGt                                                       >  1:68276/1‑140 (MQ=255)                                                                           aTCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGt                                                       >  2:1625789/1‑140 (MQ=255)                                                                            tCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTa                                                           <  2:380058/135‑1 (MQ=255)                                                                            tCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTa                                                           >  1:380058/1‑135 (MQ=255)                                                                                 ttGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCggg                                                 <  2:670515/140‑1 (MQ=255)                                                                                 ttGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCggg                                                 <  1:1625789/140‑1 (MQ=255)                                                                                 ttGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCggg                                                 <  1:1222405/140‑1 (MQ=255)                                                                                 ttGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCggg                                                 <  2:1141979/140‑1 (MQ=255)                                                                                  tGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGc                                                >  1:59196/1‑140 (MQ=255)                                                                                  tGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGc                                                >  1:427969/1‑140 (MQ=255)                                                                                  tGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGc                                                >  1:364966/1‑140 (MQ=255)                                                                                   gCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGTCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCg                                               >  1:1485264/1‑140 (MQ=255)                                                                                   gCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCg                                               <  2:11615/140‑1 (MQ=255)                                                                                   gCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCg                                               >  1:1552394/1‑140 (MQ=255)                                                                                     cGTTGGGGATATGGCTGGCGTGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCgtg                                             <  2:1271519/140‑1 (MQ=255)                                                                                         ggggATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCgtggtgg                                         <  2:876803/140‑1 (MQ=255)                                                                                          gggATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCgtggtggt                                        <  1:213174/140‑1 (MQ=255)                                                                                              tatGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACg                                    <  2:59196/140‑1 (MQ=255)                                                                                                tggctggcGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGat                                  <  2:427969/140‑1 (MQ=255)                                                                                                tggctggcGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGat                                  <  2:569876/140‑1 (MQ=255)                                                                                                tggctggcGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGat                                  <  2:1201392/140‑1 (MQ=255)                                                                                                tggctggcGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGat                                  <  2:364966/140‑1 (MQ=255)                                                                                                    tggcGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGatcatc                              >  1:973440/1‑140 (MQ=255)                                                                                                    tggcGAGAAGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGatcatc                              >  1:1270822/1‑140 (MQ=255)                                                                                                           aaGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGATCATCTTTg                          >  1:314512/1‑137 (MQ=255)                                                                                                           aaGTCCGCGAGCGGCGAAAATTATTTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGATCATCTTTg                          <  2:314512/137‑1 (MQ=255)                                                                                                                                tattTGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGATCATCTTTGCTCTGCCGCCGATTATCCGTCTGa  <  2:68276/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        GCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCGTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTCGTCCACTGCTTGATGCCATGCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGACGATCATCTTTGCTCTGCCGCCGATTATCCGTCTGA  >  NC_000913/2806419‑2806685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑