Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,093	C→T	G388S (GGT→AGT)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,093	0	C	T	100.0%	165.5 / NA	48	G388S (GGT→AGT)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

TTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAG  >  NC_000913/4240962‑4241225                                                                                                                                    |                                                                                                                                     tttGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACa                                                                                                                              >  1:501704/1‑140 (MQ=255) tttGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGCCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACa                                                                                                                              >  2:82303/1‑140 (MQ=255)         tcatcGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAg                                                                                                                      >  2:824684/1‑140 (MQ=255)             gggaaaGGTCCAGGAAAAGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAggcgg                                                                                                                  <  2:196330/136‑1 (MQ=255)                    gTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATa                                                                                                           >  2:574070/1‑140 (MQ=255)                      ccAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGa                                                                                                         <  1:274340/140‑1 (MQ=255)                      ccAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGa                                                                                                         <  1:391827/140‑1 (MQ=255)                         ggAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACa                                                                                                      <  1:137602/140‑1 (MQ=255)                           agaCGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGc                                                                                                    <  1:313514/140‑1 (MQ=255)                           agaCGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGc                                                                                                    <  1:337119/140‑1 (MQ=255)                              cGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATc                                                                                                 >  1:613807/1‑140 (MQ=255)                               gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCGTACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCg                                                                                                >  2:288033/1‑140 (MQ=255)                               gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCg                                                                                                >  2:528875/1‑140 (MQ=255)                               gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCg                                                                                                >  1:310795/1‑140 (MQ=255)                               gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCg                                                                                                >  1:616025/1‑140 (MQ=255)                               gAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCg                                                                                                >  2:586295/1‑140 (MQ=255)                                 agtagtTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGAc                                                                                              <  2:613807/140‑1 (MQ=255)                                      ttCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAg                                                                                         >  2:129784/1‑140 (MQ=255)                                        cGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAgcg                                                                                       <  1:586295/140‑1 (MQ=255)                                        cGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAgcg                                                                                       <  1:361515/140‑1 (MQ=255)                                        cGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAgcg                                                                                       >  2:361515/1‑140 (MQ=255)                                             gccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCcaca                                                                                  >  1:290590/1‑140 (MQ=255)                                                  tGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATAcc                                                                             <  2:8018/140‑1 (MQ=255)                                                     gccgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCgg                                                                          <  1:824684/140‑1 (MQ=255)                                                     gccgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCgg                                                                          <  2:176322/140‑1 (MQ=255)                                                         ccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGcc                                                                      >  1:140616/1‑140 (MQ=255)                                                          cACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCt                                                                     <  2:310795/140‑1 (MQ=255)                                                                      cAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAcg                                                         <  1:82303/140‑1 (MQ=255)                                                                            tttACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTAc                                                   <  1:528875/140‑1 (MQ=255)                                                                             ttACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTAcc                                                  <  2:501704/140‑1 (MQ=255)                                                                             ttACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTAcc                                                  <  1:129784/140‑1 (MQ=255)                                                                              tACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTAc                                                   >  2:83066/1‑138 (MQ=255)                                                                              tACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTAc                                                   <  1:83066/138‑1 (MQ=255)                                                                              tACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCg                                                 <  2:627304/140‑1 (MQ=255)                                                                                 cACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAgg                                              <  2:140616/140‑1 (MQ=255)                                                                                   cGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCt                                            >  2:26649/1‑140 (MQ=255)                                                                                    gAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTa                                           <  1:26649/140‑1 (MQ=255)                                                                                        aGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACa                                       >  2:651445/1‑140 (MQ=255)                                                                                        aGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACa                                       <  2:67960/140‑1 (MQ=255)                                                                                          cATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATa                                     >  2:809946/1‑140 (MQ=255)                                                                                                ggAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTa                                           <  1:582577/128‑1 (MQ=255)                                                                                                ggAAGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTa                                           >  2:582577/1‑128 (MQ=255)                                                                                                  aaGATTTCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGc                             <  1:809946/140‑1 (MQ=255)                                                                                                       ttCCGACAGCAGTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTc                        >  1:433203/1‑140 (MQ=255)                                                                                                                cagTACCCAGCATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTgcgc               >  1:154726/1‑140 (MQ=255)                                                                                                                          cATACCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTg     >  1:620539/1‑140 (MQ=255)                                                                                                                             aCCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAg  <  1:574070/140‑1 (MQ=255)                                                                                                                             aCCGGACTCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAg  >  1:110952/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     TTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAG  >  NC_000913/4240962‑4241225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑