Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,413,892 T→C L10P (CTG→CCG)  acrE → multidrug efflux pump membrane fusion lipoprotein AcrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,413,8920TC100.0% 147.2 / NA 42L10P (CTG→CCG) acrEmultidrug efflux pump membrane fusion lipoprotein AcrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/27);  total (15/27)

ATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGA  >  NC_000913/3413756‑3414024
                                                                                                                                        |                                                                                                                                    
aTCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAATATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   <  2:502233/140‑1 (MQ=255)
aTCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   >  2:339253/1‑140 (MQ=255)
aTCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   >  2:151838/1‑140 (MQ=255)
aTCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   <  1:339253/140‑1 (MQ=255)
aTCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   <  2:453586/140‑1 (MQ=255)
   gacgaATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCcctc                                                                                                                                <  2:568990/140‑1 (MQ=255)
    acgaATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAGCGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCcctcc                                                                                                                               >  2:466590/1‑140 (MQ=255)
    acgaATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCcctcc                                                                                                                               >  2:137797/1‑140 (MQ=255)
     cgaATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCcctcct                                                                                                                              <  2:208945/140‑1 (MQ=255)
     cgaATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCcctcct                                                                                                                              <  1:47859/140‑1 (MQ=255)
       aaTGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   <  1:515634/133‑1 (MQ=255)
       aaTGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGcc                                                                                                                                   >  2:515634/1‑133 (MQ=255)
                    aGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCcg                                                                                                               <  1:732045/140‑1 (MQ=255)
                        tAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGc                                                                                                           >  1:601873/1‑140 (MQ=255)
                         aGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCt                                                                                                          >  1:598794/1‑140 (MQ=255)
                          gCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCtt                                                                                                         >  2:46728/1‑140 (MQ=255)
                             aaCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTCTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTaa                                                                                                      >  2:422334/1‑140 (MQ=255)
                                tGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCg                                                                                                   >  2:65125/1‑140 (MQ=255)
                                        tctttctGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGt                                                                                           <  1:491196/140‑1 (MQ=255)
                                        tctttctGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGt                                                                                           <  2:601873/140‑1 (MQ=255)
                                         ctttctGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTa                                                                                          <  1:46728/140‑1 (MQ=255)
                                                     tAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGgag                                                                              <  2:61737/140‑1 (MQ=255)
                                                      aaCGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGgaga                                                                             >  1:770828/1‑140 (MQ=255)
                                                             tGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGaaa                                                                      >  2:712313/1‑140 (MQ=255)
                                                                  ttttGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCa                                                                 <  1:378348/140‑1 (MQ=255)
                                                                       ggTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCg                                                            <  1:712313/140‑1 (MQ=255)
                                                                          aaaTAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTg                                                         <  2:836568/140‑1 (MQ=255)
                                                                            aTAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCgg                                                           >  2:135694/1‑136 (MQ=255)
                                                                            aTAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCgg                                                           <  1:135694/136‑1 (MQ=255)
                                                                              aaCGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAAcc                                                     <  2:143009/140‑1 (MQ=255)
                                                                              aaCGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAAcc                                                     <  1:694420/140‑1 (MQ=255)
                                                                               aCGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCg                                                    <  1:466590/140‑1 (MQ=255)
                                                                                 gcgcTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCa                                                  <  2:122739/140‑1 (MQ=255)
                                                                                     ttttGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGtt                                              <  1:573667/140‑1 (MQ=255)
                                                                                     ttttGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGtt                                              <  1:137797/140‑1 (MQ=255)
                                                                                      tttGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTa                                             <  2:525318/140‑1 (MQ=255)
                                                                                             ttttGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCa                                      <  2:182308/140‑1 (MQ=255)
                                                                                             ttttGAGGAATAGTAATCACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCa                                      <  2:770828/140‑1 (MQ=255)
                                                                                                    gAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTa                               >  1:203526/1‑140 (MQ=255)
                                                                                                            aTGACGAAACATGCCAGGTTTTTCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGc                       <  1:151838/140‑1 (MQ=255)
                                                                                                                    tcATGCCAGGTTTTTCCTCCCGCCCTCCTTTACTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGTTTACCGTTCATATTGTAAAAACGGCCCCGTTAg               <  2:567497/139‑1 (MQ=255)
                                                                                                                               ttttCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACt    >  1:625941/1‑140 (MQ=255)
                                                                                                                                 ttCCTCCCGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGa  <  2:598794/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
ATCGACGAATGATAATTTGTAGGATAGCGAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTTTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAAGGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGA  >  NC_000913/3413756‑3414024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑