Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,853,794	Δ1 bp	coding (541/1011 nt)	hypE →	hydrogenase maturation protein, carbamoyl dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,853,791	0	G	.	100.0%	152.9 / NA	37	coding (538/1011 nt)	hypE	hydrogenase maturation protein, carbamoyl dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/15);  total (22/15)

AGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTGATGCCACCCGTG  >  NC_000913/2853652‑2853926                                                                                                                                            |                                                                                                                                        aGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACg                                                                                                                                         <  2:467690/140‑1 (MQ=255) aGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACg                                                                                                                                         <  2:334488/140‑1 (MQ=255)     gtgCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcg                                                                                                                                     >  2:648534/1‑138 (MQ=255)      tgCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcga                                                                                                                                    >  1:820795/1‑137 (MQ=255)      tgCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATACACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcga                                                                                                                                    >  2:787828/1‑137 (MQ=255)       gCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcgac                                                                                                                                   <  1:474551/140‑5 (MQ=255)       gCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcgac                                                                                                                                   <  2:749026/140‑5 (MQ=255)       gCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcgac                                                                                                                                   <  1:243258/140‑5 (MQ=255)       gCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcgac                                                                                                                                   <  2:203312/140‑5 (MQ=255)          gcgcggcgcgGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTAt                                                                                                                               >  1:351450/1‑140 (MQ=255)            gcggcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATcc                                                                                                                             >  1:742130/1‑140 (MQ=255)             cggcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcgac                                                                                                                                   >  2:655121/1‑130 (MQ=255)             cggcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggcgac                                                                                                                                   <  1:655121/134‑5 (MQ=255)                cgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTaa                                                                                                                         >  1:777149/1‑140 (MQ=255)                        aTAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCCCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTg                                                                                                                 <  1:94094/140‑1 (MQ=255)                          aaaCTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGag                                                                                                               >  2:314512/1‑140 (MQ=255)                             cTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGagcag                                                                                                            <  2:777149/140‑1 (MQ=255)                               gTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCt                                                                                                          >  1:167591/1‑140 (MQ=255)                                 ttATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTgg                                                                                                        >  1:292527/1‑140 (MQ=255)                                     cAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCt                                                                                                    <  2:292527/140‑1 (MQ=255)                                          ccGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCACAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATg                                                                                               >  2:57619/1‑140 (MQ=255)                                              tGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATg                                                                                               >  2:528252/1‑136 (MQ=255)                                              tGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATg                                                                                               <  1:528252/136‑1 (MQ=255)                                                gCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAAc                                                                                         <  2:820795/140‑1 (MQ=255)                                                     ggCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTc                                                                                    >  1:252626/1‑140 (MQ=255)                                                     ggCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTc                                                                                    >  1:15333/1‑140 (MQ=255)                                                              ccGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTgc                                                                           <  2:167591/140‑1 (MQ=255)                                                               cGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTgcg                                                                          <  2:15333/140‑1 (MQ=255)                                                                         tCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGAc                                                                <  2:221636/140‑1 (MQ=255)                                                                         tCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGAc                                                                <  2:742130/140‑1 (MQ=255)                                                                               gggCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCt                                                          >  2:705545/1‑140 (MQ=255)                                                                                ggCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCtt                                                         >  1:261625/1‑140 (MQ=255)                                                                                  cgcACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTAt                                                       >  1:757673/1‑140 (MQ=255)                                                                                       aGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGa                                                  <  2:351450/140‑1 (MQ=255)                                                                                       aGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGa                                                  <  1:705545/140‑1 (MQ=255)                                                                                       aGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGa                                                  <  1:294562/140‑1 (MQ=255)                                                                                         aCGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACg                                                >  2:262729/1‑140 (MQ=255)                                                                                                 cGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGa                                        >  2:789806/1‑140 (MQ=255)                                                                                                 cGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGa                                        <  1:262729/140‑1 (MQ=255)                                                                                                   cAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACa                                      >  1:653355/1‑140 (MQ=255)                                                                                                     ggCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACAtt                                    <  1:787828/140‑1 (MQ=255)                                                                                                                    gTGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAgcg                     >  1:137077/1‑140 (MQ=255)                                                                                                                     tGAGCGGTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAgcgc                    >  2:800644/1‑140 (MQ=255)                                                                                                                           gTACACTCGGCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTg              >  1:563074/1‑140 (MQ=255)                                                                                                                                   ggCGACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTGATGCCAcc      >  1:484551/1‑140 (MQ=255)                                                                                                                                      gACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTGATGCCACCCgt   >  2:822840/1‑140 (MQ=255)                                                                                                                                      gACCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTGATGCCACCCgt   >  1:508665/1‑140 (MQ=255)                                                                                                                                       aCCAC‑GGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTGATGCCACCCgtg  <  2:484551/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        AGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGACGCCGCTTATTCAGACGCTGCGTGACATTCCCGGCGTGAAAGCGCTGCGTGATGCCACCCGTG  >  NC_000913/2853652‑2853926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑