Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,415,730	A→G	T486A (ACT→GCT)	aidB →	putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,415,730	0	A	G	98.6%	239.8 / ‑4.3	70	T486A (ACT→GCT)	aidB	putative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (38/31);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01

AGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTACTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATG  >  NC_000913/4415596‑4415862                                                                                                                                       |                                                                                                                                     agcaagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtcat                                                                                                                                 <  1:640203/140‑1 (MQ=255)  gcaagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtcatc                                                                                                                                <  1:135350/140‑1 (MQ=255)  gcaagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtcatc                                                                                                                                <  1:274713/140‑1 (MQ=255)   caagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtcatca                                                                                                                               >  1:170253/1‑140 (MQ=255)   caagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtcatca                                                                                                                               >  1:640951/1‑140 (MQ=255)    aagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtc                                                                                                                                   <  2:294161/135‑1 (MQ=255)    aagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCtc                                                                                                                                   >  1:294161/1‑135 (MQ=255)    aagcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTACTCATCAg                                                                                                                              <  2:282093/140‑1 (MQ=255)     agcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGc                                                                                                                             >  2:726296/1‑140 (MQ=255)     agcGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGc                                                                                                                             >  2:135357/1‑140 (MQ=255)       cGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTa                                                                                                                           >  2:832173/1‑140 (MQ=255)                  gACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTggg                                                                                                                >  2:208638/1‑140 (MQ=255)                    cTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCt                                                                                                              >  2:864682/1‑140 (MQ=255)                    cTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCt                                                                                                              >  1:494060/1‑140 (MQ=255)                    cTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCt                                                                                                              >  1:297789/1‑140 (MQ=255)                       aTTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCg                                                                                                           >  1:284289/1‑140 (MQ=255)                       aTTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCg                                                                                                           <  1:76126/140‑1 (MQ=255)                       aTTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCg                                                                                                           >  1:703893/1‑140 (MQ=255)                       aTTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCg                                                                                                           >  1:660389/1‑140 (MQ=255)                             ggAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTgcgc                                                                                                     <  1:208638/140‑1 (MQ=255)                                aGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCaaa                                                                                                  >  2:390916/1‑140 (MQ=255)                                   aTTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGt                                                                                               <  2:546383/140‑1 (MQ=255)                                     ttGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTg                                                                                             <  1:832173/140‑1 (MQ=255)                                                aaaGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTtc                                                                                  >  1:61609/1‑140 (MQ=255)                                                  aGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTtctc                                                                                >  1:302461/1‑140 (MQ=255)                                                     gCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGc                                                                             >  1:51235/1‑140 (MQ=255)                                                             gCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGgcg                                                                     <  1:858473/140‑1 (MQ=255)                                                              cTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGgcgc                                                                    <  2:297789/140‑1 (MQ=255)                                                              cTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGgcgc                                                                    >  2:574080/1‑140 (MQ=255)                                                              cTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGgcgc                                                                    >  1:11606/1‑140 (MQ=255)                                                                  tttGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGc                                                                <  1:514753/140‑1 (MQ=255)                                                                   ttGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCg                                                               <  1:659458/140‑1 (MQ=255)                                                                   ttGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCg                                                               >  1:744957/1‑140 (MQ=255)                                                                   ttGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCg                                                               <  1:637927/140‑1 (MQ=255)                                                                   ttGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCg                                                               <  2:494060/140‑1 (MQ=255)                                                                     gATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCgtg                                                             <  2:355687/140‑1 (MQ=255)                                                                     gATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCgtg                                                             <  2:703893/140‑1 (MQ=255)                                                                     gATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCgtg                                                             <  2:302461/140‑1 (MQ=255)                                                                      aTCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCgtgg                                                            >  1:185688/1‑140 (MQ=255)                                                                         gcgcgGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAGATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGgtgt                                                         >  2:797324/1‑140 (MQ=255)                                                                         gcgcgGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGgtgt                                                         >  2:712599/1‑140 (MQ=255)                                                                         gcgcgGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGgtgt                                                         >  2:41494/1‑140 (MQ=255)                                                                           gcgGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCa                                                       >  2:272211/1‑140 (MQ=255)                                                                           gcgGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCa                                                       >  2:121516/1‑140 (MQ=255)                                                                            cgGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAg                                                      <  1:584692/140‑1 (MQ=255)                                                                             ggTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAgg                                                     <  1:712599/140‑1 (MQ=255)                                                                             ggTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAgg                                                     <  2:628936/140‑1 (MQ=255)                                                                              gTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGt                                                    <  1:413238/140‑1 (MQ=255)                                                                              gTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGt                                                    <  1:135357/140‑1 (MQ=255)                                                                              gTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGt                                                    <  1:189625/140‑1 (MQ=255)                                                                                             ctgctgcTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATAcgcg                                     >  2:823147/6‑140 (MQ=255)                                                                                                cagcTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCgcgg                                  >  2:753128/1‑140 (MQ=255)                                                                                                cagcTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCgcgg                                  >  1:519788/1‑140 (MQ=255)                                                                                                     gCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCgcggcggcg                             <  1:272211/140‑1 (MQ=255)                                                                                                     gCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCgcggcggcg                             >  1:56017/1‑140 (MQ=255)                                                                                                      cGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGGGGTGCGAAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGt                            <  2:185688/140‑1 (MQ=255)                                                                                                      cGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGt                            <  1:864682/140‑1 (MQ=255)                                                                                                       gTAAGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTa                           >  1:628496/1‑140 (MQ=255)                                                                                                         aaGCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACg                         <  1:673005/140‑1 (MQ=255)                                                                                                           gCCAGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGt                       >  1:491121/1‑140 (MQ=255)                                                                                                              aGCTGAAGAACTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGt                    <  2:170253/140‑1 (MQ=255)                                                                                                                   aagaaCTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCagag               <  1:41494/140‑1 (MQ=255)                                                                                                                   aagaaCTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCagag               <  2:640951/140‑1 (MQ=255)                                                                                                                     gaaCTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTc                   <  1:411619/134‑1 (MQ=255)                                                                                                                     gaaCTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTc                   >  2:411619/1‑134 (MQ=255)                                                                                                                     gaaCTGGGGCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCa             >  2:282770/1‑140 (MQ=255)                                                                                                                           gggCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCa       >  1:60723/1‑140 (MQ=255)                                                                                                                           gggCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCa       >  1:613092/1‑140 (MQ=255)                                                                                                                             gCGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGa     >  2:114701/1‑140 (MQ=255)                                                                                                                              cGAGAGATTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGaa    <  2:628496/140‑1 (MQ=255)                                                                                                                                agagaTTGCTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATg  >  2:103002/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     AGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAACTGGGGCGAGAGATTACTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATG  >  NC_000913/4415596‑4415862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑