Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,763	T→C	V247A (GTT→GCT)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,763	0	T	C	100.0%	63.0 / NA	19	V247A (GTT→GCT)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/4);  total (15/4)

CGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACG  >  NC_000913/3208629‑3208886                                                                                                                                       |                                                                                                                            cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTctgc                                                                                                                        <  1:595503/140‑1 (MQ=255)   tGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTctgc                                                                                                                        <  2:694257/138‑1 (MQ=255)   tGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTctgc                                                                                                                        >  1:694257/1‑138 (MQ=255)             cATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCctggc                                                                                                            <  1:688241/140‑1 (MQ=255)                    ttGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTa                                                                                                     <  1:488042/140‑1 (MQ=255)                                gCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTAccc                                                                                         >  2:771526/1‑140 (MQ=255)                                                         tgatgaAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAg                                                                >  1:14333/1‑140 (MQ=255)                                                           atgaAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGt                                                              >  2:102218/1‑140 (MQ=255)                                                                   cgcATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCt                                                      >  1:336887/1‑140 (MQ=255)                                                                     cATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTgg                                                    >  1:310666/1‑140 (MQ=255)                                                                     cATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTgg                                                    >  2:245657/1‑140 (MQ=255)                                                                                  gcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAAc                                       >  1:713907/1‑140 (MQ=255)                                                                                  gcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAAc                                       >  1:842227/1‑140 (MQ=255)                                                                                  gcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAAc                                       >  1:845303/1‑140 (MQ=255)                                                                                  gcTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAAc                                       >  2:583598/1‑140 (MQ=255)                                                                                                aCTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc                         >  1:685156/1‑140 (MQ=255)                                                                                                aCTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc                         >  1:291069/1‑140 (MQ=255)                                                                                                               tGTTGCCGCTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGt          >  1:618430/1‑140 (MQ=255)                                                                                                                       cTCAGCATTTTACTGGCTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACg  >  2:636444/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                            CGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACG  >  NC_000913/3208629‑3208886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑