Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,700	Δ1 bp	coding (108/213 nt)	yaiZ →	DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,697	0	A	.	100.0%	234.7 / NA	56	coding (105/213 nt)	yaiZ	DUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (28/28);  total (28/28)

GATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTA  >  NC_000913/399565‑399823                                                                                                                                     |                                                                                                                               gATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTg                                                                                                                        >  2:393087/1‑140 (MQ=255)   tgttgtTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGGCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGc                                                                                                                      <  2:61424/140‑1 (MQ=255)   tgttgtTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGc                                                                                                                      >  2:128234/1‑140 (MQ=255)   tgttgtTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGc                                                                                                                      >  2:717833/1‑140 (MQ=255)   tgttgtTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGc                                                                                                                      >  2:233991/1‑140 (MQ=255)        tttttCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGAcc                                                                                                                 <  1:124334/140‑1 (MQ=255)               ttAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCg                                                                                                          <  1:719417/140‑1 (MQ=255)                     gAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATACGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTc                                                                                                    >  1:664319/1‑140 (MQ=255)                            cATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATACGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGAc                                                                                             <  2:664319/140‑1 (MQ=255)                             aTGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACg                                                                                            <  2:703654/140‑1 (MQ=255)                                aaTCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGt                                                                                                         >  1:143779/1‑124 (MQ=255)                                aaTCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGt                                                                                                         <  2:143779/124‑1 (MQ=255)                                aaTCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTgg                                                                                         <  1:717833/140‑1 (MQ=255)                                     gCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGAt                                                                                    >  2:31019/1‑140 (MQ=255)                                     gCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGAt                                                                                    >  1:676228/1‑140 (MQ=255)                                      ccTGTAAAAATCCGCCGTGACTGGCACTACTATGTGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGAtt                                                                                   >  1:688052/1‑140 (MQ=255)                                      ccTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGAtt                                                                                   >  2:658970/1‑140 (MQ=255)                                      ccTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGAtt                                                                                   >  2:62295/1‑140 (MQ=255)                                                     cgTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCg                                                                    <  2:178150/140‑1 (MQ=255)                                                        gACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCgggg                                                                 <  1:658970/140‑1 (MQ=255)                                                                     aTGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATtcgtc                                                    >  1:40371/1‑140 (MQ=255)                                                                      tGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATtcgtcg                                                   <  2:24120/140‑1 (MQ=255)                                                                       gCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATtcgtcgt                                                  >  1:310320/1‑140 (MQ=255)                                                                        cGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATtcgtcgtc                                                 >  1:345740/1‑140 (MQ=255)                                                                        cGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGGTTATttgtcgtc                                                 >  2:620888/1‑140 (MQ=255)                                                                        cGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGATTATtcgtcgtc                                                 >  2:284961/1‑140 (MQ=255)                                                                           tCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTcgcg                                              >  1:371569/1‑140 (MQ=255)                                                                           tCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTcgcg                                              >  1:596302/1‑140 (MQ=255)                                                                             gCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTTGCGAt                                            >  1:558894/1‑140 (MQ=255)                                                                             gCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGAt                                            >  1:279466/1‑140 (MQ=255)                                                                             gCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGATTATTCGTCGTCGCGAt                                            >  2:197427/1‑140 (MQ=255)                                                                                aTTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATgaa                                         <  2:371569/140‑1 (MQ=255)                                                                                  tGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATgaaga                                       <  2:446484/140‑1 (MQ=255)                                                                                  tGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGATTATTCGTCGTCGCGATgaaga                                       >  1:703204/1‑140 (MQ=255)                                                                                           attcattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGaaaa                              <  1:393087/140‑1 (MQ=255)                                                                                              cattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGc                           <  2:40371/140‑1 (MQ=255)                                                                                              cattcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGc                           <  1:197427/140‑1 (MQ=255)                                                                                               attcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTTGCGATGAAGAAACTGAAAACGcc                          <  2:558894/140‑1 (MQ=255)                                                                                                ttcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGccc                         <  1:128234/140‑1 (MQ=255)                                                                                                 tcTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCa                        >  2:832517/1‑140 (MQ=255)                                                                                                   ttAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAt                      <  2:310320/140‑1 (MQ=255)                                                                                                   ttAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAt                      <  2:279466/140‑1 (MQ=255)                                                                                                   ttAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAt                      <  2:616257/140‑1 (MQ=255)                                                                                                   ttAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAt                      >  1:111341/1‑140 (MQ=255)                                                                                                    tAATGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGGTTATTCGGCGTCGCGATGAAGAAACTGAAAACGCCCAATa                     >  2:65787/1‑140 (MQ=255)                                                                                                      aTGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAg                   >  1:856573/1‑140 (MQ=255)                                                                                                       tGGCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGa                  <  1:65787/140‑1 (MQ=255)                                                                                                        ggCGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGaa                 <  1:62295/140‑1 (MQ=255)                                                                                                          cGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAt                      <  1:427179/133‑1 (MQ=255)                                                                                                          cGTGGTGGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGGTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAt                      >  2:427179/1‑133 (MQ=255)                                                                                                           gtggtgGGGTTACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAatat              >  1:648174/1‑140 (MQ=255)                                                                                                                     tACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGt    <  2:108770/140‑1 (MQ=255)                                                                                                                     tACTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGt    <  1:832517/140‑1 (MQ=255)                                                                                                                       cTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTa  <  1:620888/140‑1 (MQ=255)                                                                                                                       cTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTa  <  1:31019/140‑1 (MQ=255)                                                                                                                       cTGGGATTTGAAGC‑AAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTa  <  1:284961/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                               GATGTTGTTTTTCGTTAACGGAGCTGCCATGAATCTGCCTGTAAAAATCCGCCGTGACTGGCACTACTATGCGTTCGCCATTGGCCTTATATTCATTCTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTA  >  NC_000913/399565‑399823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑