Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,778,503	T→C	F369L (TTC→CTC)	lldP →	lactate/glycolate:H(+) symporter LldP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,778,503	0	T	C	100.0%	110.1 / NA	33	F369L (TTC→CTC)	lldP	lactate/glycolate:H(+) symporter LldP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/4);  total (29/4)

TGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTA  >  NC_000913/3778369‑3778638                                                                                                                                       |                                                                                                                                        tGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGctctct                                                                                                                                    <  2:402668/140‑1 (MQ=255) tGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGctctct                                                                                                                                    >  1:755135/1‑140 (MQ=255)  gTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTg                                                                                                                                   >  1:202563/1‑140 (MQ=255)             gTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCAcc                                                                                                                        >  1:59655/1‑140 (MQ=255)                      tGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTg                                                                                                               >  2:658351/1‑140 (MQ=255)                                ggTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCAc                                                                                                     >  1:327151/1‑140 (MQ=255)                                ggTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCAc                                                                                                     >  1:764610/1‑140 (MQ=255)                                 gTGATCAATATTCCGGTGCCGTCCCTCAAAAACCGGTTTGCCCGTATGCCGCCAGTGGCCAGCGAGGCTACAGCCTATGCCGCGGTGTTTAAGTTTGACTGGCTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACt                                                                                                    <  2:755135/140‑1 (MQ=255)                                   gATCAATTTTCCGGTGCCGTCCCTCGAAAACTTGGTTCCCCTTATGCCGCCAGTGGTCGGCGGGGCTACAGCCTATGCCGCCGTTTTTAAGTTTGACTGGCTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGc                                                                                                  <  2:59655/140‑1 (MQ=255)                                   gATAAATTTCCCGGTCCCTTCCCTCAAAAACTTGTTTCCCCGTATGCCGCCGGTGGCCAGCGAGGCTACACCCTATCCCGCCGTTTTTAATTTTGACTGGCTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGc                                                                                                  <  2:360876/140‑1 (MQ=255)                                           ttCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGAtt                                                                                          >  1:865855/1‑140 (MQ=255)                                            tCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTg                                                                                         >  1:360876/1‑140 (MQ=255)                                               ggTGCCGTCCCTCGAAAAACTGTTGCCCCGTATCCCGCCAGTGGTCGGCGAGCCTACACCCTATGCCGCCGTTTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCt                                                                                      <  2:70067/140‑1 (MQ=255)                                                gTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTg                                                                                     >  2:773734/1‑140 (MQ=255)                                                gTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTg                                                                                     >  1:54434/1‑140 (MQ=255)                                                      tACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGaa                                                                               >  1:70067/1‑140 (MQ=255)                                                      tACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGaa                                                                               >  2:208490/1‑140 (MQ=255)                                                           cGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGa                                                                          >  2:473745/1‑140 (MQ=255)                                                           cGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGa                                                                          >  2:603947/1‑140 (MQ=255)                                                           cGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGa                                                                          >  1:88846/1‑140 (MQ=255)                                                                aaCTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCg                                                                     >  1:547335/1‑140 (MQ=255)                                                                     gTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGa                                                                >  1:444891/1‑140 (MQ=255)                                                                      ggcccggTATGCCGCCGGGGGCCGGCGGGCCTACAGCCTTTCCCGCGGTTTTTAAGTTTGACGGGCTCTCCGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGAc                                                               <  2:211887/134‑1 (MQ=255)                                                                               tGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGc                                                      >  1:345599/1‑140 (MQ=255)                                                                                   gccAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGAc                                                               <  2:289910/127‑1 (MQ=255)                                                                                   gccAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGAc                                                               >  1:289910/1‑127 (MQ=255)                                                                                   gccAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCt                                                  >  1:211887/1‑140 (MQ=255)                                                                                      aGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCg                                               >  1:215078/1‑140 (MQ=255)                                                                                       gTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCgg                                              >  1:538372/1‑140 (MQ=255)                                                                                          gTCGGCGGGGCTACAGCCTATCCCGCCGTTTTTAAGTTTGACTGGCTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGgcag                                           <  2:88846/140‑1 (MQ=255)                                                                                              gCGAGGCTACAGCCTATCCCGCCGTGTTTAAGTTTGACTGGCTCTCTCCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACg                                       <  2:4228/140‑1 (MQ=255)                                                                                               cGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGc                                      >  2:267683/1‑140 (MQ=255)                                                                                                        aGCCTATGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAAc                             <  1:473745/140‑1 (MQ=255)                                                                                                            tATGCCGCCGTTTTTAATTTTGACTGGCTCTCTGCCCCGGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGc                         <  2:538372/140‑1 (MQ=255)                                                                                                             aTGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCAc                                        <  1:667275/124‑1 (MQ=255)                                                                                                             aTGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCAc                                        >  2:667275/1‑124 (MQ=255)                                                                                                             aTGCCGCCGTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGct                        >  2:132325/1‑140 (MQ=255)                                                                                                              ggccgccGTTTTTAATTTTGACTGGCTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGctc                       <  2:444891/139‑1 (MQ=255)                                                                                                                ccgccgTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTg                     <  1:331019/140‑1 (MQ=255)                                                                                                                cccccgTTTTTAATTTTGCCTGGCTCCCGCCCCCCGGAACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACCGGCTCTg                     <  2:547335/137‑1 (MQ=255)                                                                                                                  gccgTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGcc                   >  1:718992/1‑140 (MQ=255)                                                                                                                    cgTGTTTAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCa                 >  2:565334/1‑140 (MQ=255)                                                                                                                        tttAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTa             <  2:718992/140‑1 (MQ=255)                                                                                                                         ttAAGTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTAc            <  1:603947/140‑1 (MQ=255)                                                                                                                             gTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCa        <  1:658351/140‑1 (MQ=255)                                                                                                                             gTTTGACTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCa        >  2:233171/1‑140 (MQ=255)                                                                                                                                  aCTGGCTCTCTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGt   <  1:213313/140‑1 (MQ=255)                                                                                                                                  aCTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGc                    <  1:720176/123‑1 (MQ=255)                                                                                                                                  aCTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGc                    >  2:720176/1‑123 (MQ=255)                                                                                                                                  aCTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGt   >  1:811669/1‑140 (MQ=255)                                                                                                                                   cTGGCTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTa  <  2:811669/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTTGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTA  >  NC_000913/3778369‑3778638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑