Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,874	C→T	P28P (CCC→CCT)	yhfK →	putative transporter YhfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,874	0	C	T	100.0%	173.5 / NA	50	P28P (CCC→CCT)	yhfK	putative transporter YhfK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/25);  total (25/25)

CACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGT  >  NC_000913/3486743‑3487010                                                                                                                                    |                                                                                                                                         cACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGt                                                                                                                                  <  1:692757/140‑1 (MQ=255)              cGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCtt                                                                                                                     >  2:122217/1‑140 (MQ=255)                tCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGt                                                                                                                                  <  1:605232/125‑1 (MQ=255)                tCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGt                                                                                                                                  >  2:605232/1‑125 (MQ=255)                    aGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGaa                                                                                                               >  1:161557/1‑140 (MQ=255)                            gTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGAtt                                                                                                       <  1:122217/140‑1 (MQ=255)                                 cTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTc                                                                                                  <  2:63181/140‑1 (MQ=255)                                       gcgcgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGctct                                                                                            <  2:161557/140‑1 (MQ=255)                                        cgcgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtctt                                                                                           >  1:542124/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtcttct                                                                                         >  2:743524/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtcttct                                                                                         >  1:290373/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtcttct                                                                                         >  1:809214/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtcttct                                                                                         >  2:260897/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGACTGTGGTCGTTGGGTTAATGCTTGGCGAATTAAGATTCGGTTTGCtgttct                                                                                         >  2:391163/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCACGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtcttct                                                                                         >  2:740099/1‑140 (MQ=255)                                          cgcCATTTTGTTTCCCCCGATGTGGCGCAGAATGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCtcttct                                                                                         >  2:41528/1‑140 (MQ=255)                                            ccATCTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTcc                                                                                       >  1:339249/1‑140 (MQ=255)                                                       cccccGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgc                                                                            >  2:303890/1‑140 (MQ=255)                                                        ccccGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcc                                                                           <  1:63495/140‑1 (MQ=255)                                                        ccccGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTACGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcc                                                                           <  1:789084/140‑1 (MQ=255)                                                         cccGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcct                                                                          >  2:91362/1‑140 (MQ=255)                                                          ccGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcctg                                                                         <  1:303890/140‑1 (MQ=255)                                                          ccGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcctg                                                                         <  2:339249/140‑1 (MQ=255)                                                               gtgGCGCAGACTGATTTATCACCACGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCa                                                                    <  1:740099/140‑1 (MQ=255)                                                                 ggCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAat                                                                  >  2:393697/1‑140 (MQ=255)                                                                     cAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACACACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTg                                                              <  2:809214/140‑1 (MQ=255)                                                                      aGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGc                                                             <  2:290373/140‑1 (MQ=255)                                                                       gACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCg                                                            <  2:572382/140‑1 (MQ=255)                                                                       gACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCg                                                            <  1:260897/140‑1 (MQ=255)                                                                        aCTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcctg                                                                         >  1:377557/1‑126 (MQ=255)                                                                        aCTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgcctg                                                                         <  2:377557/126‑1 (MQ=255)                                                                          tGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGc                                                         <  2:507962/140‑1 (MQ=255)                                                                           gATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGcc                                                        <  2:95744/140‑1 (MQ=255)                                                                                  cACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATAc                                                 >  1:662766/1‑140 (MQ=255)                                                                                      ccGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCt                                             >  1:183765/1‑140 (MQ=255)                                                                                         atatCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCAt                                          >  1:246257/1‑140 (MQ=255)                                                                                                   tGCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGttttt                                >  1:837209/1‑140 (MQ=255)                                                                                                    gCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGtttttt                               >  2:627719/1‑140 (MQ=255)                                                                                                    gCACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGtttttt                               >  1:500461/1‑140 (MQ=255)                                                                                                     cACTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTc                              >  1:558632/1‑140 (MQ=255)                                                                                                      aCTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCa                             <  1:391163/140‑1 (MQ=255)                                                                                                       cTTCGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCaa                            <  1:743524/140‑1 (MQ=255)                                                                                                          cGACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACg                         >  1:254193/1‑140 (MQ=255)                                                                                                           gACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGc                        <  2:558632/140‑1 (MQ=255)                                                                                                           gACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGc                        <  1:393697/140‑1 (MQ=255)                                                                                                           gACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGc                        <  2:254193/140‑1 (MQ=255)                                                                                                           gACAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGc                        <  1:91362/140‑1 (MQ=255)                                                                                                             cAAACGCTGGTGCTATGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCtt                      <  1:348730/140‑1 (MQ=255)                                                                                                                           aTGTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGt        >  2:677897/1‑140 (MQ=255)                                                                                                                             gTTTGCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCg      <  1:227471/140‑1 (MQ=255)                                                                                                                                 gCCTGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGt  >  2:649451/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         CACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACGTTTTTTCAAACGCTTAATCATTGGTGCGTCGCTGT  >  NC_000913/3486743‑3487010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑