Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,247,296	G→A	W215* (TGG→TGA)	exuR →	DNA‑binding transcriptional repressor ExuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,247,296	0	G	A	100.0%	146.3 / NA	42	W215* (TGG→TGA)	exuR	DNA‑binding transcriptional repressor ExuR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/17);  total (25/17)

AACCCGTACTGGAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGA  >  NC_000913/3247168‑3247428                                                                                                                                 |                                                                                                                                     aaCCCGTACTGGAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGa                                                                                                                           >  2:64520/1‑140 (MQ=255)  aCCCGTACTGGAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGaa                                                                                                                          <  1:64520/140‑1 (MQ=255)       tACTGGAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAacac                                                                                                                     >  1:795996/1‑140 (MQ=255)       tACTGGAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAacac                                                                                                                     >  1:451407/1‑140 (MQ=255)                                     tCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGcga                                                                                       >  2:413121/1‑140 (MQ=255)                                     tCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGcga                                                                                       >  1:450705/1‑140 (MQ=255)                                            ccGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTc                                                                                <  2:450705/140‑1 (MQ=255)                                            ccGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTc                                                                                <  1:343337/140‑1 (MQ=255)                                               tCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAg                                                                             >  1:842143/1‑140 (MQ=255)                                               tCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAg                                                                             >  2:585136/1‑140 (MQ=255)                                                    aaCTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACt                                                                                  >  2:305260/1‑130 (MQ=255)                                                    aaCTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACt                                                                                  <  1:305260/130‑1 (MQ=255)                                                         gtgtGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCg                                                                   <  2:810301/140‑1 (MQ=255)                                                         gtgtGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCg                                                                   >  2:161775/1‑140 (MQ=255)                                                          tgtgATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGa                                                                  <  1:413121/140‑1 (MQ=255)                                                          tgtgATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGa                                                                  <  1:131154/140‑1 (MQ=255)                                                                 aCCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGctat                                                           >  1:770662/1‑140 (MQ=255)                                                                   cACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGctatct                                                         >  1:468241/1‑140 (MQ=255)                                                                    aCGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGctatcta                                                        >  1:304365/1‑140 (MQ=255)                                                                      gATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGcc                                                                    >  2:234417/1‑126 (MQ=255)                                                                      gATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGcc                                                                    <  1:234417/126‑1 (MQ=255)                                                                            aTCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGa                                                <  2:412735/140‑1 (MQ=255)                                                                                tCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAAc                                            >  1:647110/1‑140 (MQ=255)                                                                                  aaGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAAccc                                          >  2:860228/1‑140 (MQ=255)                                                                                      cgcTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCgg                                        <  1:643742/138‑1 (MQ=255)                                                                                      cgcTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCgg                                        >  2:643742/1‑138 (MQ=255)                                                                                       gcTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCggtgg                                     >  2:390713/1‑140 (MQ=255)                                                                                         tGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGgtggtg                                   <  1:860228/140‑1 (MQ=255)                                                                                         tGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGgtggtg                                   <  2:451407/140‑1 (MQ=255)                                                                                            ttCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCAt                                >  1:759848/1‑140 (MQ=255)                                                                                                cAAAGATCCTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCg                            <  2:468241/140‑1 (MQ=255)                                                                                                        cTCATGCTGCTAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGcc                    <  2:770662/140‑1 (MQ=255)                                                                                                               tgctAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCg             >  2:264001/1‑140 (MQ=255)                                                                                                               tgctAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCg             >  2:753057/1‑140 (MQ=255)                                                                                                                 ctAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACa                       >  2:260397/1‑128 (MQ=255)                                                                                                                 ctAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACa                       <  1:260397/128‑1 (MQ=255)                                                                                                                 ctAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGc           >  2:802789/1‑140 (MQ=255)                                                                                                                 ctAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGc           >  2:537896/1‑140 (MQ=255)                                                                                                                  tAAGCTGGCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCa          >  2:101469/1‑140 (MQ=255)                                                                                                                         gCAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATg   >  2:143098/1‑140 (MQ=255)                                                                                                                          cAATGTGACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGa  <  1:390713/140‑1 (MQ=255)                                                                                                                              gtgACAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCgg            <  1:220460/126‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     AACCCGTACTGGAAAAAACTGCACGAACACATTGATTCCCGTACCGTCGATAACTGGTGTGATGACCACGATCAAATCCTCAAGGCGCTGATTCGCAAAGATCCTCATGCTGCTAAGCTGGCAATGTGGCAGCATCTGGAAAACACCAAGATCATGTTATTTAACGAAACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGA  >  NC_000913/3247168‑3247428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑