Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,612	T→C	S80G (AGC→GGC)	hycB ←	formate hydrogenlyase subunit HycB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,612	0	T	C	100.0%	92.0 / NA	27	S80G (AGC→GGC)	hycB	formate hydrogenlyase subunit HycB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/18);  total (9/18)

CCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCAT  >  NC_000913/2849480‑2849732                                                                                                                                     |                                                                                                                         ccGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCttcattc                                                                                                                   >  2:970/1‑140 (MQ=255)   ggAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCaa                                                                                                                 <  2:644462/140‑1 (MQ=255)        aGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCa                                                                                                            <  2:215414/140‑1 (MQ=255)                    tGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAGTTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCATACGCACAGGCCTTCATTCAACTGc                                                                                                             >  1:587825/1‑127 (MQ=255)                    tGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAGTTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCATACGCACAGGCCTTCATTCAACTGc                                                                                                             <  2:587825/127‑1 (MQ=255)                         cTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCgg                                                                                           <  1:656323/140‑1 (MQ=255)                            tCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCTGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGTACGGCCCCATCGACGCGGGTg                                                                                        <  2:418057/140‑1 (MQ=255)                             cGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGa                                                                                       <  1:970/140‑1 (MQ=255)                                    ttGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGtt                                                                                >  2:797813/1‑140 (MQ=255)                                            tGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc                                                                        >  2:2842/1‑140 (MQ=255)                                            tGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGc                                                                        >  2:625014/1‑140 (MQ=255)                                              ccGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAg                                                                      >  2:347466/1‑140 (MQ=255)                                                  aaTATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGacca                                                                  <  1:739423/140‑1 (MQ=255)                                                     atCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACcg                                                               <  2:559700/140‑1 (MQ=255)                                                       ccAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACcgcg                                                             <  1:760780/140‑1 (MQ=255)                                                          gCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACcgcg                                                             >  1:701249/1‑137 (MQ=255)                                                          gCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACcgcg                                                             <  2:701249/137‑1 (MQ=255)                                                                       ggAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTcac                                             <  1:347466/140‑1 (MQ=255)                                                                          aaaTTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAgt                                          <  2:606639/140‑1 (MQ=255)                                                                           aaTTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAgtg                                         <  1:2842/140‑1 (MQ=255)                                                                                                  ggCGATGCCGCACAGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGAtt                  <  1:81075/140‑1 (MQ=255)                                                                                                            cacaGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTttcatt        <  2:776840/140‑1 (MQ=255)                                                                                                             acaGCTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGTAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTttcattc       >  1:155973/1‑140 (MQ=255)                                                                                                                 cTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCa   <  2:682222/140‑1 (MQ=255)                                                                                                                 cTTGCAGCTTACGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCa   <  1:29729/140‑1 (MQ=255)                                                                                                                           aCGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTC‑TTTTCATTCAGCAt  <  1:737561/130‑1 (MQ=255)                                                                                                                           aCGCACAGGCCTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTC‑TTTTCATTCAGCAt  >  2:737561/1‑130 (MQ=255)                                                                                                                                     |                                                                                                                         CCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCCATCGACGCGGGTGATGGCGTTAACCGGGCAGACCACCGCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCAT  >  NC_000913/2849480‑2849732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑