Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	915,225	(C)5→4	intergenic (‑368/+127)	lysO ← / ← aqpZ	L‑lysine exporter/water channel AqpZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,221	0	C	.	100.0%	133.1 / NA	36	intergenic (‑364/+131)	lysO/aqpZ	L‑lysine exporter/water channel AqpZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/21);  total (15/21)

GGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGC  >  NC_000913/915086‑915346                                                                                                                                        |                                                                                                                              gggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGcccct                                                                                                                           >  1:721971/1‑139 (MQ=17)   gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGcccctgc                                                                                                                         >  1:311444/1‑137 (MQ=17)      gAGGGCGCGTTTTACAGGGATGTTCCCTCGCGCCGGCCCGGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTgcgcg                                                                                                                     <  2:617632/140‑1 (MQ=11)      gAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTgcgcg                                                                                                                     <  1:313060/140‑1 (MQ=17)       aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTgcgcgc                                                                                                                    >  1:486229/1‑140 (MQ=17)                  tgcggggTTGTTCCCCCGCGCCCGCGCGGGTAGCCGTAAGGGATAAGTCGAGGGCCCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCa                                                                                                         <  2:633151/136‑1 (MQ=11)                  tACGGGGATTTTACCGCGCGCCGGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCa                                                                                                         <  1:110636/140‑1 (MQ=11)                       ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                    >  1:740567/1‑140 (MQ=18)                       ggATGTCACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGc                                                                                                    >  1:197212/1‑140 (MQ=255)                        gATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                   >  2:350578/1‑140 (MQ=21)                          tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAat                                                                                                 >  2:778363/1‑140 (MQ=37)                            ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAatat                                                                                               >  1:276997/1‑140 (MQ=255)                            ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAatat                                                                                               >  1:347278/1‑140 (MQ=255)                            ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAatat                                                                                               >  1:586855/1‑140 (MQ=255)                              aCCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                             >  2:592886/1‑140 (MQ=255)                                  cgcgCCCGACCGGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                         <  2:222475/140‑1 (MQ=255)                                  cgcgCCCGACCGGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                         <  1:135460/140‑1 (MQ=255)                                  cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                         <  2:197212/140‑1 (MQ=255)                                  cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                         <  2:740567/140‑1 (MQ=255)                                  cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                         <  2:586855/140‑1 (MQ=255)                                  cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                         <  2:347278/140‑1 (MQ=255)                                     gcCCGACCGGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                      <  1:140375/140‑1 (MQ=255)                                     gcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                      <  2:721971/140‑1 (MQ=255)                                     ccccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                      <  2:722804/139‑1 (MQ=255)                                                     gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAgcgc                                                                      >  2:707942/1‑140 (MQ=255)                                                             tAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgat                                                              >  2:304520/1‑140 (MQ=255)                                                                    aGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                       <  1:304520/140‑1 (MQ=255)                                                                         aCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACa                                                  >  2:744238/1‑140 (MQ=255)                                                                         aCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACa                                                  <  1:707942/140‑1 (MQ=255)                                                                                aGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaaca                                           <  2:821696/140‑1 (MQ=255)                                                                                                   agagCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                        <  1:350578/140‑1 (MQ=255)                                                                                                     agCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAgg                           <  1:582231/135‑1 (MQ=255)                                                                                                     agCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAgg                           >  2:582231/1‑135 (MQ=255)                                                                                                                gCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                        <  2:479054/127‑1 (MQ=255)                                                                                                                gCAGGGGGCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                        >  1:479054/1‑127 (MQ=255)                                                                                                                     gggCGGCGGCGATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCtt      <  2:276997/140‑1 (MQ=255)                                                                                                                           cggcgATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGc  <  1:432010/138‑1 (MQ=255)                                                                                                                           cggcgATTGGCCG‑CCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGc  >  2:432010/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                              GGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGC  >  NC_000913/915086‑915346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑