Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	676,210	(G)5→4	coding (338/978 nt)	ybeQ ←	Sel1 repeat‑containing protein YbeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	676,206	0	G	.	100.0%	105.2 / NA	26	coding (342/978 nt)	ybeQ	Sel1 repeat‑containing protein YbeQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/12);  total (14/12)

GTACCAGAAAAATGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATTGGGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGT  >  NC_000913/676071‑676331                                                                                                                                        |                                                                                                                              gtACCAGAAAAATGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATTggggc                                                                                                                           <  1:422915/140‑2 (MQ=255) gtACCAGAAAAATGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATTggggc                                                                                                                           <  2:305607/140‑2 (MQ=255)   aCCAGAAAAATGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATTggggctt                                                                                                                         <  1:88252/140‑4 (MQ=255)       gAAAAATGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTcc                                                                                                                    >  2:344081/1‑140 (MQ=255)          aaaTGCCAGCGCATAGTCTTTTGCGACTTCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACg                                                                                                                 <  1:746995/140‑1 (MQ=255)          aaaTGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACg                                                                                                                 <  1:344081/140‑1 (MQ=255)             tGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATc                                                                                                              >  2:854254/1‑140 (MQ=255)                   cgcATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAgtgt                                                                                                        >  1:325371/1‑140 (MQ=255)                          tCTTTTGCGTCTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGACTTCTCCACGATCCAGTGTCCAGCCa                                                                                                 <  2:564537/140‑1 (MQ=255)                          tCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCa                                                                                                 <  2:96088/140‑1 (MQ=255)                               tGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGc                                                                                            >  1:841746/1‑140 (MQ=255)                                    cTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTgg                                                                                       >  1:241165/1‑140 (MQ=255)                                          tGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGa                                                                                 <  2:138910/140‑1 (MQ=255)                                                  cTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATgg                                                                         <  2:325371/140‑1 (MQ=255)                                                            ccccAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAggg                                                               <  1:854254/139‑1 (MQ=255)                                                               cAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAg                                                            >  1:146419/1‑140 (MQ=255)                                                                      attTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCtttttt                                                     <  1:474610/140‑1 (MQ=255)                                                                          tGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGc                                                 <  2:146419/140‑1 (MQ=255)                                                                            cgcATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCa                                               >  1:213603/1‑140 (MQ=255)                                                                                tAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCagaag                                           <  2:241165/140‑1 (MQ=255)                                                                                      aTTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCt                                     <  2:841746/140‑1 (MQ=255)                                                                                        tCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTg                                   >  1:847252/1‑140 (MQ=255)                                                                                        tCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTg                                   >  2:507182/1‑140 (MQ=255)                                                                                         ccGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGc                                  >  2:150386/1‑140 (MQ=255)                                                                                                              tACCAGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCtt             <  2:847252/140‑1 (MQ=255)                                                                                                                  aGACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTAt         <  1:150386/140‑1 (MQ=255)                                                                                                                   gACAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATc        >  2:307173/1‑140 (MQ=255)                                                                                                                    aCAACCGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATcc       >  2:2344/1‑140 (MQ=255)                                                                                                                        ccGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCgg   >  1:743190/1‑140 (MQ=255)                                                                                                                         cGCTTCTTTATAATT‑GGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGt  >  1:53406/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                              GTACCAGAAAAATGCCAGCGCATAGTCTTTTGCGACTCCGTTGCCATTTCTGTACATCCACCCAAGATTATTTTGCGCATAAGACATTCCGCTCTCCGCGGCTATCTGATACCAGACAACCGCTTCTTTATAATTGGGGGCTTCTCCACGATCCAGTGTCCAGCCAAGGGCGTTGGAAGCGAAAGTATGGCCTTGTAGGGCAGCTTTTTTCAGCCAGAAGATGGCTTGCTCATTATCTTTATGACGGCTTTTATCCTCGGT  >  NC_000913/676071‑676331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑