| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,184,476 | (A)7→8 | intergenic (‑10/‑364) | ribB ← / → ubiK | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,184,469 | 1 | . | A | 94.0% | 166.0 / 2.3 | 50 | intergenic (‑3/‑371) | ribB/ubiK | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (20/27); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑AAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATCCGGACTCTAACCGT > NC_000913/3184336‑3184600 | aTCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑aaaaaa > 2:526735/1‑140 (MQ=255) atatCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑aaaaaa‑aac < 2:658988/140‑3 (MQ=255) atCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑aaaaaa‑aacct < 2:726487/140‑5 (MQ=255) atCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑aaaaaa‑aacct > 2:202287/1‑136 (MQ=255) aCCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCAc < 2:358584/140‑1 (MQ=255) cTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑aaaaaa‑aac > 2:342892/1‑131 (MQ=255) cTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑aaaaaa‑aac < 1:342892/133‑3 (MQ=255) cGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTaaaa > 1:486626/1‑140 (MQ=255) cGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTaaaa > 1:77265/1‑140 (MQ=255) gTTTTCACGGTCTTCATCATCAAGCGCCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAAt < 1:104665/140‑1 (MQ=255) cACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATgg < 1:29713/140‑1 (MQ=255) cACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATgg < 1:311631/140‑1 (MQ=255) cACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATgg < 1:489371/140‑1 (MQ=255) tCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTAcc < 1:358518/140‑1 (MQ=255) ttCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTa > 2:179894/1‑130 (MQ=255) ttCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTa < 1:179894/130‑1 (MQ=255) ttCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAg > 1:243703/1‑140 (MQ=255) atcaAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAg > 1:809658/1‑140 (MQ=255) tcaAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAgg < 2:486626/140‑1 (MQ=255) tcaAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAgg < 2:496129/140‑1 (MQ=255) caAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAggg > 1:508024/1‑140 (MQ=255) aGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCa > 2:231666/1‑140 (MQ=255) gCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAg < 2:809658/140‑1 (MQ=255) aCCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTc > 2:559755/1‑140 (MQ=255) tACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAgg < 1:559755/140‑1 (MQ=255) tACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑AAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGa > 2:64517/1‑140 (MQ=255) tACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTACCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAgg < 2:761479/140‑1 (MQ=255) gcgTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggc < 2:565377/140‑1 (MQ=255) cgTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggcg > 2:856169/1‑140 (MQ=255) cgTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggcg > 1:739331/1‑140 (MQ=255) cgTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTggcg > 1:553900/1‑140 (MQ=255) ccTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCa < 2:479684/140‑1 (MQ=255) cTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCat < 1:214973/140‑1 (MQ=255) gcgcagcCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCATGGGA‑AAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGACATATAGCCaaa > 1:497012/1‑140 (MQ=255) gcgcagcCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCaaa > 2:15477/1‑140 (MQ=255) gcCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAAc < 1:412961/140‑1 (MQ=255) tGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATATCCAAAATAGCGTGAg < 2:77265/140‑1 (MQ=255) gCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGc > 1:707817/1‑140 (MQ=255) ttCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTc > 2:369890/1‑140 (MQ=255) acacGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATg > 1:631167/1‑140 (MQ=255) gAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAg < 1:856169/140‑1 (MQ=255) gAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAg < 1:483000/140‑1 (MQ=255) gTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGtt < 2:707817/140‑1 (MQ=255) gTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGtt < 2:243703/140‑1 (MQ=255) tACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTa > 2:383201/1‑140 (MQ=255) tACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTa > 2:395967/1‑140 (MQ=255) tACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTa > 1:195701/1‑140 (MQ=255) tACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTa > 1:340560/1‑140 (MQ=255) aCCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTAc < 2:508024/140‑1 (MQ=255) aaaaGAGGAAAGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTActct < 1:202287/140‑1 (MQ=255) aaaGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATc < 2:195701/140‑1 (MQ=255) aGTAGCGTCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATCCg < 1:395967/140‑1 (MQ=255) aGTAGCGTCTGATTCAT‑GGT‑AAAAAACACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGACATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGCCAGAATCGTTCCTCTCTCCCATCCg < 2:497012/140‑1 (MQ=255) tCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATc < 2:169771/131‑1 (MQ=255) tCTGATTCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATc > 1:169771/1‑131 (MQ=255) ttCAT‑GGTAAAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATCCGGACTCTAACCGt < 2:340560/140‑1 (MQ=255) | ATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTCGAAAGGCGTACCAAAAGAGGAAAGTAGCGTCTGATTCAT‑GGT‑AAAAAA‑ACCTCACTAAAATTATGGTTACCAGAATCAGGGCAGTCTTAGGAGTGGCGGCATATAGCCAAAATAACGTGAGCGGGTCCATGCCCGACAGAATCGTTACTCTCTCCCATCCGGACTCTAACCGT > NC_000913/3184336‑3184600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |