Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,553,532	C→T	A211V (GCG→GTG)	uxuB →	D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,553,532	0	C	T	100.0%	129.6 / NA	39	A211V (GCG→GTG)	uxuB	D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/17);  total (22/17)

TCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAAC  >  NC_000913/4553397‑4553666                                                                                                                                        |                                                                                                                                       tcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCa                                                                                                                                    >  2:271176/1‑140 (MQ=255) tcgAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCa                                                                                                                                    >  2:240770/1‑140 (MQ=255)      gCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGAt                                                                                                                               <  1:49180/140‑1 (MQ=255)         ttGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTga                                                                                                                            >  2:410974/1‑140 (MQ=255)          tgcgtctgcgtcGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTgaa                                                                                                                           <  1:240770/140‑1 (MQ=255)          tgcgtctgcgtcGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTgaa                                                                                                                           >  2:840107/1‑140 (MQ=255)          tgcgtctgcgtcGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTAC‑GGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTgaag                                                                                                                          >  1:663200/1‑140 (MQ=255)                  cgtcgtGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGt                                                                                                                   >  1:24745/1‑140 (MQ=255)                         aaaaaGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTc                                                                                                            <  1:744046/140‑1 (MQ=255)                           aaaGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTAC‑GGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCg                                                                                                         <  2:663200/140‑1 (MQ=255)                              gggTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTg                                                                                                       <  1:271176/140‑1 (MQ=255)                                gTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCa                                                                                                     <  2:513073/140‑1 (MQ=255)                                  tGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCAcc                                                                                                   >  1:64894/1‑140 (MQ=255)                                                      tCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTcc                                                                               >  1:254756/1‑140 (MQ=255)                                                           cGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCcggcgg                                                                          >  2:557384/1‑140 (MQ=255)                                                                    gcgtgAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAg                                                                 <  1:70191/140‑1 (MQ=255)                                                                     cgtgAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGa                                                                >  2:660700/1‑140 (MQ=255)                                                                     cgtgAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGa                                                                >  1:92313/1‑140 (MQ=255)                                                                                    gtgGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAGCCTTACAGGAAAt                                                 <  1:557384/140‑1 (MQ=255)                                                                                     tgGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAAtt                                                >  1:449894/1‑140 (MQ=255)                                                                                      ggCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTg                                               >  1:335116/1‑140 (MQ=255)                                                                                          aaGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGa                                           >  2:1226/1‑140 (MQ=255)                                                                                          aaGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGa                                           <  2:335116/140‑1 (MQ=255)                                                                                          aaGGTCGCGGTACTGGGGCTGGCCCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTCGACCGCATCGTTCCGGCGGCGACGCGAGAAACCTTACAGGAAATTGCTGa                                           <  2:779864/140‑1 (MQ=255)                                                                                              tCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGa                                           >  1:32550/1‑136 (MQ=255)                                                                                              tCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGa                                           <  2:32550/136‑1 (MQ=255)                                                                                              tCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAg                                       <  2:24745/140‑1 (MQ=255)                                                                                                   gTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTggg                                  <  1:410974/140‑1 (MQ=255)                                                                                                             tggctCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAc                        <  1:660700/140‑1 (MQ=255)                                                                                                              ggctCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAcc                       >  2:404629/1‑140 (MQ=255)                                                                                                              ggctCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAcc                       >  2:450747/1‑140 (MQ=255)                                                                                                              ggctCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAcc                       >  1:803361/1‑140 (MQ=255)                                                                                                              ggctCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAcc                       >  1:775157/1‑140 (MQ=255)                                                                                                                 tCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGt                    <  2:351616/140‑1 (MQ=255)                                                                                                                 tCAGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGt                    <  1:280042/140‑1 (MQ=255)                                                                                                                   aGGCGCGTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCATGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTgc                  >  2:584149/1‑140 (MQ=255)                                                                                                                        cgTGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCAt             >  2:154306/1‑140 (MQ=255)                                                                                                                          tGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAccc                      <  2:76720/129‑1 (MQ=255)                                                                                                                          tGACCCGCAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGAccc                      >  1:76720/1‑129 (MQ=255)                                                                                                                                 cAGCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGa    <  2:254756/140‑1 (MQ=255)                                                                                                                                   gCTGGTGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGGGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGGAc  <  2:402748/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAAC  >  NC_000913/4553397‑4553666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑