Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,210,770	G→A	intergenic (‑227/‑11)	tsaD ← / → rpsU	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit/30S ribosomal subunit protein S21

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,210,770	0	G	A	100.0%	180.2 / NA	52	intergenic (‑227/‑11)	tsaD/rpsU	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit/30S ribosomal subunit protein S21
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/25);  total (27/25)

TCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGA  >  NC_000913/3210638‑3210904                                                                                                                                     |                                                                                                                                       tcctcACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAgg                                                                                                                                 >  1:481655/1‑140 (MQ=255)   ctcACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGca                                                                                                                               >  1:696864/1‑140 (MQ=255)    tcACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGcac                                                                                                                              >  2:647948/1‑140 (MQ=255)      aCCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACAt                                                                                                                            >  2:486863/1‑140 (MQ=255)       cccTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATg                                                                                                                           <  2:696864/140‑1 (MQ=255)         cTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGcc                                                                                                                         <  2:722264/140‑1 (MQ=255)         cTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGcc                                                                                                                         <  1:530828/140‑1 (MQ=255)          ttATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCg                                                                                                                        <  1:647948/140‑1 (MQ=255)           tataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCgg                                                                                                                       <  1:340625/140‑1 (MQ=255)           tataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCgg                                                                                                                       >  1:474368/1‑140 (MQ=255)            ataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGt                                                                                                                      <  2:333749/140‑1 (MQ=255)             taAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGcc                                                                                                                         <  1:785820/136‑1 (MQ=255)             taAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGcc                                                                                                                         >  2:785820/1‑136 (MQ=255)                 aGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTa                                                                                                                 <  2:85848/140‑1 (MQ=255)                  gTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTaa                                                                                                                >  1:52516/1‑140 (MQ=255)                     ccTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGcc                                                                                                                         >  1:89335/1‑128 (MQ=255)                     ccTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGcc                                                                                                                         <  2:89335/128‑1 (MQ=255)                     ccTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGt                                                                                                             >  2:8847/1‑140 (MQ=255)                      cTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTa                                                                                                            >  1:693795/1‑140 (MQ=255)                      cTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTa                                                                                                            >  1:453624/1‑140 (MQ=255)                       tttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTAc                                                                                                           >  2:699968/1‑140 (MQ=255)                        ttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCg                                                                                                                        >  2:320142/1‑126 (MQ=255)                        ttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCg                                                                                                                        <  1:320142/126‑1 (MQ=255)                                aGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACg                                                                                                  <  1:8847/140‑1 (MQ=255)                                  gCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAg                                                                                                <  2:52516/140‑1 (MQ=255)                                       ggTGCTTTACAAAGCGGCAGCAATTGCAGTAAAATTCCGAACCATTTTGAAATAACCAGGCGTTGTGGCTGGCGGCAAACCGAATTAACCAAAGCTGGGAGGCACATGCCGGTAATTAAAGTACGTGAAAGCGAGCCGtt                                                                                           <  2:357932/140‑1 (MQ=255)                                       ggTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGtt                                                                                           <  2:24689/140‑1 (MQ=255)                                       ggTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGtt                                                                                           <  1:122301/140‑1 (MQ=255)                                                   agcagcagcAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGtt                                                                                           <  1:737321/128‑1 (MQ=255)                                                   agcagcagcAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGtt                                                                                           >  2:737321/1‑128 (MQ=255)                                                   agcagcagcAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGctct                                                                               >  2:295893/1‑140 (MQ=255)                                                     cagcagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGc                                                                             >  1:712326/1‑140 (MQ=255)                                                      agcagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAg                                                                                   >  2:827214/1‑133 (MQ=255)                                                      agcagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAg                                                                                   <  1:827214/133‑1 (MQ=255)                                                       gcagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGt                                                                           <  2:474368/140‑1 (MQ=255)                                                        cagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTc                                                                          >  2:666511/1‑140 (MQ=255)                                                          gcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGc                                                                        >  1:814107/1‑140 (MQ=255)                                                                                   ttttGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAg                                               >  1:590721/1‑140 (MQ=255)                                                                                           tAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTg                                       >  2:290645/1‑140 (MQ=255)                                                                                             aGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGc                                     >  1:811487/1‑140 (MQ=255)                                                                                             aGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGc                                     >  2:150776/1‑140 (MQ=255)                                                                                                  gCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTACGAAAAAGCAGGTGTTCTGGCGGAAg                                <  1:295893/140‑1 (MQ=255)                                                                                                   ggTTGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGt                               <  2:712326/139‑1 (MQ=255)                                                                                                      tGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcg                            <  2:811487/140‑1 (MQ=255)                                                                                                      tGATGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcg                            <  2:814107/140‑1 (MQ=255)                                                                                                        aTGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtc                          >  2:639554/1‑140 (MQ=255)                                                                                                        aTGCCAGCGGCAAACCGAATTAATCAAAGATGAGAGGAACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGAtcgtc                          <  2:481655/140‑1 (MQ=255)                                                                                                                    aaCCGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt              >  2:500855/1‑140 (MQ=255)                                                                                                                      ccGAATTAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTAt            <  1:666511/140‑1 (MQ=255)                                                                                                                         aaTAAATCAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGaa         <  1:35468/140‑1 (MQ=255)                                                                                                                                cAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGa  >  2:585418/1‑140 (MQ=255)                                                                                                                                cAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGa  >  2:801529/1‑140 (MQ=255)                                                                                                                                cAAAGATGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGa  <  2:453624/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGA  >  NC_000913/3210638‑3210904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑