Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,273,700	C→T	G403D (GGC→GAC)	garP ←	galactarate/glucarate/glycerate transporter GarP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,273,700	0	C	T	100.0%	183.9 / NA	53	G403D (GGC→GAC)	garP	galactarate/glucarate/glycerate transporter GarP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/21);  total (32/21)

ACCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAA  >  NC_000913/3273565‑3273832                                                                                                                                        |                                                                                                                                     aCCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAAt                                                                                                                                  <  1:603426/140‑1 (MQ=255)    tttGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCAc                                                                                                                               <  2:266348/140‑1 (MQ=255)    tttGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCAc                                                                                                                               <  1:635582/140‑1 (MQ=255)    tttGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCAc                                                                                                                               >  2:174075/1‑140 (MQ=255)      tGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCTcc                                                                                                                              <  2:767175/139‑1 (MQ=255)      tGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCAcc                                                                                                                              >  1:767175/1‑139 (MQ=255)      tGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCa                                                                                                                             >  2:641104/1‑140 (MQ=255)       gCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAg                                                                                                                            >  2:756958/1‑140 (MQ=255)       gCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAg                                                                                                                            <  1:788337/140‑1 (MQ=255)               cTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGa                                                                                                                    <  2:209144/140‑1 (MQ=255)               cTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGa                                                                                                                    <  1:174075/140‑1 (MQ=255)                  cAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACaa                                                                                                                 >  2:230237/1‑140 (MQ=255)                               ttAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACAtt                                                                                                    >  1:823906/1‑140 (MQ=255)                                 aaTGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGc                                                                                                  >  2:8649/1‑140 (MQ=255)                                  aTGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGcc                                                                                                 <  1:843252/140‑1 (MQ=255)                                     tCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCaaa                                                                                              <  1:756958/140‑1 (MQ=255)                                     tCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCaaa                                                                                              <  1:8649/140‑1 (MQ=255)                                      cGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAg                                                                                             >  2:626712/1‑140 (MQ=255)                                       gCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGcc                                                                                                 <  1:467224/135‑1 (MQ=255)                                       gCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGcc                                                                                                 >  2:467224/1‑135 (MQ=255)                                       gCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGa                                                                                            >  1:738439/1‑140 (MQ=255)                                                          cAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCa                                                                         >  1:94160/1‑140 (MQ=255)                                                              ccATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGa                                                                                   >  1:748503/1‑126 (MQ=255)                                                              ccATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGa                                                                                   <  2:748503/126‑1 (MQ=255)                                                              ccATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAgg                                                                     >  2:676520/1‑140 (MQ=255)                                                              ccATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAgg                                                                     >  1:128318/1‑140 (MQ=255)                                                               cATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGc                                                                    >  2:430162/1‑140 (MQ=255)                                                               cATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGc                                                                    >  2:290003/1‑140 (MQ=255)                                                                aTCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGcc                                                                   >  1:276867/1‑140 (MQ=255)                                                                 tCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCca                                                                  >  2:624461/1‑140 (MQ=255)                                                                 tCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCca                                                                  >  1:172705/1‑140 (MQ=255)                                                                  cGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCcaa                                                                 >  2:50366/1‑140 (MQ=255)                                                                                   aTCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGcgcg                                                <  2:94160/140‑1 (MQ=255)                                                                                      ccACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGgtg                                             <  2:172705/140‑1 (MQ=255)                                                                                         cGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCa                                          >  1:482501/1‑140 (MQ=255)                                                                                          gAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGG‑GGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGa                                        >  2:349310/1‑140 (MQ=255)                                                                                               ccAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATc                                    <  1:50366/140‑1 (MQ=255)                                                                                                cAGTGCTGCATTGAAGGAGTGCAGTACACTTACCAGGTAGTCAAGCTCCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCa                                   <  2:128318/140‑1 (MQ=255)                                                                                                     ctgcATTGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGc                              <  2:738439/140‑1 (MQ=255)                                                                                                          ttGAAGGAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGccagcc                         <  2:713518/140‑1 (MQ=255)                                                                                                               ggAGTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAgcg                    >  1:760374/1‑140 (MQ=255)                                                                                                                  gTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCa                                   <  2:422198/122‑1 (MQ=255)                                                                                                                  gTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCa                                   >  1:422198/1‑122 (MQ=255)                                                                                                                  gTGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGG‑GGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCAcc                <  1:349310/140‑1 (MQ=255)                                                                                                                   tGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCAcc                >  2:530897/1‑140 (MQ=255)                                                                                                                   tGCAGTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCAcc                >  2:447759/1‑140 (MQ=255)                                                                                                                       gTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAAt            >  2:166586/1‑140 (MQ=255)                                                                                                                       gTTCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAAt            >  2:25472/1‑140 (MQ=255)                                                                                                                        ttCACTTACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATc           >  1:580773/1‑140 (MQ=255)                                                                                                                             ttACCAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTg      >  1:494454/1‑140 (MQ=255)                                                                                                                                ccAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCa   <  1:530897/140‑1 (MQ=255)                                                                                                                                 cAGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCaa  >  2:445390/1‑140 (MQ=255)                                                                                                                                  aGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCt         <  2:430682/132‑1 (MQ=255)                                                                                                                                  aGGTAGTCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCt         >  1:430682/1‑132 (MQ=255)                                                                                                                                        |                                                                                                                                     ACCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAA  >  NC_000913/3273565‑3273832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑