Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,533	T→C	intergenic (‑78/+12)	mioC ← / ← asnC	flavoprotein MioC/DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,533	0	T	C	100.0%	201.0 / NA	57	intergenic (‑78/+12)	mioC/asnC	flavoprotein MioC/DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGCTGGAGAGCGTCGATCGAA  >  NC_000913/3926401‑3926662                                                                                                                                     |                                                                                                                                  cTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAg                                                                                                                            <  1:678329/140‑1 (MQ=255) cTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAg                                                                                                                            <  2:667431/140‑1 (MQ=255)    cATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCg                                                                                                                         >  1:505489/1‑140 (MQ=255)      tatTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGAt                                                                                                                       >  1:463080/1‑140 (MQ=255)        ttCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCa                                                                                                                     <  1:684623/140‑1 (MQ=255)           ggCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAggg                                                                                                                  <  1:684177/140‑1 (MQ=255)           ggCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCATATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAggg                                                                                                                  >  1:672912/1‑140 (MQ=255)            gCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGc                                                                                                                 <  2:722545/140‑1 (MQ=255)             cACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCt                                                                                                                <  2:60244/140‑1 (MQ=255)                  ccgAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCATATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATg                                                                                                           <  2:672912/140‑1 (MQ=255)                       ggTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACg                                                                                                      >  1:422102/1‑140 (MQ=255)                       ggTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACg                                                                                                      <  2:505489/140‑1 (MQ=255)                         tgctgcCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCa                                                                                                    <  2:230746/140‑1 (MQ=255)                             gcCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGAt                                                                                                >  2:41801/1‑140 (MQ=255)                                 cTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCggg                                                                                            >  2:313716/1‑140 (MQ=255)                                 cTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCggg                                                                                            >  1:324755/1‑140 (MQ=255)                                      aaGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTg                                                                                       <  2:257841/140‑1 (MQ=255)                                       agagTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGc                                                                                      <  2:9307/140‑1 (MQ=255)                                             atatCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACg                                                                                >  2:245573/1‑140 (MQ=255)                                                   gCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAAt                                                                          >  1:348400/1‑140 (MQ=255)                                                   gCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAAt                                                                          >  1:347055/1‑140 (MQ=255)                                                   gCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAAt                                                                          >  1:224335/1‑140 (MQ=255)                                                            cGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAGTGTCTCGGTg                                                                 >  2:155625/1‑140 (MQ=255)                                                              ccACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGa                                                               <  2:448407/140‑1 (MQ=255)                                                              ccACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGa                                                               >  1:463830/1‑140 (MQ=255)                                                              ccACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGa                                                               <  2:726704/140‑1 (MQ=255)                                                               cACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCATGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGAc                                                              >  1:443689/1‑140 (MQ=255)                                                                      attaAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAAttt                                                       <  2:347055/140‑1 (MQ=255)                                                                          agagTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTtcatc                                                   <  1:313716/140‑1 (MQ=255)                                                                          agagTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTtcatc                                                   <  1:655280/140‑1 (MQ=255)                                                                            agTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTtcatca                                                  <  2:726488/139‑1 (MQ=255)                                                                            agTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTtcatca                                                  >  1:726488/1‑139 (MQ=255)                                                                            agTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCaa                                                 >  2:133858/1‑140 (MQ=255)                                                                            agTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCaa                                                 <  2:348400/140‑1 (MQ=255)                                                                            agTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCaa                                                 <  2:422102/140‑1 (MQ=255)                                                                            agTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCaa                                                 <  2:463830/140‑1 (MQ=255)                                                                              tGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATAGGGTTTTGCAGGACGATCGATGTCTCGGTGGACTGAATTTCATCAAtt                                               >  1:425599/1‑140 (MQ=255)                                                                               ggCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTtcatca                                                  <  2:156901/136‑1 (MQ=255)                                                                               ggCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTtcatca                                                  >  1:156901/1‑136 (MQ=255)                                                                                gCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGt                                             >  1:59294/1‑140 (MQ=255)                                                                                gCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGt                                             >  1:486547/1‑140 (MQ=255)                                                                                 cGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGtt                                            >  2:758942/1‑140 (MQ=255)                                                                                          cGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCttg                                   <  2:224335/140‑1 (MQ=255)                                                                                            cTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCATGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCttgtt                                 <  2:443689/140‑1 (MQ=255)                                                                                               tgAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGAt                              >  2:848912/1‑140 (MQ=255)                                                                                               tgAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGAt                              >  1:95159/1‑140 (MQ=255)                                                                                               tgAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATAGGGTTTTGCAGGACGATCGATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGAt                              <  2:425599/140‑1 (MQ=255)                                                                                                gAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATa                             >  2:726412/1‑140 (MQ=255)                                                                                                   cgcgTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGt                          <  2:463080/140‑1 (MQ=255)                                                                                                   cgcgTTGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGt                          <  2:486547/140‑1 (MQ=255)                                                                                                       ttGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACAt                      >  1:837829/1‑140 (MQ=255)                                                                                                        tGGGATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATg                     <  2:324755/140‑1 (MQ=255)                                                                                                         gggATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGc                    >  2:356030/1‑140 (MQ=255)                                                                                                         gggATCTACCTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGc                    >  1:106475/1‑140 (MQ=255)                                                                                                                  cTGTGGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGCTGGAGAGCg           >  2:488014/1‑140 (MQ=255)                                                                                                                     tgGAAAAGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGCTGGAGAGCGtcg        >  2:850223/1‑140 (MQ=255)                                                                                                                           aGTATGGGATCAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGCTGGAGAGCGTCGATCGaa  <  1:356030/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  CTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTGGGATCTACCTGTGGAAAAGTATGGGATTAAAAAAGCCGATCAGGGCTTGATGGTACGCATGATCGGGTTTTGCAGGACGATCAATGTCTCGGTGGACTGAATTTCATCAATTGTTTGGATCTTGTTGATAAGTACATGCTGGAGAGCGTCGATCGAA  >  NC_000913/3926401‑3926662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑