Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,636,147 A→G V47V (GTA→GTG)  creB → DNA‑binding transcriptional regulator CreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,1470AG100.0% 140.3 / NA 41V47V (GTA→GTG) creBDNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/18);  total (23/18)

CGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCG  >  NC_000913/4636013‑4636275
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cGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGa                                                                                                                             >  1:127892/1‑140 (MQ=255)
                        gATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCt                                                                                                     >  2:733035/1‑140 (MQ=255)
                             gCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCgg                                                                                                >  1:554149/1‑140 (MQ=255)
                                   gATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTa                                                                                          >  1:697187/1‑140 (MQ=255)
                                      aGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCg                                                                                       >  2:75143/1‑140 (MQ=255)
                                      aGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCg                                                                                       >  1:848922/1‑140 (MQ=255)
                                        ccGACACGCTGGTATACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGc                                                                                     >  1:711983/1‑140 (MQ=255)
                                           acacGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGTCGGATATTAGCGGCttt                                                                                  >  1:62476/1‑140 (MQ=255)
                                             acGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCt                                                                                    >  1:210321/1‑136 (MQ=255)
                                             acGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCt                                                                                    <  2:210321/136‑1 (MQ=255)
                                             acGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGa                                                                                >  1:620878/1‑140 (MQ=255)
                                             acGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGa                                                                                >  2:516827/1‑140 (MQ=255)
                                              cGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGaa                                                                               <  1:118830/140‑1 (MQ=255)
                                               gCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAAt                                                                              <  2:697187/140‑1 (MQ=255)
                                                 tGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATtg                                                                            >  2:624548/1‑140 (MQ=255)
                                                   gTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGTCGGATATTAGCGGCTTTGAATtgtg                                                                          <  2:62476/140‑1 (MQ=255)
                                                   gTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATtgtg                                                                          <  2:539284/140‑1 (MQ=255)
                                                        cATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTgccgcc                                                                     <  2:481488/140‑1 (MQ=255)
                                                             tGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTa                                                                >  2:28500/1‑140 (MQ=255)
                                                             tGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTa                                                                >  2:307943/1‑140 (MQ=255)
                                                             tGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTa                                                                >  1:811295/1‑140 (MQ=255)
                                                                agcagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATtgtg                                                                          >  2:95542/1‑127 (MQ=255)
                                                                agcagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATtgtg                                                                          <  1:95542/127‑1 (MQ=255)
                                                                  cagGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGgc                                                           >  1:39634/1‑140 (MQ=255)
                                                                     gAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCt                                                        <  1:134847/140‑1 (MQ=255)
                                                                     gAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCt                                                        <  1:28500/140‑1 (MQ=255)
                                                                       aGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTcc                                                      >  2:546918/1‑140 (MQ=255)
                                                                         gTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCAt                                                    <  2:554149/140‑1 (MQ=255)
                                                                          ttttGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGt                                                                  >  1:848601/1‑125 (MQ=255)
                                                                          ttttGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGt                                                                  <  2:848601/125‑1 (MQ=255)
                                                                              gCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGc                                               <  2:139124/140‑1 (MQ=255)
                                                                              gCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGc                                               <  2:711983/140‑1 (MQ=255)
                                                                                        tCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTa                                     <  2:811295/140‑1 (MQ=255)
                                                                                                      ttGCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGc                       <  2:620878/140‑1 (MQ=255)
                                                                                                        gCCGGTGCTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGccc                     >  2:693468/1‑140 (MQ=255)
                                                                                                               cTGGATAAAGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGa              >  2:81126/1‑140 (MQ=255)
                                                                                                                       aGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCg      <  1:830003/140‑1 (MQ=255)
                                                                                                                       aGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCg      >  2:830003/1‑140 (MQ=255)
                                                                                                                       aGCTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCg      >  2:237471/1‑140 (MQ=255)
                                                                                                                         cTCGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGAt    <  1:237471/140‑1 (MQ=255)
                                                                                                                           cGCAAGCAGGTGCCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCg  <  1:624548/140‑1 (MQ=255)
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CGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCG  >  NC_000913/4636013‑4636275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑