Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,868,295	T→C	A153A (GCA→GCG)	nlpD ←	murein hydrolase activator NlpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,868,295	0	T	C	100.0%	231.5 / NA	66	A153A (GCA→GCG)	nlpD	murein hydrolase activator NlpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/35);  total (31/35)

TGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGA  >  NC_000913/2868172‑2868430                                                                                                                            |                                                                                                                                        tgCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTgc                                                                                                                         >  1:601128/1‑140 (MQ=255)  gCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTgcg                                                                                                                        <  2:601128/140‑1 (MQ=255)   cAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTgcgc                                                                                                                       >  2:513699/1‑140 (MQ=255)    aGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCt                                                                                                                      >  2:423570/1‑140 (MQ=255)           aTCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAg                                                                                                               >  2:98464/1‑140 (MQ=255)             cacaACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGt                                                                                                             <  1:512505/140‑1 (MQ=255)                aaCTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCAc                                                                                                          >  1:867684/1‑140 (MQ=255)                aaCTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCAc                                                                                                          >  1:756455/1‑140 (MQ=255)                     cTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGaaa                                                                                                     >  1:763887/1‑140 (MQ=255)                       tGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATc                                                                                                   >  1:353197/1‑140 (MQ=255)                          tctGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGtt                                                                                                >  2:784386/1‑140 (MQ=255)                             gctgcGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGcc                                                                                             >  1:38148/1‑140 (MQ=255)                             gctgcGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGcc                                                                                             >  1:491092/1‑140 (MQ=255)                              ctgcGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGtt                                                                                                >  2:782819/1‑136 (MQ=255)                              ctgcGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGtt                                                                                                <  1:782819/136‑1 (MQ=255)                              ctgcGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCa                                                                                            >  1:777786/1‑140 (MQ=255)                               tgcGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAg                                                                                           <  2:756455/140‑1 (MQ=255)                                        cTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAgg                                                                                  <  1:840035/140‑1 (MQ=255)                                        cTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAgg                                                                                  <  1:513699/140‑1 (MQ=255)                                          gggTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCg                                                                                <  1:107343/140‑1 (MQ=255)                                          gggTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCg                                                                                <  1:422041/140‑1 (MQ=255)                                           ggTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGa                                                                               >  1:741968/1‑140 (MQ=255)                                                tGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAgg                                                                                  <  1:651992/132‑1 (MQ=255)                                                tGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAgg                                                                                  <  1:10347/132‑1 (MQ=255)                                                tGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAgg                                                                                  >  2:651992/1‑132 (MQ=255)                                                tGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAgg                                                                                  >  2:10347/1‑132 (MQ=255)                                                tGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAg                                                                          >  1:672630/1‑140 (MQ=255)                                                tGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAg                                                                          >  2:12430/1‑140 (MQ=255)                                                       tCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGata                                                                             >  1:801473/1‑130 (MQ=255)                                                       tCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGata                                                                             <  2:801473/130‑1 (MQ=255)                                                                 aTTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTAAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCttt                                                         >  2:736979/1‑140 (MQ=255)                                                                     gCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTc                                                     <  2:777786/140‑1 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTc                                                     >  2:85843/1‑139 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTc                                                     <  1:85843/139‑1 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    <  2:867684/140‑1 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    >  1:281250/1‑140 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    <  1:495790/140‑1 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    <  2:491092/140‑1 (MQ=255)                                                                      cGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    <  1:98464/140‑1 (MQ=255)                                                                       gTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCAc                                                   <  2:271583/140‑1 (MQ=255)                                                                       gTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCAc                                                   <  1:12430/140‑1 (MQ=255)                                                                       gTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCAc                                                   <  2:287626/140‑1 (MQ=255)                                                                       gTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCAc                                                   <  2:129051/140‑1 (MQ=255)                                                                       gTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCAc                                                   <  1:423570/140‑1 (MQ=255)                                                                           cGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGta                                               <  1:94570/140‑1 (MQ=255)                                                                                   ttACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACt                                       <  2:281250/140‑1 (MQ=255)                                                                                     aCCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    <  2:618402/125‑1 (MQ=255)                                                                                     aCCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCa                                                    >  1:618402/1‑125 (MQ=255)                                                                                        cACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGc                                  <  1:217944/140‑1 (MQ=255)                                                                                              cAAGGTCTGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAAc                            >  1:864318/1‑140 (MQ=255)                                                                                                     tGACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCttt                     >  2:333870/1‑140 (MQ=255)                                                                                                      gACCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTc                    >  1:396787/1‑140 (MQ=255)                                                                                                       aCCAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCg                   >  2:571540/1‑140 (MQ=255)                                                                                                        ccAACGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCgg                  >  2:51054/1‑140 (MQ=255)                                                                                                          aaCGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGaa                <  2:38148/140‑1 (MQ=255)                                                                                                          aaCGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGaa                <  2:867282/140‑1 (MQ=255)                                                                                                          aaCGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGaa                <  1:333870/140‑1 (MQ=255)                                                                                                          aaCGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGaa                <  1:554615/140‑1 (MQ=255)                                                                                                          aaCGTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGaa                <  1:571540/140‑1 (MQ=255)                                                                                                          aaCGTTAAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGaa                <  1:736979/140‑1 (MQ=255)                                                                                                             gTTCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGt             >  1:673954/1‑140 (MQ=255)                                                                                                              ttCAGCGCGTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGtt            <  2:353197/140‑1 (MQ=255)                                                                                                                      gTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTGATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACt    >  1:527089/1‑140 (MQ=255)                                                                                                                      gTATGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACt    >  1:669155/1‑140 (MQ=255)                                                                                                                        aTGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGa  <  2:669155/140‑1 (MQ=255)                                                                                                                        aTGGCGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGa  <  1:51054/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                        TGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCACCTGCAAGGTCTGACCAACGTTCAGCGCGTATGGTGCCTGAATATTGTTGCGCTGAGCAAGGTCACGGAAATCGTTGCCAGTAATCCAGGCGATATAGAAAAGTGTGTCGCCTTTTTTCACGGTATAGGTACTGCCGCTATAACTGCCTTTCGGAATGTTCCCATACTGA  >  NC_000913/2868172‑2868430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑