Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	100.0%	103.9 / NA	32	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/15);  total (17/15)

TTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAT  >  NC_000913/4606183‑4606444                                                                                                                                             |                                                                                                                              tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                <  1:114496/140‑1 (MQ=255)        tCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggggg‑g                                                                                                                       <  2:611417/140‑1 (MQ=255)                     tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAAcccc                                                                                                         >  2:78588/1‑140 (MQ=255)                       ggAGGGGGGGGACTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCa                                                                                                       <  1:690571/139‑1 (MQ=255)                       cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCa                                                                                                       <  1:700712/140‑1 (MQ=255)                         aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACg                                                                                                     >  2:173267/1‑140 (MQ=255)                         aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCACCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACg                                                                                                     >  1:792790/1‑140 (MQ=255)                          ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGt                                                                                                    >  1:342000/1‑140 (MQ=255)                           gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCTATATCAACGTGGTTTTTCCTGCGA‑GGGGGGTTGT‑C‑TTGGATCCCCACGtt                                                                                                   >  2:847343/1‑139 (MQ=18)                                 gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAg                                                                                             <  1:600064/140‑1 (MQ=255)                                              aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGAGCGA‑GGGGGG‑GGATC‑TTCAACCCCCACGTCCGAAAGGACACTAACACCt                                                                                >  2:735587/1‑140 (MQ=37)                                                 tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAgg                                                                                            >  2:577375/1‑125 (MQ=255)                                                 tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAgg                                                                                            <  1:577375/125‑1 (MQ=255)                                                     tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                         >  1:854243/1‑140 (MQ=255)                                                          acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                    <  2:342000/140‑1 (MQ=255)                                                               aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTATGAGTAATTTCAACGTGGTTTTAGCAGCGA‑GGGGGG‑TGA‑CATAGAATCCCCACGTTCCAAATAACACTAACTTCTGAACCTAGcgcgcttac                                                               >  2:630913/1‑135 (MQ=12)                                                                        aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCgt                                                      <  1:310366/140‑2 (MQ=255)                                                                        aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                      >  2:579102/1‑140 (MQ=255)                                                                          cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                    <  1:173267/140‑1 (MQ=255)                                                                          cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                    <  1:525809/140‑1 (MQ=255)                                                                            aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACTTCCGGAAAGAAACTAACACCTGAAGCCATCGGGTCCTCCAATTCCGCCAcc                                                  >  2:678672/1‑140 (MQ=25)                                                                              cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                >  1:415997/1‑140 (MQ=255)                                                                              cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                >  1:731480/1‑140 (MQ=255)                                                                                  tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                            >  1:219173/1‑140 (MQ=255)                                                                                   cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                           <  2:792790/140‑1 (MQ=255)                                                                                           tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                   >  2:633305/1‑140 (MQ=255)                                                                                            ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt                                  <  1:633305/140‑1 (MQ=255)                                                                                                     tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa                         >  2:76282/1‑140 (MQ=255)                                                                                                         ccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                     <  1:548253/140‑1 (MQ=255)                                                                                                         ccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                     <  1:735587/140‑1 (MQ=255)                                                                                                             tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                 >  2:670668/1‑140 (MQ=255)                                                                                                                   cAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg           <  2:415997/140‑1 (MQ=255)                                                                                                                   cAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg           <  1:696977/140‑1 (MQ=255)                                                                                                                   cAATATCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg           <  2:219173/140‑1 (MQ=255)                                                                                                                       atCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg       >  2:407298/1‑140 (MQ=255)                                                                                                                       atCAACGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg       >  1:321603/1‑140 (MQ=255)                                                                                                                            cGTGGTTTTTGGTGCGAGGGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAt  >  2:348145/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                              TTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGG‑GGA‑C‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAT  >  NC_000913/4606183‑4606444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑