Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,487	C→T	intergenic (‑437/‑238)	yhcC ← / → gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,487	0	C	T	100.0%	170.8 / NA	48	intergenic (‑437/‑238)	yhcC/gltB	radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

GTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGA  >  NC_000913/3354351‑3354622                                                                                                                                         |                                                                                                                                        gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                                      >  2:750508/1‑140 (MQ=255) gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                                      >  1:166048/1‑140 (MQ=255) gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                                      >  1:224115/1‑140 (MQ=255) gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                                      >  2:100724/1‑140 (MQ=255) gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGc                                                                                                                                      >  1:316841/1‑140 (MQ=255)    aGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCttt                                                                                                                                   >  2:109114/1‑140 (MQ=255)                  tctcTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGa                                                                                                                     >  2:839448/1‑140 (MQ=255)                     ctAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAAt                                                                                                                  <  1:750508/140‑1 (MQ=255)                             ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                                          <  1:839448/140‑1 (MQ=255)                             ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                                          >  1:313531/1‑140 (MQ=255)                             ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                                          >  1:461253/1‑140 (MQ=255)                             ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                                          >  2:437729/1‑140 (MQ=255)                             ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                                          <  2:316841/140‑1 (MQ=255)                                  ttttAGCCTTAAAGATAAGATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCtt                                                                                                     >  2:828884/1‑140 (MQ=255)                                  ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCtt                                                                                                     <  1:440812/140‑1 (MQ=255)                                  ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCtt                                                                                                     <  2:224115/140‑1 (MQ=255)                                  ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCtt                                                                                                     <  2:234327/140‑1 (MQ=255)                                          ttAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATg                                                                                             >  2:214442/1‑140 (MQ=255)                                                aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTATCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGagcagc                                                                                       <  1:258227/140‑1 (MQ=255)                                                aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGagcagc                                                                                       <  2:353228/140‑1 (MQ=255)                                                  aaaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCt                                                                                     >  1:404858/1‑140 (MQ=255)                                                   aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                    <  2:461253/140‑1 (MQ=255)                                                   aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                    <  2:521507/140‑1 (MQ=255)                                                   aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                    <  1:100724/140‑1 (MQ=255)                                                   aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                    <  1:437729/140‑1 (MQ=255)                                                     aTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                  <  1:214442/140‑1 (MQ=255)                                                     aTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                  <  1:510188/140‑1 (MQ=255)                                                     aTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                  <  2:283095/140‑1 (MQ=255)                                                               aTTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATAttt                                                                        >  2:237230/1‑140 (MQ=255)                                                               aTTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATAttt                                                                        >  2:639109/1‑140 (MQ=255)                                                                tttCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTa                                                                       <  2:191107/140‑1 (MQ=255)                                                                  tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                     <  1:828884/140‑1 (MQ=255)                                                                  tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                     <  1:755827/140‑1 (MQ=255)                                                                   cAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                    <  1:494178/140‑1 (MQ=255)                                                                                 aGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                    <  1:783359/126‑1 (MQ=255)                                                                                 aGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                    >  2:783359/1‑126 (MQ=255)                                                                                   aaTTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATcccc                                                    >  2:678745/1‑140 (MQ=255)                                                                                    aTTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCct                                                   >  1:793357/1‑140 (MQ=255)                                                                                           gcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCAcc                                            <  1:639109/140‑1 (MQ=255)                                                                                           gcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCAcc                                            <  1:237230/140‑1 (MQ=255)                                                                                                          tGTACCAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCg                             >  2:789210/1‑140 (MQ=255)                                                                                                              ccAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaaaa                         <  1:109114/140‑1 (MQ=255)                                                                                                               cAATAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAg                        >  2:140533/1‑140 (MQ=255)                                                                                                                 aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaa                           >  2:66217/1‑135 (MQ=255)                                                                                                                 aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaa                           <  1:66217/135‑1 (MQ=255)                                                                                                                 aTAAGCTTGCCATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa                      <  2:793357/140‑1 (MQ=255)                                                                                                                          ccATTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggg             >  2:701849/1‑140 (MQ=255)                                                                                                                                     gTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa  >  1:668202/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGA  >  NC_000913/3354351‑3354622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑