Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,976,924	A→G	Q216R (CAG→CGG)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,924	0	A	G	100.0%	255.3 / NA	71	Q216R (CAG→CGG)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/41);  total (30/41)

GTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTC  >  NC_000913/3976789‑3977059                                                                                                                                        |                                                                                                                                        gTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTg                                                                                                                                     >  1:588337/1‑140 (MQ=255)            gCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTAc                                                                                                                          >  2:63265/1‑140 (MQ=255)             cGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACg                                                                                                                         >  2:321648/1‑140 (MQ=255)                      gTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCg                                                                                                                <  1:124991/140‑1 (MQ=255)                      gTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCg                                                                                                                <  2:82813/140‑1 (MQ=255)                         tGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTg                                                                                                             >  2:582989/1‑140 (MQ=255)                          ggCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGa                                                                                                            >  1:214338/1‑140 (MQ=255)                           gcgcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGAt                                                                                                           >  2:32802/1‑140 (MQ=255)                             gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTa                                                                                                         <  1:321648/140‑1 (MQ=255)                             gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTa                                                                                                         <  1:63265/140‑1 (MQ=255)                             gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTa                                                                                                         <  1:515555/140‑1 (MQ=255)                             gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTa                                                                                                         <  1:295796/140‑1 (MQ=255)                                  ttCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCg                                                                                                    <  1:582989/140‑1 (MQ=255)                                        cgcTTGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGAcc                                                                                              <  2:788723/140‑1 (MQ=255)                                             gtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGcc                                                                                         <  1:715180/140‑1 (MQ=255)                                             gtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGcc                                                                                         >  1:208844/1‑140 (MQ=255)                                                     tCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGt                                                                                                  <  2:216407/123‑1 (MQ=255)                                                     tCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGt                                                                                                  >  1:216407/1‑123 (MQ=255)                                                     tCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCtt                                                                                 <  2:456283/140‑1 (MQ=255)                                                        tGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAca                                                                              >  1:765414/1‑140 (MQ=255)                                                         gccgccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGc                                                                                          <  1:146574/127‑1 (MQ=255)                                                         gccgccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGc                                                                                          >  2:146574/1‑127 (MQ=255)                                                          ccgccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcatc                                                                            >  1:308946/1‑140 (MQ=255)                                                            gccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcatcat                                                                          <  2:267515/140‑1 (MQ=255)                                                              catcatGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatga                                                                        <  1:216547/140‑1 (MQ=255)                                                               atcatGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGc                                                                                   >  2:253107/1‑128 (MQ=255)                                                               atcatGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGc                                                                                   <  1:253107/128‑1 (MQ=255)                                                               atcatGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatgat                                                                       >  1:23005/1‑140 (MQ=255)                                                               atcatGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatgat                                                                       >  1:829600/1‑140 (MQ=255)                                                                   tGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGt                                                                   <  2:588337/140‑1 (MQ=255)                                                                        aTCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAAct                                                              <  1:824209/140‑1 (MQ=255)                                                                          cAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAActgc                                                            >  1:560939/1‑140 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         >  1:612699/1‑140 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         <  2:49831/140‑1 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         <  2:528512/140‑1 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         >  2:535396/1‑140 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         <  2:23005/140‑1 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         >  1:545779/1‑140 (MQ=255)                                                                             aCGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGCATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                         <  2:765414/140‑1 (MQ=255)                                                                              cGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggc                                                        >  1:856676/1‑140 (MQ=255)                                                                                tggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                      <  2:560939/140‑1 (MQ=255)                                                                                tggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                      <  2:541998/140‑1 (MQ=255)                                                                                tggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                      <  2:535692/140‑1 (MQ=255)                                                                                 ggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcggc                                                     <  2:308946/140‑1 (MQ=255)                                                                                  gtgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                      >  1:790566/1‑138 (MQ=255)                                                                                  gtgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                      <  2:790566/138‑1 (MQ=255)                                                                                  gtgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGgcggcg                                                    >  2:624833/1‑140 (MQ=255)                                                                                  gtgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGgcggcg                                                    <  2:116584/140‑1 (MQ=255)                                                                                   tgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcg                                                       <  2:97933/136‑1 (MQ=255)                                                                                   tgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcg                                                       >  1:97933/1‑136 (MQ=255)                                                                                       aTCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGgcggcg                                                    <  1:364920/135‑1 (MQ=255)                                                                                       aTCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGgcggcg                                                    >  2:364920/1‑135 (MQ=255)                                                                                       aTCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCGGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGta                                               >  2:586814/1‑140 (MQ=255)                                                                                        tCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGtat                                              >  2:786778/1‑140 (MQ=255)                                                                                          ccGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAg                                            <  1:624833/140‑1 (MQ=255)                                                                                                aGCTATCTGAAGCCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGa                                      <  1:786778/140‑1 (MQ=255)                                                                                                aGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGa                                      <  1:516465/140‑1 (MQ=255)                                                                                                aGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGa                                      <  2:129598/140‑1 (MQ=255)                                                                                                aGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGa                                      <  2:172836/140‑1 (MQ=255)                                                                                                 gCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGAt                                     <  2:307675/140‑1 (MQ=255)                                                                                                     tCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAgg                                 >  1:438566/1‑140 (MQ=255)                                                                                                     tCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGACTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAgg                                 <  2:565709/140‑1 (MQ=255)                                                                                                               aGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGAt                          <  1:270583/137‑1 (MQ=255)                                                                                                               aGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGAt                          >  2:270583/1‑137 (MQ=255)                                                                                                               aGCTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTg                       >  1:355952/1‑140 (MQ=255)                                                                                                                 cTGGGATAACGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                     >  2:377738/1‑140 (MQ=255)                                                                                                                         aCGGTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg             <  2:438566/140‑1 (MQ=255)                                                                                                                           ggTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg           <  1:762247/140‑1 (MQ=255)                                                                                                                           ggTCTGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg           <  2:829600/140‑1 (MQ=255)                                                                                                                               tGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg             <  1:41269/134‑1 (MQ=255)                                                                                                                               tGGCAGGGCGGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg             >  2:41269/1‑134 (MQ=255)                                                                                                                                ggcagggcggTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGa            <  2:476474/134‑1 (MQ=255)                                                                                                                                ggcagggcggTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGa            >  1:476474/1‑134 (MQ=255)                                                                                                                                    gggcggTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTc  <  2:355952/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTC  >  NC_000913/3976789‑3977059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑