Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,258,984	A→G	T415A (ACG→GCG)	dinF →	DNA damage‑inducible protein F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,258,984	0	A	G	100.0%	58.4 / NA	18	T415A (ACG→GCG)	dinF	DNA damage‑inducible protein F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/18);  total (0/18)

ATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGC  >  NC_000913/4258853‑4259111                                                                                                                                    |                                                                                                                                atcttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCtt                                                                                                                         <  1:829304/140‑1 (MQ=255) atcttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGGGGCTCCtt                                                                                                                         >  1:482542/1‑140 (MQ=255)   cttatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTAc                                                                                                                       <  1:419371/140‑1 (MQ=255)     tatctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGGGGCTCCTTTCGc                                                                                                                     >  1:489460/1‑140 (MQ=255)      atctGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTTCGCTGGGGCCCCTTTCGCt                                                                                                                    >  2:49629/1‑140 (MQ=255)          ggCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGGGGGCCCCCCAGGGTTTTGGCTCGCGCTCCCTTCgccgccc                                                                                                                >  1:662611/1‑139 (MQ=255)            cAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTTCGCTGGGGCTCCTTACGCTGCCggg                                                                                                              >  2:243392/1‑138 (MQ=255)            cAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTTCGCTGGCGCTCCCTACGCTGCCGTg                                                                                                              >  2:672583/1‑140 (MQ=255)            cAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCTGGCGCTCCTTTCGCTGCCGTg                                                                                                              >  2:8352/1‑140 (MQ=255)             aGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTgg                                                                                                             <  2:647622/140‑1 (MQ=255)              ggTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCCGGGTTTTCGCTGGGGCTCCTTACGCTGCCGTggg                                                                                                            >  2:611944/1‑139 (MQ=255)                tGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTg                                                                                                          <  1:116872/140‑1 (MQ=255)                        ccGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCa                                                                                                  <  1:63000/140‑1 (MQ=255)                         cGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTTCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGGAATCAt                                                                                                 >  1:493995/1‑140 (MQ=255)                              gtTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCttt                                                                                            <  1:287538/140‑1 (MQ=255)                                  gCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtgg                                                                                        <  1:293908/140‑1 (MQ=255)                                  gCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtgg                                                                                        <  1:378326/140‑1 (MQ=255)                                     tCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTGCGCTGGGGCCCCTTACGCTGCCGTGGCCGGGGAAACATGCTTTTtgggtg                                                                                     >  2:3936/1‑140 (MQ=255)                                          tgtTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCAtt                                                                                <  1:243392/140‑1 (MQ=255)                                                ctgctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGt                                                                          <  1:8352/140‑1 (MQ=255)                                                     ggACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTc                                                                     <  1:3936/140‑1 (MQ=255)                                                               tttATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTgcg                                                           <  1:611944/140‑1 (MQ=255)                                                                         cAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGGTTTGCGCTGGCGCTCCCTTCGCCGCCGGGGGTGGGGAATCATGCTTTTTGGCTGGGATTTAACGTCTTTTTTGGGGTGGGCGGGGttttt                                                 >  2:67934/1‑138 (MQ=255)                                                                              cgTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTggc                                            <  2:662611/140‑1 (MQ=255)                                                                                                 aCAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCAc                         <  1:67934/140‑1 (MQ=255)                                                                                                      aTGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGgcg                    <  2:399547/140‑1 (MQ=255)                                                                                                      aTGGCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATACTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGgcg                    <  1:855505/140‑1 (MQ=255)                                                                                                        ggCGGTGGCCGCCGCAGGTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGTGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCa                  <  2:734510/140‑1 (MQ=255)                                                                                                             tGGCCGCCGCAGGGTTTTCGCTGGCGCTCCTTACGCTGCCGGGGCTGGGGAATCATGCTTTGTGGGTGGGCTTTAACGTCTTTTTGGCGTTGCGCGGGCTTTTTCTGGCGGCTATCTGGCGGGGGCACTGGGGCCATggg             >  2:19804/1‑139 (MQ=255)                                                                                                                        ggTTTTGCGCTGGCGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGc  <  1:672583/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                ATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGC  >  NC_000913/4258853‑4259111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑