| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,474,275 | (C)6→7 | coding (152/594 nt) | bdcR → | putative transcriptional regulator BdcR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,474,269 | 1 | . | C | 94.7% | 125.7 / 0.2 | 38 | coding (146/594 nt) | bdcR | putative transcriptional regulator BdcR |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (18/18); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑A‑CCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGG > NC_000913/4474131‑4474403 | cTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑A‑c < 1:143367/140‑1 (MQ=255) gTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTACC‑CCCCCCGAGCCTCTAcg < 2:178117/140‑1 (MQ=255) gTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTAcg < 2:291195/140‑1 (MQ=255) cAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGc > 2:306647/1‑140 (MQ=255) tcgtcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTCGCTGAAGTTACTGATTATCTTGGTATTACC‑CCCCCCGAGCCTCTACGCGGCtttt < 2:134570/140‑1 (MQ=255) cgtcCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTg > 2:663947/1‑140 (MQ=255) cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCa > 2:400015/1‑140 (MQ=255) cccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCa > 1:817243/1‑140 (MQ=255) ccgaaCGTTTCGCTCCTGAGGAGGCAATCTCGGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGGCAGTGTTGGTAAAGTTAGTTATTATTTTGGTATTAGC‑CCCCCCGAGCCGCTACGCGGCTTTTGGCAg < 2:579652/136‑1 (MQ=255) ccAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAg < 2:705959/140‑1 (MQ=255) gTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAg > 2:53258/1‑139 (MQ=255) gTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAg < 1:53258/139‑1 (MQ=255) cGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTggg < 1:663947/140‑1 (MQ=255) tCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTa > 1:300726/1‑140 (MQ=255) cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt < 2:817243/140‑1 (MQ=255) cTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTAtt < 2:107903/140‑1 (MQ=255) cAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGcc < 2:300726/140‑1 (MQ=255) gCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCgtg < 1:306647/140‑1 (MQ=255) ctctGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTc > 1:53952/1‑140 (MQ=255) cGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGa > 2:285013/1‑140 (MQ=255) ggCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGaa < 1:514979/140‑1 (MQ=255) aaGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGt > 2:68863/1‑140 (MQ=255) ggTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCCTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGc > 2:116576/1‑140 (MQ=255) gTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCt < 1:68863/140‑1 (MQ=255) tCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGc < 2:53952/140‑1 (MQ=255) gATGCTGTCAGTGTGGCTGAAGTTCCTGATTATCTTGGTATTATC‑CCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCg < 2:463029/140‑1 (MQ=255) tGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATAtt < 2:559933/140‑1 (MQ=255) tGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATAtt > 2:153398/1‑140 (MQ=255) gCTGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGtgatg > 1:700079/1‑140 (MQ=255) tGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGatat < 2:173873/127‑1 (MQ=255) tGAAGTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGatat > 1:173873/1‑127 (MQ=255) gTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTc < 2:725775/140‑1 (MQ=255) gTTACTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTAGCGATGATCGTc > 1:762871/1‑140 (MQ=255) aCTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCgg < 1:583922/140‑1 (MQ=255) aCTGATTATCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCgg > 2:583922/1‑140 (MQ=255) aTCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGa > 2:609913/1‑140 (MQ=255) aTCTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGa < 1:285013/140‑1 (MQ=255) cTTGGTATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGt > 2:322164/1‑140 (MQ=255) tATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGc > 2:24482/1‑137 (MQ=255) tATTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGc < 1:24482/137‑1 (MQ=255) aTTA‑ACCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTgg < 1:549777/140‑1 (MQ=255) | CTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATTA‑A‑CCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGG > NC_000913/4474131‑4474403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |