Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	282,445	C→T	L56L (CTC→CTT)	yagE →	CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	282,445	0	C	T	100.0%	127.8 / NA	37	L56L (CTC→CTT)	yagE	CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/22);  total (15/22)

TCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGG  >  NC_000913/282310‑282575                                                                                                                                        |                                                                                                                                   ccccccTGTCTCAGCCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGgcg                                                                                                                                <  2:342479/139‑1 (MQ=255)      cTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGaa                                                                                                                           <  2:308972/140‑1 (MQ=255)        gTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAaga                                                                                                                         >  1:842448/1‑140 (MQ=255)            ccacca‑TTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTa                                                                                                                    <  1:526525/140‑1 (MQ=255)             caccaTTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTa                                                                                                                    <  1:197217/140‑1 (MQ=255)             caccaTTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTa                                                                                                                    <  1:154475/140‑1 (MQ=255)                 aTTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGc                                                                                                                >  2:730437/1‑140 (MQ=255)                       aCCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGc                                                                                                          >  1:7285/1‑140 (MQ=255)                        ccgccgACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGcc                                                                                                         <  2:87604/140‑1 (MQ=255)                        ccgccgACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGcc                                                                                                         <  2:421846/140‑1 (MQ=255)                        ccgccgACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGcc                                                                                                         <  2:720577/140‑1 (MQ=255)                            cgACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCt                                                                                                     <  2:589145/140‑1 (MQ=255)                            cgACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCt                                                                                                     <  2:636873/140‑1 (MQ=255)                             gACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCtt                                                                                                    <  1:730437/140‑1 (MQ=255)                                ggCCAGCTCGATAAGCCGGGCACCGCCGCGCTGGTCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGc                                                                                                 <  1:8369/140‑1 (MQ=255)                                 gCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCt                                                                                                <  2:36993/140‑1 (MQ=255)                                     gCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCg                                                                                            <  2:214668/140‑1 (MQ=255)                                                       cgccgcGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAt                                                                                 >  2:253337/1‑133 (MQ=255)                                                       cgccgcGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAt                                                                                 <  1:253337/133‑1 (MQ=255)                                                          cgcgCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGc                                                                       <  1:645343/140‑1 (MQ=255)                                                          cgcgCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGc                                                                       <  2:104527/140‑1 (MQ=255)                                                            cgcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCg                                                                     >  1:412721/1‑140 (MQ=255)                                                             gcTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCgg                                                                    <  1:78357/140‑1 (MQ=255)                                                                           tGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAcc                                                      >  2:490601/1‑140 (MQ=255)                                                                           tGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAcc                                                      >  2:184146/1‑140 (MQ=255)                                                                           tGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAcc                                                      >  2:333610/1‑140 (MQ=255)                                                                                   gCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCAc                                              >  2:170041/1‑140 (MQ=255)                                                                                       gCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAAc                                          >  1:81997/1‑140 (MQ=255)                                                                                               ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGa                                  <  2:2025/140‑1 (MQ=255)                                                                                               ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGa                                  <  2:733796/140‑1 (MQ=255)                                                                                                     ttcttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCAt                            >  2:403208/1‑140 (MQ=255)                                                                                                     ttcttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCAt                            <  1:170041/140‑1 (MQ=255)                                                                                                         tcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGaa                        >  1:496708/1‑140 (MQ=255)                                                                                                             gggCAGCGGTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTca                    >  2:226332/1‑140 (MQ=255)                                                                                                                     gTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCAc            >  1:61557/1‑140 (MQ=255)                                                                                                                     gTGGCGAGTTCTCCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCAc            >  1:297035/1‑140 (MQ=255)                                                                                                                               ctcCCAGCTTGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAgg  <  2:61557/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TCCCCCTGTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATCGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGG  >  NC_000913/282310‑282575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑