Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,411,727	A→G	E142E (GAA→GAG)	yjfK →	conserved protein YjfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,411,727	0	A	G	100.0%	232.1 / NA	66	E142E (GAA→GAG)	yjfK	conserved protein YjfK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

AGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAACCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATATTTGCTGCTAA  >  NC_000913/4411591‑4411860                                                                                                                                         |                                                                                                                                      aGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCg                                                                                                                                    >  2:794959/1‑140 (MQ=255)    tctTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGa                                                                                                                                 <  2:486237/140‑1 (MQ=255)        tGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAata                                                                                                                             >  2:73607/1‑140 (MQ=255)        tGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAata                                                                                                                             >  1:208461/1‑140 (MQ=255)        tGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAata                                                                                                                             >  1:726243/1‑140 (MQ=255)           cTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCg                                                                                                                          <  1:384345/140‑1 (MQ=255)             aTGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGaa                                                                                                                        <  2:726243/140‑1 (MQ=255)             aTGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGaa                                                                                                                        <  2:680746/140‑1 (MQ=255)             aTGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGaa                                                                                                                        <  2:208461/140‑1 (MQ=255)              tGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAAc                                                                                                                       >  2:241053/1‑140 (MQ=255)              tGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGAGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAAc                                                                                                                       >  2:196344/1‑140 (MQ=255)                    gagCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGaa                                                                                                                        >  2:840818/1‑133 (MQ=255)                    gagCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGaa                                                                                                                        <  1:840818/133‑1 (MQ=255)                     agCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGa                                                                                                                                 >  2:756384/1‑123 (MQ=255)                     agCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGa                                                                                                                                 <  1:756384/123‑1 (MQ=255)                      gCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTAc                                                                                                               >  1:402022/1‑140 (MQ=255)                       cTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACa                                                                                                              >  2:189026/1‑140 (MQ=255)                        tACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACAt                                                                                                             >  1:126826/1‑140 (MQ=255)                          cGGTATCAGCAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGc                                                                                                           >  1:484738/1‑140 (MQ=255)                            gTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTg                                                                                                         >  2:703145/1‑140 (MQ=255)                              aTCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGa                                                                                                       <  2:357526/140‑1 (MQ=255)                               tCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAAcc                                                                                                                      <  1:39831/124‑1 (MQ=255)                               tCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAAcc                                                                                                                      >  2:39831/1‑124 (MQ=255)                               tCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGaa                                                                                                      >  2:669057/1‑140 (MQ=255)                                  gTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGaaaaa                                                                                                   >  1:400490/1‑140 (MQ=255)                                      agagagTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAg                                                                                               <  2:501993/140‑1 (MQ=255)                                       gagagTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGTTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGa                                                                                              <  1:129031/140‑1 (MQ=255)                                       gagagTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGa                                                                                              <  1:73607/140‑1 (MQ=255)                                       gagagTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGa                                                                                              <  1:546820/140‑1 (MQ=255)                                         gagTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGaaa                                                                                            >  1:512432/1‑140 (MQ=255)                                          agTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTTGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGaaaa                                                                                           >  1:817799/1‑140 (MQ=255)                                          agTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGaaaa                                                                                           >  2:678430/1‑140 (MQ=255)                                           gTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAAt                                                                                          >  2:692926/1‑140 (MQ=255)                                           gTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAAt                                                                                          >  2:746337/1‑140 (MQ=255)                                            tCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATc                                                                                         >  1:617478/1‑140 (MQ=255)                                            tCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATc                                                                                         >  1:545888/1‑140 (MQ=255)                                             cACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCa                                                                                        >  1:648975/1‑140 (MQ=255)                                             cACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCa                                                                                        >  2:488781/1‑140 (MQ=255)                                                   cgcgAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCa                                                                                  <  2:545888/140‑1 (MQ=255)                                                      gAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCaaaa                                                                                     <  2:728233/134‑1 (MQ=255)                                                      gAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCaaaa                                                                                     >  1:728233/1‑134 (MQ=255)                                                            aTCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAACGTAGAAAATCAAAACCATGCCAAATg                                                                         >  2:295436/1‑140 (MQ=255)                                                            aTCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATg                                                                         >  1:417001/1‑140 (MQ=255)                                                            aTCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATg                                                                         >  1:788682/1‑140 (MQ=255)                                                              cAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATggg                                                                       <  1:859880/140‑1 (MQ=255)                                                              cAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATggg                                                                       <  1:794959/140‑1 (MQ=255)                                                                  gCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCa                                                                                  <  1:50981/125‑1 (MQ=255)                                                                  gCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCa                                                                                  >  2:50981/1‑125 (MQ=255)                                                                   ccAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTc                                                                  <  1:692926/140‑1 (MQ=255)                                                                               gAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTAcc                                                      <  2:402022/140‑1 (MQ=255)                                                                                aGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCa                                                     <  2:512432/140‑1 (MQ=255)                                                                                aGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCa                                                     <  2:344631/140‑1 (MQ=255)                                                                                    aTGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATggg                                                 <  1:678430/140‑1 (MQ=255)                                                                                         cTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTAcc                                            <  2:417001/140‑1 (MQ=255)                                                                                         cTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTAcc                                            <  2:10022/140‑1 (MQ=255)                                                                                                tGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCa                                     <  2:788682/140‑1 (MQ=255)                                                                                                  gCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAg                                   >  2:266561/1‑140 (MQ=255)                                                                                                  gCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAg                                   <  2:840420/140‑1 (MQ=255)                                                                                                   cAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGt                                  >  2:797027/1‑140 (MQ=255)                                                                                                    aGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAACGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTa                                 <  1:295436/140‑1 (MQ=255)                                                                                                            cGCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGa                         <  1:403261/140‑1 (MQ=255)                                                                                                             gCTGGCAGCGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGAt                        <  2:400490/140‑1 (MQ=255)                                                                                                                     cGATTTTTTAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGa                <  1:669057/140‑1 (MQ=255)                                                                                                                          ttttAACAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATAtt           <  1:266561/140‑1 (MQ=255)                                                                                                                               aCAGCGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATATTtgctg      <  1:189026/140‑1 (MQ=255)                                                                                                                                   cGAAGAGCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATATTTGCTGCTaa  <  2:817799/140‑1 (MQ=255)                                                                                                                                     aagagCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATAtt           <  1:653856/129‑1 (MQ=255)                                                                                                                                     aagagCCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATAtt           >  2:653856/1‑129 (MQ=255)                                                                                                                                         |                                                                                                                                      AGCTCTTTGTCTATGAAGAGAGCTACGGTATCAGTAAAGAGAGTCACTGGCGCGAGGCGATCAACGCCAAAGCGATGGGAGCAATGACCTTAAACTGGCAGGAAAAACGCTGGCAGCGATTTTTTAACAGCGAAGAACCGGGAAATATCGAACCCGTCTACATGCTGGAAAAAGTAGAAAATCAAAACCATGCCAAATGGGAGGTCCATAATTTTACCATGGGCTACCAACGGCAAGTAACCGAAGATACTTACGAATATTTGCTGCTAA  >  NC_000913/4411591‑4411860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑