Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	445,979	A→G	S230P (TCG→CCG)	yajR ←	putative major facilitator superfamily transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,979	0	A	G	100.0%	118.4 / NA	35	S230P (TCG→CCG)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

CTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGG  >  NC_000913/445845‑446108                                                                                                                                       |                                                                                                                                  cTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCa                                                                                                                              <  1:747952/140‑1 (MQ=255)   tAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGc                                                                                                                            <  1:3962/140‑1 (MQ=255)    aaCTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGGAGGGCAACAAACGTCGGCATCAGCa                                                                                                                           <  1:825329/140‑1 (MQ=255)     aCTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCaa                                                                                                                          <  2:78533/140‑1 (MQ=255)      cTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCAGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCaaa                                                                                                                         <  2:105904/140‑1 (MQ=255)         cAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAata                                                                                                                      >  2:568705/1‑140 (MQ=255)              tAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAg                                                                                                                 >  1:105940/1‑140 (MQ=255)               aGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGa                                                                                                                <  1:725815/140‑1 (MQ=255)                      gAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATaa                                                                                                         <  2:740102/140‑1 (MQ=255)                          ggCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGcc                                                                                                     >  2:313778/1‑140 (MQ=255)                           gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCa                                                                                                    >  2:59397/1‑140 (MQ=255)                                           ggCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTCGAGTTTcagca                                                                                    >  1:148050/1‑140 (MQ=255)                                                   gCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGt                                                                            >  1:293030/1‑140 (MQ=255)                                                        gTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTCGAGTTTCAGCAGCCGCGGTTCCGc                                                                       <  2:148050/140‑1 (MQ=255)                                                         tCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGcc                                                                      >  1:727641/1‑140 (MQ=255)                                                                   gACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTAc                                                            <  2:727641/140‑1 (MQ=255)                                                                    aCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCa                                                                  <  1:631781/133‑1 (MQ=255)                                                                    aCCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCa                                                                  >  2:631781/1‑133 (MQ=255)                                                                        tCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGaaa                                                       <  1:313778/140‑1 (MQ=255)                                                                         ccAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAAc                                                      >  2:484355/1‑140 (MQ=255)                                                                          cAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGcc                                                                      <  2:275847/123‑1 (MQ=255)                                                                          cAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGcc                                                                      >  1:275847/1‑123 (MQ=255)                                                                             tgtTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGcc                                                  <  1:484355/140‑1 (MQ=255)                                                                                              cccTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACt                                 <  1:650868/140‑1 (MQ=255)                                                                                              cccTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACt                                 <  1:59397/140‑1 (MQ=255)                                                                                              cccTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACt                                 <  2:590655/140‑1 (MQ=255)                                                                                                 tGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACt                                 >  1:566165/1‑137 (MQ=255)                                                                                                 tGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACt                                 <  2:566165/137‑1 (MQ=255)                                                                                                 tGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCAc                              >  2:862258/1‑140 (MQ=255)                                                                                                   cATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGa                            <  2:94507/140‑1 (MQ=255)                                                                                                        gCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGAc                                  <  2:110290/129‑1 (MQ=255)                                                                                                        gCCAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGAc                                  >  1:110290/1‑129 (MQ=255)                                                                                                         ccAGTTGTCCGGGCAGGGCAACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGt                      >  2:257630/1‑140 (MQ=255)                                                                                                                         ggcaACAAACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGt      >  2:722016/1‑140 (MQ=255)                                                                                                                             acaaACGTCGGCATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTggg  <  2:293030/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  CTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGG  >  NC_000913/445845‑446108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑