Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,628	G→A	G241G (GGC→GGT)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,628	0	G	A	100.0%	127.8 / NA	39	G241G (GGC→GGT)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

TGGGCCAACAGCGCAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTGCCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGGCGCGCGTGAAAATAGTTTT  >  NC_000913/3369491‑3369762                                                                                                                                          |                                                                                                                                       tGGGCCAACAGCGCAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTAcc                                                                                                                                      >  2:398981/1‑140 (MQ=255)      cAACAGCGCAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGa                                                                                                                                 <  1:687117/140‑1 (MQ=255)             gcAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACcaccac                                                                                                                          >  1:162876/1‑140 (MQ=255)              cAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCCGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACg                                                                                                                         >  1:411569/1‑140 (MQ=255)              cAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACcaccacc                                                                                                                         >  2:115685/1‑139 (MQ=255)              cAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACg                                                                                                                         >  1:40402/1‑140 (MQ=255)              cAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACg                                                                                                                         >  2:371272/1‑140 (MQ=255)                gCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCa                                                                                                                       <  1:76598/140‑1 (MQ=255)                        tAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAAt                                                                                                               <  1:371272/140‑1 (MQ=255)                        tAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAAt                                                                                                               <  1:398981/140‑1 (MQ=255)                           aCCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCa                                                                                                                       >  2:591393/1‑129 (MQ=255)                           aCCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCa                                                                                                                       <  1:591393/129‑1 (MQ=255)                           aCCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAg                                                                                                            >  1:360081/1‑140 (MQ=255)                              tGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTtg                                                                                                         <  2:40402/140‑1 (MQ=255)                                catcatGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTtgtg                                                                                                       <  2:60892/140‑1 (MQ=255)                                catcatGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTtgtg                                                                                                       <  2:360081/140‑1 (MQ=255)                                catcatGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTtgtg                                                                                                       <  2:162876/140‑1 (MQ=255)                                          gTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAatat                                                                                             >  1:791034/1‑140 (MQ=255)                                               gcgcCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCg                                                                                        <  1:518756/140‑1 (MQ=255)                                                    gccAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAg                                                                                   <  2:245218/140‑1 (MQ=255)                                                                aCATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGt                                                                       <  2:751581/140‑1 (MQ=255)                                                                aCATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGt                                                                       <  2:411569/140‑1 (MQ=255)                                                                       aTGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCg                                                                <  1:107285/140‑1 (MQ=255)                                                                           cGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGACCAGCCTTGCAAGCGTACGTGCGGAGc                                                            <  1:742828/140‑1 (MQ=255)                                                                            gCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCg                                                           <  2:12393/140‑1 (MQ=255)                                                                              tGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCgg                                                               >  2:144290/1‑134 (MQ=255)                                                                              tGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCgg                                                               <  1:144290/134‑1 (MQ=255)                                                                                gCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTAc                                                                     >  2:102163/1‑126 (MQ=255)                                                                                gCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTAc                                                                     <  1:102163/126‑1 (MQ=255)                                                                                                  tttCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGc                                     <  2:791034/140‑1 (MQ=255)                                                                                                   ttCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCt                                    >  2:147618/1‑140 (MQ=255)                                                                                                      caccaGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGt                                 >  1:853874/1‑140 (MQ=255)                                                                                                      caccaGCGCCAGATACCCTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGt                                 >  1:141561/1‑140 (MQ=255)                                                                                                                      ccTTCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGgcgcg                 >  2:275841/1‑140 (MQ=255)                                                                                                                         tCTGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGGCGCGCGt              >  1:573232/1‑140 (MQ=255)                                                                                                                           tGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGgcg                   >  2:341224/1‑133 (MQ=255)                                                                                                                           tGCCAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGgcg                   <  1:341224/133‑1 (MQ=255)                                                                                                                             ccAGACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGGCGCGCGTGaaa          >  1:569531/1‑140 (MQ=255)                                                                                                                                gACCAACGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGGCGCGCGTGAAAATa       >  1:303052/1‑140 (MQ=255)                                                                                                                                     aCGCTACCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGGCGCGCGTGAAAATAGtttt  <  1:752594/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TGGGCCAACAGCGCAGCCCCAAGTAAACCTGCATCATGGCGGTAATGCGCCGCCAGTAAATCAACATGAAATGCCGCTGGCTCCTGCGCCAGATACGTTTCCACCAGCGCCAGATACCCTTCTGCCAGACCAACGCTGCCACCGACCACCACGCACTGGCAATCAGTTGTGGCTTTAATATCAGCGATCAGCCTTGCAAGCGTACGTGCGGAGCGGTGAATCAGCTGCTGCGCCTGCTCGTCACCCTGCCCGGCGCGCGTGAAAATAGTTTT  >  NC_000913/3369491‑3369762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑