Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,191	T→C	Y254C (TAC→TGC)	pepA ←	aminopeptidase A/I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,191	0	T	C	100.0%	182.1 / NA	52	Y254C (TAC→TGC)	pepA	aminopeptidase A/I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/22);  total (30/22)

CATATCGTACTTCATCTCATCCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGTACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTGCTGTAGCTGTCAGC  >  NC_000913/4485070‑4485321                                                                                                                          |                                                                                                                                   caTATCGTACTTCATCTCATCCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCa                                                                                                                  <  1:265210/140‑1 (MQ=255)          cTTCATCTCATCCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGt                                                                                                         <  1:837066/140‑1 (MQ=255)          cTTCATCTCATCCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGt                                                                                                         <  1:410916/140‑1 (MQ=255)            tcatctcatcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGttt                                                                                                       >  1:806711/1‑140 (MQ=255)             catctcatcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGtttt                                                                                                      >  2:280770/1‑140 (MQ=255)              atctcatcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTg                                                                                                     >  2:352382/1‑140 (MQ=255)              atctcatcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTg                                                                                                     <  2:13063/140‑1 (MQ=255)               tctcatcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGc                                                                                                    >  1:165513/1‑140 (MQ=255)                   atcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAAc                                                                                                >  2:238324/1‑140 (MQ=255)                    tcCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAAcc                                                                                               >  1:530782/1‑140 (MQ=255)                         gCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTgac                                                                                          >  2:703396/1‑140 (MQ=255)                             tCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTGCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTgaccgac                                                                                      <  1:441160/140‑1 (MQ=255)                               tGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGaccgaccg                                                                                    <  2:806711/140‑1 (MQ=255)                                      ttGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATa                                                                             >  2:343807/1‑140 (MQ=255)                                        gATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAgg                                                                           >  2:65341/1‑140 (MQ=255)                                        gATCAAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAgg                                                                           >  2:252474/1‑140 (MQ=255)                                         aTCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGa                                                                          >  2:806988/1‑140 (MQ=255)                                           cGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAAt                                                                        <  2:165513/140‑1 (MQ=255)                                            gagaTACCGCTGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATg                                                                       <  1:806988/140‑1 (MQ=255)                                                  ccgccgGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATccc                                                                 <  2:398839/140‑1 (MQ=255)                                                  ccgccgGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATccc                                                                 <  1:660213/140‑1 (MQ=255)                                                   cgccgGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATcccc                                                                >  1:780992/1‑140 (MQ=255)                                                   cgccgGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATcccc                                                                >  1:307385/1‑140 (MQ=255)                                                        gAGTCGAAGGCTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctc                                                           >  1:793888/1‑140 (MQ=255)                                                                  ttAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATctgc                                                 <  1:30420/140‑1 (MQ=255)                                                                       ccTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTc                                            <  2:780992/140‑1 (MQ=255)                                                                               caccaGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATaa                                    >  2:724207/1‑140 (MQ=255)                                                                               caccaGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATaa                                    >  2:474798/1‑140 (MQ=255)                                                                               caccaGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATaa                                    >  2:160322/1‑140 (MQ=255)                                                                                accaGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAAc                                   >  2:372626/1‑140 (MQ=255)                                                                                  caGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAAcgc                                 >  1:763344/1‑140 (MQ=255)                                                                                   aGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAAcgcg                                <  1:830385/140‑1 (MQ=255)                                                                                    gCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCgg                               <  1:65341/140‑1 (MQ=255)                                                                                         aTTGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGtgat                          <  1:343807/140‑1 (MQ=255)                                                                                          ttGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCgg                               <  1:592486/134‑1 (MQ=255)                                                                                          ttGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCgg                               >  2:592486/1‑134 (MQ=255)                                                                                           tGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGtgatga                        <  1:724207/140‑1 (MQ=255)                                                                                           tGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGtgatga                        <  1:474798/140‑1 (MQ=255)                                                                                           tGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGtgatga                        >  1:60142/1‑140 (MQ=255)                                                                                           tGGGCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGtgatga                        <  1:160322/140‑1 (MQ=255)                                                                                            gggCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGAc                       >  2:292750/1‑140 (MQ=255)                                                                                            gggCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGAc                       <  2:793888/140‑1 (MQ=255)                                                                                             ggCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACa                      >  2:433696/1‑140 (MQ=255)                                                                                             ggCGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACa                      >  2:333120/1‑140 (MQ=255)                                                                                               cGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACAtt                    >  1:67342/1‑140 (MQ=255)                                                                                               cGTGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACAtt                    >  1:650517/1‑140 (MQ=255)                                                                                                 tGCATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCt                  <  1:372626/140‑1 (MQ=255)                                                                                                   cATCTTCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTg                >  2:623266/1‑140 (MQ=255)                                                                                                       ttCCGACGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTGCTGt            <  2:67342/140‑1 (MQ=255)                                                                                                            aCGCGTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTGCTGTAGCTg       >  1:835570/1‑140 (MQ=255)                                                                                                               cgTTGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGGGAACCCTGACCGACCGCCGGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTGCTGTAGCTGTca    >  2:772270/1‑140 (MQ=255)                                                                                                                 ttGCCTTTGCACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTGCTGTAGCTGTcagc  >  2:784382/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                   CATATCGTACTTCATCTCATCCATGCCTTCTGAAGGCTTGATCGAGATACCGCCGGAGTCGAAGGTTAAACCTTTACCCACCAGCACGATTGGGCGTGCATCTTCCGACGCGTTGCCTTTGTACTCAATCACCGACATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCTTTCATCTGCTGTTCGCCGATAACGCGGGTGATGACATTCTTGCTGTAGCTGTCAGC  >  NC_000913/4485070‑4485321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑