Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,590,205	G→A	A236V (GCC→GTC)	ugpA ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,590,205	0	G	A	100.0%	182.2 / NA	52	A236V (GCC→GTC)	ugpA	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/20);  total (32/20)

GCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCG  >  NC_000913/3590074‑3590339                                                                                                                                    |                                                                                                                                       gCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTc                                                                                                                                    >  1:773466/1‑136 (MQ=255) gCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTc                                                                                                                                    <  2:867182/136‑1 (MQ=255) gCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTc                                                                                                                                    <  2:773466/136‑1 (MQ=255) gCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTc                                                                                                                                    >  1:867182/1‑136 (MQ=255)        gACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGa                                                                                                                         <  2:482364/140‑1 (MQ=255)                 cATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCga                                                                                                                >  2:219839/1‑140 (MQ=255)                   tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCgaag                                                                                                              >  2:455470/1‑140 (MQ=255)                   tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCgaag                                                                                                              <  1:219839/140‑1 (MQ=255)                   tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCgaag                                                                                                              <  1:455470/140‑1 (MQ=255)                         ccaccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCa                                                                                                        >  1:692830/1‑140 (MQ=255)                         ccaccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCa                                                                                                        <  2:13727/140‑1 (MQ=255)                            ccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCgaag                                                                                                              <  2:33102/131‑1 (MQ=255)                            ccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCgaag                                                                                                              >  1:33102/1‑131 (MQ=255)                            ccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAATGCATac                                                                                                     >  2:737241/1‑140 (MQ=255)                             cGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATaca                                                                                                    <  2:279488/140‑1 (MQ=255)                               aCTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACAcc                                                                                                  >  1:584222/1‑140 (MQ=255)                               aCTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACAcc                                                                                                  >  1:790502/1‑140 (MQ=255)                               aCTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACAcc                                                                                                  >  2:753956/1‑140 (MQ=255)                                    gCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGtt                                                                                             >  2:635784/1‑140 (MQ=255)                                          gACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTAc                                                                                       >  1:420827/1‑140 (MQ=255)                                           aCGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTAcc                                                                                      >  1:295603/1‑140 (MQ=255)                                               aGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACcagca                                                                                  >  1:331694/1‑140 (MQ=255)                                                gCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACcagcag                                                                                 >  1:172103/1‑140 (MQ=255)                                                          gTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTc                                                                       >  1:705071/1‑140 (MQ=255)                                                               gTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCgg                                                                  >  1:181795/1‑140 (MQ=255)                                                               gTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCgg                                                                  >  1:452086/1‑140 (MQ=255)                                                                           cgGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGTAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCgg                                                      <  2:134943/140‑1 (MQ=255)                                                                                gATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                 <  2:705071/140‑1 (MQ=255)                                                                                   cTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCaa                                              <  2:496341/140‑1 (MQ=255)                                                                                           tCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAaag                                      >  2:8540/1‑140 (MQ=255)                                                                                            cAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCTATAAGCGGCAGCGCAATCTTAaaga                                     >  1:664543/1‑140 (MQ=255)                                                                                            cAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                >  1:730750/1‑129 (MQ=255)                                                                                            cAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                <  2:730750/129‑1 (MQ=255)                                                                                             aGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGGGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAaagaa                                    >  1:491781/1‑140 (MQ=255)                                                                                              gCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAaagaag                                   >  1:76261/1‑140 (MQ=255)                                                                                               cgtcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                  >  1:725711/1‑140 (MQ=255)                                                                                               cgtcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                  >  1:610648/1‑140 (MQ=255)                                                                                               cgtcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                  <  2:76261/140‑1 (MQ=255)                                                                                                 tcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCTATAAGCGGCAGCGCAATCTTAAAGAAgcgg                                <  2:664543/140‑1 (MQ=255)                                                                                                     ggtggCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGaa                            >  1:173423/1‑140 (MQ=255)                                                                                                      gtggCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                           <  2:452086/140‑1 (MQ=255)                                                                                                      gtggCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                           <  2:181795/140‑1 (MQ=255)                                                                                                      gtggCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                           <  1:753956/140‑1 (MQ=255)                                                                                                         gCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCgg                        >  2:454757/1‑140 (MQ=255)                                                                                                         gCCTGAACCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCgg                        >  1:722627/1‑140 (MQ=255)                                                                                                              aaCCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                           >  2:342803/1‑132 (MQ=255)                                                                                                              aaCCGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                           <  1:342803/132‑1 (MQ=255)                                                                                                                ccGGCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCa                 <  2:725711/140‑1 (MQ=255)                                                                                                                   gCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCgg                        >  2:646785/1‑130 (MQ=255)                                                                                                                   gCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCgg                        <  1:646785/130‑1 (MQ=255)                                                                                                                   gCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCg              >  1:216302/1‑140 (MQ=255)                                                                                                                   gCCCGCCGGACGTGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCg              >  1:4608/1‑140 (MQ=255)                                                                                                                               tGGCGACGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCg  <  1:635784/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCG  >  NC_000913/3590074‑3590339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑