Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,937,348	T→C	Y589H (TAC→CAC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,937,348	0	T	C	100.0%	187.2 / NA	53	Y589H (TAC→CAC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

TCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGA  >  NC_000913/2937211‑2937478                                                                                                                                          |                                                                                                                                   tcGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCAc                                                                                                                                  >  1:77785/1‑140 (MQ=255)  cGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGcaca                                                                                                                                 <  2:220678/140‑1 (MQ=255)  cGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGcaca                                                                                                                                 <  1:44803/140‑1 (MQ=255)   gCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACaa                                                                                                                                >  2:231845/1‑140 (MQ=255)       ccATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGt                                                                                                                            <  2:77785/140‑1 (MQ=255)       ccATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGt                                                                                                                            <  2:167970/140‑1 (MQ=255)       ccATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGt                                                                                                                            <  2:347825/140‑1 (MQ=255)       ccATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGt                                                                                                                            <  2:542687/140‑1 (MQ=255)         aTGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTa                                                                                                                          >  1:616998/1‑140 (MQ=255)          tgctgcGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTaa                                                                                                                         <  1:231845/140‑1 (MQ=255)          tgctgcGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTaa                                                                                                                         >  2:198960/1‑140 (MQ=255)                    cccGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGt                                                                                                                            >  1:752858/1‑127 (MQ=255)                    cccGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTCGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGt                                                                                                                            <  2:752858/127‑1 (MQ=255)                      cGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTccc                                                                                                             <  2:604961/140‑1 (MQ=255)                         tatgtatgCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCtga                                                                                                          >  1:440720/1‑140 (MQ=255)                                     aCGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCgg                                                                                              <  2:63742/140‑1 (MQ=255)                                     aCGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCgg                                                                                              >  1:63742/1‑140 (MQ=255)                                      cGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGt                                                                                             >  1:295769/1‑140 (MQ=255)                                      cGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGt                                                                                             <  2:616998/140‑1 (MQ=255)                                              agaCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCg                                                                                      <  1:393693/139‑1 (MQ=255)                                              agaCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCg                                                                                      >  2:393693/1‑139 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCtgatgtga                                                                                                     >  1:184298/1‑122 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCtgatgtga                                                                                                     <  2:184298/122‑1 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCg                                                                                      <  2:258009/137‑1 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCg                                                                                      >  1:258009/1‑137 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCt                                                                                   >  2:612602/1‑140 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCt                                                                                   >  2:146950/1‑140 (MQ=255)                                                aCCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCt                                                                                   >  1:368284/1‑140 (MQ=255)                                                  cAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTAc                                                                                 <  2:295769/140‑1 (MQ=255)                                                            cGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTGCGGCTACCGGGCCTGaa                                                                       <  1:639322/140‑1 (MQ=255)                                                                 ttCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAAcaagc                                                                  >  2:21364/1‑140 (MQ=255)                                                                   cTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAAcaagcaa                                                                >  2:391570/1‑140 (MQ=255)                                                                      ccTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAAcaag                                                                   >  1:425513/1‑134 (MQ=255)                                                                      ccTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAAcaag                                                                   <  2:425513/134‑1 (MQ=255)                                                                               cgcACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGcc                                                    >  1:170213/1‑140 (MQ=255)                                                                               cgcACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGcc                                                    >  2:642256/1‑140 (MQ=255)                                                                                 cACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCgg                                                  <  1:263863/140‑1 (MQ=255)                                                                                  aCGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATGCAATACTCCCTGATGTGATGCCCGGTCGCACCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGa                                                 <  1:107384/140‑1 (MQ=255)                                                                                  aCGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGa                                                 <  1:634798/140‑1 (MQ=255)                                                                                   cGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGAt                                                <  1:391570/140‑1 (MQ=255)                                                                                     gCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATaa                                              <  1:146950/140‑1 (MQ=255)                                                                                             atTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGtt                                      <  1:642256/140‑1 (MQ=255)                                                                                                gAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATc                                   >  1:703013/1‑140 (MQ=255)                                                                                                gAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATc                                   <  1:198960/140‑1 (MQ=255)                                                                                                   ggCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCgg                                >  1:19323/1‑140 (MQ=255)                                                                                                   ggCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCgg                                <  2:708483/140‑1 (MQ=255)                                                                                                    gCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGc                               >  2:611908/1‑140 (MQ=255)                                                                                                      cAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCta                             >  2:519394/1‑140 (MQ=255)                                                                                                      cAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCta                             >  2:724205/1‑140 (MQ=255)                                                                                                      cAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCta                             <  1:611908/140‑1 (MQ=255)                                                                                                        ggATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGc                               >  2:543603/1‑136 (MQ=255)                                                                                                        ggATTACCGCGCTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGc                               <  1:543603/136‑1 (MQ=255)                                                                                                                   cTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGc                       >  2:618677/1‑133 (MQ=255)                                                                                                                   cTTGCCAGAACTACGGTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGc                       <  1:618677/133‑1 (MQ=255)                                                                                                                                 ggTCCGTTGCACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACaaga  <  2:301207/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCTGAACAAGCAAGAGTGGTTAGCCGGATAAGCAATGTTATCCGGCTATATTGCAGGAGCGATTATGAAACAAGA  >  NC_000913/2937211‑2937478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑