Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,405,247	T→C	V187V (GTT→GTC)	purA →	adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,405,247	0	T	C	100.0%	175.2 / NA	49	V187V (GTT→GTC)	purA	adenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

CACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGG  >  NC_000913/4405114‑4405374                                                                                                                                      |                                                                                                                                cACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGgat                                                                                                                           >  1:369041/1‑140 (MQ=255)  aCGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGgatt                                                                                                                          >  2:90049/1‑139 (MQ=255)   cGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGgatta                                                                                                                         <  1:90049/140‑3 (MQ=255)    gTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGgatgat                                                                                                                        <  1:38383/140‑1 (MQ=255)        cgGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGa                                                                                                                    >  1:292280/1‑140 (MQ=255)           tCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATgg                                                                                                                 <  2:369041/140‑1 (MQ=255)               cGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGt                                                                                                             >  1:237921/1‑140 (MQ=255)                         aCCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATc                                                                                                   <  1:724081/140‑1 (MQ=255)                         aCCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATc                                                                                                   <  1:449633/140‑1 (MQ=255)                         aCCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATc                                                                                                   <  2:338111/140‑1 (MQ=255)                         aCCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATc                                                                                                   >  2:415001/1‑140 (MQ=255)                           cTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCt                                                                                                 <  2:455433/140‑1 (MQ=255)                           cTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCt                                                                                                 <  1:143060/140‑1 (MQ=255)                           cTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCt                                                                                                 <  2:292280/140‑1 (MQ=255)                           cTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAACCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCt                                                                                                 <  2:198289/140‑1 (MQ=255)                              ttCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGAc                                                                                              <  2:75105/140‑1 (MQ=255)                                cGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACtt                                                                                            >  2:673962/1‑140 (MQ=255)                                    aaaGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTAt                                                                                        <  2:2570/140‑1 (MQ=255)                                             ttCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGa                                                                               <  1:209306/140‑1 (MQ=255)                                             ttCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGa                                                                               <  2:237921/140‑1 (MQ=255)                                             ttCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGa                                                                               <  1:746069/140‑1 (MQ=255)                                                       aaCTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGAc                                                                     <  1:115815/140‑1 (MQ=255)                                                       aaCTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGAc                                                                     <  1:599482/140‑1 (MQ=255)                                                         cTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCt                                                                   >  1:448076/1‑140 (MQ=255)                                                            aaaGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCt                                                                >  2:709384/1‑140 (MQ=255)                                                            aaaGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCt                                                                >  2:693575/1‑140 (MQ=255)                                                                    gATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAgg                                                        <  1:121742/140‑1 (MQ=255)                                                                      tGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGgcg                                                      >  1:860480/1‑140 (MQ=255)                                                                                     tCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGAt                                       >  1:239107/1‑140 (MQ=255)                                                                                         gTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCg                                   <  1:693575/140‑1 (MQ=255)                                                                                              ttAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATg                              >  2:808538/1‑140 (MQ=255)                                                                                                  ctactaCAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTg                          >  2:162369/1‑140 (MQ=255)                                                                                                    actacAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGaa                        <  1:709384/140‑1 (MQ=255)                                                                                                    actacAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGaa                        >  2:502481/1‑140 (MQ=255)                                                                                                     ctacAAAGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAg                       <  1:714639/140‑1 (MQ=255)                                                                                                          aaGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgcg                  <  1:808538/140‑1 (MQ=255)                                                                                                          aaGCTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgcg                  <  1:415001/140‑1 (MQ=255)                                                                                                             cTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAg               >  1:221363/1‑140 (MQ=255)                                                                                                             cTGAAGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAg               >  1:697903/1‑140 (MQ=255)                                                                                                                aaGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgc                   <  2:110482/133‑1 (MQ=255)                                                                                                                aaGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTgc                   >  1:110482/1‑133 (MQ=255)                                                                                                                aaGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGt            >  2:335059/1‑140 (MQ=255)                                                                                                                aaGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGt            >  2:497873/1‑140 (MQ=255)                                                                                                                 aGCGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTa           <  2:239107/140‑1 (MQ=255)                                                                                                                   cGGTTGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACg         <  2:448076/140‑1 (MQ=255)                                                                                                                       tGATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctgc     >  2:243668/1‑140 (MQ=255)                                                                                                                        gATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctgct    <  1:673962/140‑1 (MQ=255)                                                                                                                        gATTACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACgctgct    <  2:576483/140‑1 (MQ=255)                                                                                                                          ttACCAGAAAGTCCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTgg  >  1:431753/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                CACGTCGCGGTCTGCGTGTTGGCGACCTTTTCGACAAAGAAACCTTCGCTGAAAAACTGAAAGAAGTGATGGAATATCACAACTTCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGG  >  NC_000913/4405114‑4405374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑