Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,976	G→A	P251P (CCG→CCA)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,976	0	G	A	100.0%	137.7 / NA	40	P251P (CCG→CCA)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

AGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCGCTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTG  >  NC_000913/3245840‑3246109                                                                                                                                         |                                                                                                                                      aGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTg                                                                                                                                    >  2:832200/1‑140 (MQ=255) aGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTg                                                                                                                                    >  2:436631/1‑140 (MQ=255)    aaGCCCAGCACCAGGTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTc                                                                                                                                 <  2:689406/140‑1 (MQ=255)        ccAGCACCAGGTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGt                                                                                                                             >  2:434132/1‑140 (MQ=255)                 ggTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGttt                                                                                                                    >  1:352231/1‑140 (MQ=255)                 ggTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGttt                                                                                                                    >  2:254766/1‑140 (MQ=255)                  gTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTa                                                                                                                   <  1:254766/140‑1 (MQ=255)                     aGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACgg                                                                                                                >  1:642016/1‑140 (MQ=255)                       cACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTCTAAAGTTTACGGCt                                                                                                              >  1:234284/1‑140 (MQ=255)                        aCGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCtt                                                                                                             <  2:352231/140‑1 (MQ=255)                        aCGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCtt                                                                                                             <  2:314936/140‑1 (MQ=255)                          ggCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTa                                                                                                           <  1:83915/140‑1 (MQ=255)                          ggCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTa                                                                                                           <  1:51440/140‑1 (MQ=255)                            cGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAAc                                                                                                         <  2:588753/140‑1 (MQ=255)                            cGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAAc                                                                                                         >  1:187024/1‑140 (MQ=255)                                                cAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGtt                                                                                     >  2:452885/1‑140 (MQ=255)                                                        gCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGa                                                                             >  1:725826/1‑140 (MQ=255)                                                           tAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGc                                                                          <  2:680137/140‑1 (MQ=255)                                                             aCCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCg                                                                        >  2:123008/1‑140 (MQ=255)                                                                   aGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTg                                                                  >  1:370429/1‑140 (MQ=255)                                                                       cTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTg                                                              <  1:434132/140‑1 (MQ=255)                                                                              aTCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCt                                                       <  1:815006/140‑1 (MQ=255)                                                                               tCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCt                                                            >  1:663070/1‑134 (MQ=255)                                                                               tCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCt                                                            <  2:663070/134‑1 (MQ=255)                                                                               tCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTc                                                      >  2:793616/1‑140 (MQ=255)                                                                                   gcTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGtt                                                  <  2:359630/140‑1 (MQ=255)                                                                                          cgTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCt                                           <  1:123008/140‑1 (MQ=255)                                                                                          cgTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCt                                           <  1:793616/140‑1 (MQ=255)                                                                                                 tGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCggtggt                                    >  1:266660/1‑140 (MQ=255)                                                                                                    cAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTAc                                 >  1:248448/1‑140 (MQ=255)                                                                                                       aaCCGGCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTg                              >  2:606940/1‑140 (MQ=255)                                                                                                           ggCCTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTgccgc                          <  2:370429/140‑1 (MQ=255)                                                                                                             ccTGGGGTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGccgccg                        <  1:606940/140‑1 (MQ=255)                                                                                                                  ggTACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGtt                   <  1:670414/140‑1 (MQ=255)                                                                                                                    tACTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTcc                 <  1:452885/140‑1 (MQ=255)                                                                                                                      cTTTTAACGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAg               >  2:796497/1‑140 (MQ=255)                                                                                                                           aaCGCGTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGttg          >  2:598060/1‑140 (MQ=255)                                                                                                                              gcgTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGttggtt       <  1:377331/140‑1 (MQ=255)                                                                                                                              gcgTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGttggtt       <  1:406844/140‑1 (MQ=255)                                                                                                                                gTGGATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGttggtttg     <  2:187024/140‑1 (MQ=255)                                                                                                                                   gATCCCACTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGgtg  >  2:317990/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AGGAAGCCCAGCACCAGGTGAGCACGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAACCGGCCTGGGGTACTTTTAACGCGTGGATCCCGCTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGATGTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTG  >  NC_000913/3245840‑3246109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑