Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,661	A→G	G199G (GGA→GGG)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,661	0	A	G	100.0%	192.3 / NA	55	G199G (GGA→GGG)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

AACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGG  >  NC_000913/4396528‑4396797                                                                                                                                      |                                                                                                                                         aaCCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCAcggc                                                                                                                                    <  2:556439/140‑1 (MQ=255)   ccGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCAcggcgg                                                                                                                                  >  2:679221/1‑140 (MQ=255)         cgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCA‑CAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGAc                                                                                                                           >  1:197621/1‑140 (MQ=255)             aTCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCt                                                                                                                        <  2:648310/140‑1 (MQ=255)               ccGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTgg                                                                                                                      <  2:316124/140‑1 (MQ=255)               ccGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTgg                                                                                                                      <  1:466549/140‑1 (MQ=255)                   ttAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCt                                                                                                                  <  1:286888/140‑1 (MQ=255)                      aaaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATc                                                                                                               <  2:633598/140‑1 (MQ=255)                         cGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGc                                                                                                            <  2:222297/140‑1 (MQ=255)                           gAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGccc                                                                                                          >  1:146102/1‑140 (MQ=255)                           gAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGccc                                                                                                          >  1:675802/1‑140 (MQ=255)                                     gCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcg                                                                                                >  1:204432/1‑140 (MQ=255)                                     gCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcg                                                                                                <  1:7795/140‑1 (MQ=255)                                             ggTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGGCCCGGTGGTACGCGGGAGaa                                                                                        >  1:634143/1‑140 (MQ=255)                                                   aTCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGt                                                                                  >  1:247510/1‑140 (MQ=255)                                                           tAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccaccg                                                                          >  2:463045/1‑140 (MQ=255)                                                             aTACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATg                                                                                   <  2:714095/129‑1 (MQ=255)                                                             aTACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATg                                                                                   >  1:714095/1‑129 (MQ=255)                                                               aCGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgccat                                                                      <  2:247510/140‑1 (MQ=255)                                                                  gTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACcatcgccatcgc                                                                   >  2:512948/1‑140 (MQ=255)                                                                  gTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACcatcgccatcgc                                                                   >  1:174536/1‑140 (MQ=255)                                                                   tAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTTGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATcgcg                                                                  >  2:70136/1‑140 (MQ=255)                                                                     aCGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATcgcgcg                                                                >  2:833341/1‑140 (MQ=255)                                                                     aCGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATcgcgcg                                                                >  1:571590/1‑140 (MQ=255)                                                                     aCGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATcgcgcg                                                                >  1:93145/1‑140 (MQ=255)                                                                      cgcgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGt                                                               <  2:197621/140‑1 (MQ=255)                                                                        cgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgccat                                                                      >  1:799248/1‑131 (MQ=255)                                                                        cgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAccatcgccat                                                                      <  2:799248/131‑1 (MQ=255)                                                                        cgTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTaa                                                             >  1:239625/1‑140 (MQ=255)                                                                                  cgcgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACt                                                   <  1:780513/140‑1 (MQ=255)                                                                                  cgcgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACt                                                   >  1:531596/1‑140 (MQ=255)                                                                                    cgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACttt                                                 >  2:57602/1‑140 (MQ=255)                                                                                    cgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACt                                                   <  1:159046/138‑1 (MQ=255)                                                                                    cgcgcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACt                                                   >  2:159046/1‑138 (MQ=255)                                                                                       gcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCt                                              <  2:93145/140‑1 (MQ=255)                                                                                        cgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTc                                             <  1:453283/140‑1 (MQ=255)                                                                                                    cTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTCTCGGCCCCGGTGGTACGCGGGAGAAAAATGTCCCCATCGTCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCg                                 <  1:635750/140‑1 (MQ=255)                                                                                                    cTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCg                                 <  1:824723/140‑1 (MQ=255)                                                                                                    cTGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCg                                 <  1:270701/140‑1 (MQ=255)                                                                                                     tGGCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGa                                >  2:654939/1‑140 (MQ=255)                                                                                                       gCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGa                                        >  2:104126/1‑130 (MQ=255)                                                                                                       gCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGa                                        <  1:104126/130‑1 (MQ=255)                                                                                                       gCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATg                              >  1:180573/1‑140 (MQ=255)                                                                                                       gCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATg                              >  1:810090/1‑140 (MQ=255)                                                                                                       gCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAG‑AAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGt                             >  2:639245/1‑140 (MQ=255)                                                                                                       gCGATAAAATTATCATCGCTATTGATGCCGGGCACGGCGGTCAAGACCCTGGCGGTATCGGCCCCGGTGGGACGCGGGGGCAAAATGTCACCATCGCCACCGCGCGTAAATTGCCGACTTTGCTCAAAGACGATCCGATg                              >  2:745624/1‑140 (MQ=255)                                                                                                              aaTTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAg                       <  2:204432/140‑1 (MQ=255)                                                                                                               aTTATCATCGCTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAgg                      <  1:463045/140‑1 (MQ=255)                                                                                                                         cTATTGATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAAcc            >  1:227679/1‑140 (MQ=255)                                                                                                                              gATGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGa       <  1:745624/140‑1 (MQ=255)                                                                                                                               aTGCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGAc      <  1:847648/140‑1 (MQ=255)                                                                                                                                tGCCGGGCACCGCGTTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACg     <  2:533197/140‑1 (MQ=255)                                                                                                                                 gCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACgg    >  1:723385/1‑140 (MQ=255)                                                                                                                                 gCCGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATACGATGTTTAAAGGCGTTTTAACCCGTGACgg    >  1:609483/1‑140 (MQ=255)                                                                                                                                  ccGGGCACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAG‑AAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACgggg  <  1:639245/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         AACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGG  >  NC_000913/4396528‑4396797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑