Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,346,487	G→A	A280A (GCC→GCT)	fumB ←	fumarase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,346,487	0	G	A	100.0%	136.6 / NA	39	A280A (GCC→GCT)	fumB	fumarase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/24);  total (15/24)

CTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGA  >  NC_000913/4346351‑4346618                                                                                                                                         |                                                                                                                                    cTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGcc                                                                                                                                  <  2:294070/140‑1 (MQ=255)    tAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCTCCGTGACGTGGCAGACGGATAACGCTAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCctct                                                                                                                               >  1:503217/1‑140 (MQ=255)      aTGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTc                                                                                                                             <  2:446208/140‑1 (MQ=255)          tACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCcagc                                                                                                                         <  2:838130/140‑1 (MQ=255)          tACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCcagc                                                                                                                         <  2:269378/140‑1 (MQ=255)           aCGGTCAGCGGAGCAGGAGACGCCCATGCCGACTGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCcagca                                                                                                                        >  1:627451/1‑140 (MQ=255)              gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGtt                                                                                                                     <  1:717053/140‑1 (MQ=255)              gTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGtt                                                                                                                     <  1:788235/140‑1 (MQ=255)                cAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTcc                                                                                                                   >  1:855635/1‑140 (MQ=255)                   cGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCTCCGTGACGTGGCAGACGGATAACGCTAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGt                                                                                                                <  2:503217/140‑1 (MQ=255)                   cGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGt                                                                                                                <  2:734225/140‑1 (MQ=255)                   cGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGt                                                                                                                >  2:602385/1‑140 (MQ=255)                        cAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAg                                                                                                           >  1:517845/1‑140 (MQ=255)                          ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCt                                                                                                         <  2:698828/140‑1 (MQ=255)                                gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACAt                                                                                                   <  1:534163/140‑1 (MQ=255)                                gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACAt                                                                                                   >  2:635415/1‑140 (MQ=255)                                gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACAt                                                                                                   <  1:217692/140‑1 (MQ=255)                                     tGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAACGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCgg                                                                                              >  2:818264/1‑140 (MQ=255)                                      gCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGa                                                                                             <  2:114529/140‑1 (MQ=255)                                          aCCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGc                                                                                         <  2:521147/140‑1 (MQ=255)                                           ccGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGcc                                                                                        <  2:855635/140‑1 (MQ=255)                                               gCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGAc                                                                                    <  1:290577/140‑1 (MQ=255)                                                cAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAACGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGAcc                                                                                   <  1:818264/140‑1 (MQ=255)                                                        gcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATgttcgt                                                                           >  1:861787/1‑140 (MQ=255)                                                        gcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATgttcgt                                                                           >  2:592551/1‑140 (MQ=255)                                                         ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATgttcgtt                                                                          >  1:406715/1‑140 (MQ=255)                                                           gTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTcc                                                                        <  2:475931/140‑1 (MQ=255)                                                             gACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCt                                                                      >  1:357082/1‑140 (MQ=255)                                                                        cGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAg                                                           <  2:357082/140‑1 (MQ=255)                                                                          gATAACGCGACTGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGtt                                                         <  1:592551/140‑1 (MQ=255)                                                                            tAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCGTGTTCGTTCCCTTCCGTCGGCAGTTCa                                                       <  1:864435/140‑1 (MQ=255)                                                                            tAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCa                                                       <  2:517845/140‑1 (MQ=255)                                                                                     tGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAg                                              <  2:861787/140‑1 (MQ=255)                                                                                               gAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTg                                    <  2:627451/140‑1 (MQ=255)                                                                                                           gCCAAACTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACgg                        >  2:345457/1‑140 (MQ=255)                                                                                                               aaCTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGtttt                    >  1:494336/1‑140 (MQ=255)                                                                                                               aaCTGCGCGCCAAGACCGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGtttt                    >  1:551757/1‑140 (MQ=255)                                                                                                                              ccGAGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGc     <  1:345457/140‑1 (MQ=255)                                                                                                                                 aGTTTCTGAGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGa  >  2:602694/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    CTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGA  >  NC_000913/4346351‑4346618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑