Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,037,280	(T)5→6	coding (827/1734 nt)	recJ ←	ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,037,275	1	.	T	100.0%	180.8 / NA	52	L278I (CTC→ATC)	recJ	ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (23/29);  total (23/29)

TCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAG‑TTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCA  >  NC_000913/3037139‑3037406                                                                                                                                          |                                                                                                                                    tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCACTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAG‑ttt                                                                                                                                  >  2:212258/1‑140 (MQ=255)    aGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAG‑tttttt                                                                                                                               <  1:301626/140‑2 (MQ=255)          tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCAt                                                                                                                          <  1:70120/140‑1 (MQ=255)                  cACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGttt                                                                                                                  >  2:120753/1‑140 (MQ=255)                    cgcgcgCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGc                                                                                                                <  1:120753/140‑1 (MQ=255)                     gcgcgcTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGcc                                                                                                               <  1:321202/140‑1 (MQ=255)                           ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGAAAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCa                                                                                                         >  2:773282/1‑140 (MQ=255)                             cGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAag                                                                                                       <  1:87940/140‑1 (MQ=255)                             cGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAag                                                                                                       <  2:372519/140‑1 (MQ=255)                              gCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCACTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAagc                                                                                                      <  1:212258/140‑1 (MQ=255)                               ccGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAagca                                                                                                     <  1:229434/140‑1 (MQ=255)                                     ttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                                               <  2:23788/140‑1 (MQ=255)                                               acaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCgg                                                                                     >  1:271958/1‑140 (MQ=255)                                                   caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACgg                                                                                 <  1:691487/140‑1 (MQ=255)                                                      cgcCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAc                                                                              >  2:643831/1‑140 (MQ=255)                                                      cgcCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAc                                                                              >  1:18254/1‑140 (MQ=255)                                                        ccACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACtt                                                                            >  1:779894/1‑140 (MQ=255)                                                        ccACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACtt                                                                            >  2:465787/1‑140 (MQ=255)                                                           cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGa                                                                                    <  2:355552/129‑1 (MQ=255)                                                           cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGa                                                                                    >  1:355552/1‑129 (MQ=255)                                                           cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTcc                                                                         >  1:147161/1‑140 (MQ=255)                                                               gACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCt                                                                     <  1:465787/140‑1 (MQ=255)                                                                    aCATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGAt                                                                <  1:315528/140‑1 (MQ=255)                                                                     cATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATg                                                               <  2:61597/140‑1 (MQ=255)                                                                     cATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATg                                                               <  2:271958/140‑1 (MQ=255)                                                                     cATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATg                                                               >  2:137935/1‑140 (MQ=255)                                                                          cGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                                                          >  2:795665/1‑140 (MQ=255)                                                                          cGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                                                          <  2:52802/140‑1 (MQ=255)                                                                          cGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                                                          >  2:369315/1‑140 (MQ=255)                                                                            tCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                                                          <  2:823641/138‑1 (MQ=255)                                                                            tCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACt                                                          >  1:823641/1‑138 (MQ=255)                                                                            tCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCa                                                        >  2:230159/1‑140 (MQ=255)                                                                              cAGTAGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATc                                                      >  2:867347/1‑140 (MQ=255)                                                                                gtcgtcCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATccc                                                    >  2:360282/1‑140 (MQ=255)                                                                                gtcgtcCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATccc                                                    >  1:269754/1‑140 (MQ=255)                                                                                   gtcCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTg                                                 <  1:23094/140‑1 (MQ=255)                                                                                         cagcaTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGt                                           <  2:18254/140‑1 (MQ=255)                                                                                         cagcaTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGt                                           <  2:779894/140‑1 (MQ=255)                                                                                             aTTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGa                                       <  1:643831/140‑1 (MQ=255)                                                                                                  gaCGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCg                                  <  1:334835/140‑1 (MQ=255)                                                                                                  gaCGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCg                                  <  1:89025/140‑1 (MQ=255)                                                                                                   aCGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGa                                 >  1:732621/1‑140 (MQ=255)                                                                                                      tGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGatta                              >  1:409009/1‑140 (MQ=255)                                                                                                         ccccAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGAttatta                           <  1:34095/140‑1 (MQ=255)                                                                                                              gcgcAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTc                      <  1:773282/140‑1 (MQ=255)                                                                                                               cgcAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGc                         <  2:366731/136‑1 (MQ=255)                                                                                                               cgcAAAACCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGc                         >  1:366731/1‑136 (MQ=255)                                                                                                                     aCCTAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGc               <  2:678277/140‑1 (MQ=255)                                                                                                                        tAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCacg            <  1:244526/140‑1 (MQ=255)                                                                                                                        tAAATCGCTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCacg            <  1:867347/140‑1 (MQ=255)                                                                                                                               cTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCg     >  2:57965/1‑140 (MQ=255)                                                                                                                               cTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCg     <  1:57965/140‑1 (MQ=255)                                                                                                                               cTGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCg     >  1:494384/1‑140 (MQ=255)                                                                                                                                tGGCGGCGAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGc    >  1:119011/1‑140 (MQ=255)                                                                                                                                  gcggcgAGTTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCa  >  1:588444/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    TCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAG‑TTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCA  >  NC_000913/3037139‑3037406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑