Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,987,851	(A)7→6	intergenic (‑93/+22)	yecJ ← / ← azuC	DUF2766 domain‑containing protein YecJ/uncharacterized protein AzuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,987,845	0	A	.	100.0%	157.2 / NA	38	intergenic (‑87/+28)	yecJ/azuC	DUF2766 domain‑containing protein YecJ/uncharacterized protein AzuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/19);  total (19/19)

CAAGCAACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTG  >  NC_000913/1987706‑1987979                                                                                                                                            |                                                                                                                                       caagcaaCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTGGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGAcaca                                                                                                                                        >  1:77026/1‑140 (MQ=255)      aaCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACaaaaaa                                                                                                                                   <  2:148853/140‑1 (MQ=255)                gAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAg                                                                                                                        <  1:852375/140‑1 (MQ=255)                  aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCa                                                                                                                      >  2:285238/1‑140 (MQ=255)                  aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCa                                                                                                                      >  1:660848/1‑140 (MQ=255)                   aCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAg                                                                                                                     >  1:443784/1‑140 (MQ=255)                      aaTTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGtt                                                                                                                  >  2:253401/1‑140 (MQ=255)                      aaTTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGtt                                                                                                                  >  2:595011/1‑140 (MQ=255)                              aTCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTa                                                                                                          >  2:171575/1‑140 (MQ=255)                               tCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTAt                                                                                                         <  1:171575/140‑1 (MQ=255)                                  gCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCgg                                                                                                      <  1:595011/140‑1 (MQ=255)                                   cATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGa                                                                                                     >  2:365216/1‑140 (MQ=255)                                   cATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGa                                                                                                     >  2:750025/1‑140 (MQ=255)                                   cATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGa                                                                                                     <  1:731697/140‑1 (MQ=255)                                                    aTCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGt                                                                                    >  2:839860/1‑140 (MQ=255)                                                     tCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTa                                                                                   <  2:268309/140‑1 (MQ=255)                                                     tCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTa                                                                                   <  2:159619/140‑1 (MQ=255)                                                      cGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                  >  1:821167/1‑140 (MQ=255)                                                                tGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGTGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                        >  1:744166/1‑140 (MQ=255)                                                                tGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                        >  1:329820/1‑140 (MQ=255)                                                                 gATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCTCATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACg                                                                       <  2:754176/140‑1 (MQ=255)                                                                   tAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                     <  1:750025/140‑1 (MQ=255)                                                                   tAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTc                                                                     <  1:285238/140‑1 (MQ=255)                                                                      cATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg                                                                  >  2:95141/1‑140 (MQ=255)                                                                       aTTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGc                                                                 <  2:688407/140‑1 (MQ=255)                                                                         tttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCaa                                                               >  2:480007/1‑140 (MQ=255)                                                                                           agCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACtt                                             >  2:123314/1‑140 (MQ=255)                                                                                              gACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTc                                          >  1:561258/1‑140 (MQ=255)                                                                                               aCTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACa                                                  >  1:137952/1‑131 (MQ=255)                                                                                               aCTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACa                                                  <  2:137952/131‑1 (MQ=255)                                                                                               aCTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCa                                         >  2:5883/1‑140 (MQ=255)                                                                                                         ttCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgcg                               <  2:117533/140‑1 (MQ=255)                                                                                                                                 catcaGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc       <  2:443784/140‑1 (MQ=255)                                                                                                                                 catcaGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc       <  2:561258/140‑1 (MQ=255)                                                                                                                                 catcaGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc       <  1:95141/140‑1 (MQ=255)                                                                                                                                 catcaGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc       <  1:480007/140‑1 (MQ=255)                                                                                                                                 catcaGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc       <  1:437533/140‑1 (MQ=255)                                                                                                                                 catcaGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAcc       <  1:331011/140‑1 (MQ=255)                                                                                                                                    caGGACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAtt    >  1:13171/1‑140 (MQ=255)                                                                                                                                      ggACAC‑AAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTg  <  1:839860/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       CAAGCAACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTG  >  NC_000913/1987706‑1987979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑